Experiment: MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4700 and PP_4701 are separated by 206 nucleotides PP_4701 and PP_4702 are separated by 177 nucleotides
PP_4700: PP_4700 - pantothenate synthetase, at 5,341,691 to 5,342,554
_4700
PP_4701: PP_4701 - glucose-6-phosphate isomerase, at 5,342,761 to 5,344,425
_4701
PP_4702: PP_4702 - acetyl-CoA synthetase, at 5,344,603 to 5,346,537
_4702
Position (kb)
5342
5343
5344
5345 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5341.852 kb on + strand, within PP_4700 at 5341.901 kb on + strand, within PP_4700 at 5341.902 kb on - strand, within PP_4700 at 5342.148 kb on - strand at 5342.148 kb on - strand at 5342.148 kb on - strand, within PP_4700 at 5342.148 kb on - strand at 5342.192 kb on - strand, within PP_4700 at 5342.192 kb on - strand, within PP_4700 at 5342.265 kb on - strand, within PP_4700 at 5342.284 kb on + strand, within PP_4700 at 5342.285 kb on - strand at 5342.285 kb on - strand, within PP_4700 at 5342.303 kb on + strand, within PP_4700 at 5342.304 kb on - strand, within PP_4700 at 5342.414 kb on + strand, within PP_4700 at 5342.424 kb on + strand, within PP_4700 at 5342.424 kb on + strand, within PP_4700 at 5342.425 kb on - strand at 5342.425 kb on - strand at 5342.425 kb on - strand at 5342.493 kb on - strand at 5342.526 kb on - strand at 5342.526 kb on - strand at 5342.608 kb on + strand at 5342.609 kb on - strand at 5342.609 kb on - strand at 5342.658 kb on - strand at 5342.685 kb on + strand at 5342.685 kb on + strand at 5342.685 kb on + strand at 5342.685 kb on + strand at 5342.685 kb on + strand at 5342.685 kb on + strand at 5342.686 kb on - strand at 5342.687 kb on + strand at 5342.688 kb on - strand at 5342.688 kb on - strand at 5342.688 kb on - strand at 5342.767 kb on + strand at 5342.767 kb on + strand at 5342.768 kb on - strand at 5342.768 kb on - strand at 5342.770 kb on + strand at 5342.771 kb on - strand at 5342.771 kb on - strand at 5342.775 kb on + strand at 5342.776 kb on - strand at 5342.776 kb on - strand at 5342.776 kb on - strand at 5343.442 kb on - strand, within PP_4701 at 5344.399 kb on + strand at 5344.480 kb on - strand at 5344.480 kb on - strand at 5344.487 kb on - strand at 5344.540 kb on + strand at 5344.540 kb on + strand at 5344.540 kb on + strand at 5344.540 kb on + strand at 5344.541 kb on - strand at 5344.541 kb on - strand at 5344.541 kb on - strand at 5344.551 kb on - strand at 5344.551 kb on - strand at 5344.581 kb on + strand at 5344.581 kb on + strand at 5344.582 kb on - strand at 5344.582 kb on - strand at 5344.582 kb on - strand at 5344.582 kb on - strand at 5344.622 kb on - strand at 5344.673 kb on - strand at 5344.831 kb on + strand, within PP_4702 at 5344.831 kb on + strand, within PP_4702 at 5344.831 kb on + strand, within PP_4702 at 5344.925 kb on - strand, within PP_4702 at 5345.000 kb on + strand, within PP_4702 at 5345.008 kb on + strand, within PP_4702 at 5345.008 kb on + strand, within PP_4702 at 5345.008 kb on + strand, within PP_4702 at 5345.008 kb on + strand, within PP_4702 at 5345.008 kb on + strand, within PP_4702 at 5345.009 kb on - strand, within PP_4702 at 5345.235 kb on + strand, within PP_4702 at 5345.377 kb on + strand, within PP_4702 at 5345.377 kb on + strand, within PP_4702 at 5345.377 kb on + strand, within PP_4702 at 5345.378 kb on - strand, within PP_4702 at 5345.378 kb on - strand, within PP_4702 at 5345.378 kb on - strand, within PP_4702 at 5345.418 kb on + strand, within PP_4702 at 5345.419 kb on - strand, within PP_4702 at 5345.419 kb on - strand, within PP_4702 at 5345.419 kb on - strand, within PP_4702
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL remove 5,341,852 + PP_4700 0.19 -1.8 5,341,901 + PP_4700 0.24 -3.0 5,341,902 - PP_4700 0.24 -2.8 5,342,148 - -1.0 5,342,148 - +0.0 5,342,148 - PP_4700 0.53 -1.9 5,342,148 - -1.5 5,342,192 - PP_4700 0.58 -3.6 5,342,192 - PP_4700 0.58 -2.8 5,342,265 - PP_4700 0.66 -2.3 5,342,284 + PP_4700 0.69 -1.6 5,342,285 - -1.8 5,342,285 - PP_4700 0.69 -2.9 5,342,303 + PP_4700 0.71 -1.3 5,342,304 - PP_4700 0.71 -2.3 5,342,414 + PP_4700 0.84 -2.0 5,342,424 + PP_4700 0.85 -2.1 5,342,424 + PP_4700 0.85 -1.0 5,342,425 - -1.9 5,342,425 - -2.3 5,342,425 - -1.8 5,342,493 - -2.1 5,342,526 - +0.4 5,342,526 - +0.7 5,342,608 + -0.3 5,342,609 - +1.4 5,342,609 - +0.5 5,342,658 - +0.3 5,342,685 + +0.3 5,342,685 + +0.1 5,342,685 + +3.3 5,342,685 + -1.4 5,342,685 + -1.1 5,342,685 + -1.2 5,342,686 - -1.2 5,342,687 + +0.7 5,342,688 - +0.2 5,342,688 - -0.8 5,342,688 - -0.3 5,342,767 + -0.3 5,342,767 + +0.9 5,342,768 - -1.0 5,342,768 - -0.3 5,342,770 + -0.5 5,342,771 - -0.2 5,342,771 - -1.0 5,342,775 + -0.4 5,342,776 - -0.9 5,342,776 - +0.6 5,342,776 - -0.5 5,343,442 - PP_4701 0.41 +0.2 5,344,399 + +0.0 5,344,480 - -0.8 5,344,480 - -2.4 5,344,487 - +0.2 5,344,540 + -0.8 5,344,540 + +0.3 5,344,540 + +0.2 5,344,540 + +1.3 5,344,541 - -0.0 5,344,541 - +0.2 5,344,541 - +0.0 5,344,551 - +0.1 5,344,551 - -0.7 5,344,581 + -2.2 5,344,581 + +0.4 5,344,582 - +1.3 5,344,582 - -1.9 5,344,582 - +0.0 5,344,582 - +0.3 5,344,622 - +0.0 5,344,673 - -1.1 5,344,831 + PP_4702 0.12 -2.2 5,344,831 + PP_4702 0.12 -1.4 5,344,831 + PP_4702 0.12 -0.5 5,344,925 - PP_4702 0.17 -0.8 5,345,000 + PP_4702 0.21 -0.8 5,345,008 + PP_4702 0.21 -0.5 5,345,008 + PP_4702 0.21 -0.7 5,345,008 + PP_4702 0.21 +0.8 5,345,008 + PP_4702 0.21 -0.3 5,345,008 + PP_4702 0.21 +0.3 5,345,009 - PP_4702 0.21 -1.4 5,345,235 + PP_4702 0.33 -0.4 5,345,377 + PP_4702 0.40 -0.3 5,345,377 + PP_4702 0.40 -1.3 5,345,377 + PP_4702 0.40 +0.1 5,345,378 - PP_4702 0.40 +0.3 5,345,378 - PP_4702 0.40 +2.0 5,345,378 - PP_4702 0.40 +0.5 5,345,418 + PP_4702 0.42 -0.8 5,345,419 - PP_4702 0.42 +0.3 5,345,419 - PP_4702 0.42 +1.5 5,345,419 - PP_4702 0.42 -0.1
Or see this region's nucleotide sequence