Experiment: MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3964 and PP_3965 are separated by 63 nucleotides PP_3965 and PP_3966 overlap by 4 nucleotides PP_3966 and PP_3967 are separated by 175 nucleotides PP_3967 and PP_3968 are separated by 5 nucleotides
PP_3964: PP_3964 - transposase, at 4,474,000 to 4,475,535
_3964
PP_3965: PP_3965 - transposase, at 4,475,599 to 4,475,934
_3965
PP_3966: PP_3966 - transposase, at 4,475,931 to 4,476,251
_3966
PP_3967: PP_3967 - MutT/nudix family protein, at 4,476,427 to 4,476,867
_3967
PP_3968: PP_3968 - Sensor histidine kinase, at 4,476,873 to 4,477,991
_3968
Position (kb) 4476
4477 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4475.600 kb on + strand at 4475.611 kb on - strand at 4475.611 kb on - strand at 4475.816 kb on + strand, within PP_3965 at 4476.434 kb on + strand at 4476.434 kb on + strand at 4476.434 kb on + strand at 4476.435 kb on - strand at 4476.695 kb on - strand, within PP_3967 at 4476.720 kb on - strand, within PP_3967 at 4476.720 kb on - strand, within PP_3967 at 4476.798 kb on + strand, within PP_3967 at 4476.799 kb on - strand, within PP_3967 at 4476.833 kb on + strand at 4476.834 kb on - strand at 4476.834 kb on - strand at 4476.834 kb on - strand at 4476.846 kb on + strand at 4476.927 kb on + strand at 4476.928 kb on - strand at 4476.928 kb on - strand at 4476.928 kb on - strand at 4476.969 kb on + strand at 4476.970 kb on - strand at 4476.982 kb on - strand at 4477.016 kb on - strand, within PP_3968 at 4477.016 kb on - strand, within PP_3968 at 4477.025 kb on - strand, within PP_3968 at 4477.230 kb on + strand, within PP_3968 at 4477.248 kb on - strand, within PP_3968 at 4477.338 kb on - strand, within PP_3968 at 4477.385 kb on + strand, within PP_3968 at 4477.385 kb on + strand, within PP_3968 at 4477.386 kb on - strand, within PP_3968 at 4477.386 kb on - strand, within PP_3968 at 4477.409 kb on + strand, within PP_3968 at 4477.468 kb on - strand, within PP_3968 at 4477.485 kb on + strand, within PP_3968 at 4477.485 kb on + strand, within PP_3968 at 4477.486 kb on - strand, within PP_3968 at 4477.530 kb on + strand, within PP_3968 at 4477.564 kb on + strand, within PP_3968 at 4477.565 kb on - strand, within PP_3968 at 4477.671 kb on + strand, within PP_3968 at 4477.671 kb on + strand, within PP_3968 at 4477.672 kb on - strand, within PP_3968 at 4477.731 kb on + strand, within PP_3968 at 4477.742 kb on + strand, within PP_3968 at 4477.780 kb on - strand, within PP_3968 at 4477.780 kb on - strand, within PP_3968 at 4477.832 kb on + strand, within PP_3968 at 4477.833 kb on - strand, within PP_3968 at 4477.833 kb on - strand, within PP_3968 at 4477.839 kb on - strand, within PP_3968 at 4477.839 kb on - strand, within PP_3968
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL remove 4,475,600 + -0.3 4,475,611 - +1.0 4,475,611 - -1.2 4,475,816 + PP_3965 0.65 +0.5 4,476,434 + -1.8 4,476,434 + +0.8 4,476,434 + +0.4 4,476,435 - +0.5 4,476,695 - PP_3967 0.61 -1.5 4,476,720 - PP_3967 0.66 -2.5 4,476,720 - PP_3967 0.66 -0.3 4,476,798 + PP_3967 0.84 -1.2 4,476,799 - PP_3967 0.84 +1.8 4,476,833 + -0.2 4,476,834 - +0.8 4,476,834 - -1.0 4,476,834 - -1.6 4,476,846 + +0.2 4,476,927 + +1.3 4,476,928 - -2.7 4,476,928 - +1.8 4,476,928 - +0.1 4,476,969 + +1.4 4,476,970 - +0.1 4,476,982 - +0.4 4,477,016 - PP_3968 0.13 +1.0 4,477,016 - PP_3968 0.13 +0.3 4,477,025 - PP_3968 0.14 +1.0 4,477,230 + PP_3968 0.32 -0.7 4,477,248 - PP_3968 0.34 -0.0 4,477,338 - PP_3968 0.42 -1.2 4,477,385 + PP_3968 0.46 -1.6 4,477,385 + PP_3968 0.46 +0.5 4,477,386 - PP_3968 0.46 +0.8 4,477,386 - PP_3968 0.46 -0.7 4,477,409 + PP_3968 0.48 -0.5 4,477,468 - PP_3968 0.53 -0.6 4,477,485 + PP_3968 0.55 -2.8 4,477,485 + PP_3968 0.55 -0.7 4,477,486 - PP_3968 0.55 -0.1 4,477,530 + PP_3968 0.59 -0.1 4,477,564 + PP_3968 0.62 -0.6 4,477,565 - PP_3968 0.62 +0.8 4,477,671 + PP_3968 0.71 +0.2 4,477,671 + PP_3968 0.71 +0.7 4,477,672 - PP_3968 0.71 +1.3 4,477,731 + PP_3968 0.77 -1.2 4,477,742 + PP_3968 0.78 -0.6 4,477,780 - PP_3968 0.81 -0.3 4,477,780 - PP_3968 0.81 +0.1 4,477,832 + PP_3968 0.86 -1.0 4,477,833 - PP_3968 0.86 +0.8 4,477,833 - PP_3968 0.86 -0.3 4,477,839 - PP_3968 0.86 +0.9 4,477,839 - PP_3968 0.86 -1.7
Or see this region's nucleotide sequence