Strain Fitness in Pseudomonas putida KT2440 around PP_1990

Experiment: MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1989 and PP_1990 are separated by 703 nucleotidesPP_1990 and PP_1992 are separated by 391 nucleotides PP_1989: PP_1989 - Aspartate-semialdehyde dehydrogenase, at 2,254,828 to 2,255,940 _1989 PP_1990: PP_1990 - transposase, at 2,256,644 to 2,257,945 _1990 PP_1992: PP_1992 - aspartate-semialdehyde dehydrogenase, at 2,258,337 to 2,259,341 _1992 Position (kb) 2256 2257 2258Strain fitness (log2 ratio) -1 0 1 2at 2255.986 kb on + strandat 2255.987 kb on - strandat 2255.995 kb on - strandat 2256.006 kb on + strandat 2256.007 kb on - strandat 2256.007 kb on - strandat 2256.055 kb on + strandat 2256.118 kb on - strandat 2256.376 kb on - strandat 2256.376 kb on - strandat 2256.376 kb on - strandat 2256.399 kb on - strandat 2256.512 kb on - strandat 2256.530 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL
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2,255,986 + -1.0
2,255,987 - +0.2
2,255,995 - +2.7
2,256,006 + +0.1
2,256,007 - +0.4
2,256,007 - -0.3
2,256,055 + +0.6
2,256,118 - +0.4
2,256,376 - +0.1
2,256,376 - -1.0
2,256,376 - +1.1
2,256,399 - +0.8
2,256,512 - +0.3
2,256,530 - +0.7

Or see this region's nucleotide sequence