Experiment: MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1827 and PP_1828 are separated by 49 nucleotides PP_1828 and PP_1829 are separated by 65 nucleotides
PP_1827: PP_1827 - N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase, at 2,051,860 to 2,052,768
_1827
PP_1828: PP_1828 - conserved protein of unknown function, at 2,052,818 to 2,053,600
_1828
PP_1829: PP_1829 - Hydrolase, alpha/beta fold family, at 2,053,666 to 2,054,625
_1829
Position (kb)
2052
2053
2054 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2051.825 kb on + strand at 2051.855 kb on - strand at 2051.855 kb on - strand at 2051.855 kb on - strand at 2051.893 kb on + strand at 2051.894 kb on - strand at 2052.025 kb on + strand, within PP_1827 at 2052.025 kb on + strand, within PP_1827 at 2052.026 kb on - strand, within PP_1827 at 2052.059 kb on + strand, within PP_1827 at 2052.102 kb on - strand, within PP_1827 at 2052.394 kb on + strand, within PP_1827 at 2052.394 kb on + strand, within PP_1827 at 2052.394 kb on + strand, within PP_1827 at 2052.395 kb on - strand, within PP_1827 at 2052.395 kb on - strand, within PP_1827 at 2052.466 kb on + strand, within PP_1827 at 2052.467 kb on - strand, within PP_1827 at 2052.560 kb on + strand, within PP_1827 at 2052.655 kb on + strand, within PP_1827 at 2052.772 kb on - strand at 2052.881 kb on - strand at 2052.915 kb on + strand, within PP_1828 at 2052.915 kb on + strand, within PP_1828 at 2053.075 kb on + strand, within PP_1828 at 2053.434 kb on + strand, within PP_1828 at 2053.435 kb on - strand, within PP_1828 at 2053.502 kb on + strand, within PP_1828 at 2053.618 kb on + strand at 2053.618 kb on + strand at 2053.619 kb on - strand at 2053.619 kb on - strand at 2053.619 kb on - strand at 2053.619 kb on - strand at 2053.619 kb on - strand at 2053.745 kb on + strand at 2053.746 kb on - strand at 2053.746 kb on - strand at 2053.746 kb on - strand at 2053.746 kb on - strand at 2054.276 kb on + strand, within PP_1829 at 2054.276 kb on + strand, within PP_1829 at 2054.277 kb on - strand, within PP_1829 at 2054.557 kb on + strand at 2054.558 kb on - strand at 2054.558 kb on - strand at 2054.558 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL remove 2,051,825 + -1.0 2,051,855 - +0.2 2,051,855 - -1.3 2,051,855 - -4.1 2,051,893 + +0.8 2,051,894 - -2.5 2,052,025 + PP_1827 0.18 -2.6 2,052,025 + PP_1827 0.18 -1.2 2,052,026 - PP_1827 0.18 -1.9 2,052,059 + PP_1827 0.22 -0.9 2,052,102 - PP_1827 0.27 -1.5 2,052,394 + PP_1827 0.59 -1.8 2,052,394 + PP_1827 0.59 -3.4 2,052,394 + PP_1827 0.59 -0.7 2,052,395 - PP_1827 0.59 -1.7 2,052,395 - PP_1827 0.59 -2.6 2,052,466 + PP_1827 0.67 +0.3 2,052,467 - PP_1827 0.67 -1.4 2,052,560 + PP_1827 0.77 +1.8 2,052,655 + PP_1827 0.87 +0.8 2,052,772 - -0.2 2,052,881 - +0.6 2,052,915 + PP_1828 0.12 -0.2 2,052,915 + PP_1828 0.12 -2.0 2,053,075 + PP_1828 0.33 -0.9 2,053,434 + PP_1828 0.79 -2.5 2,053,435 - PP_1828 0.79 +0.4 2,053,502 + PP_1828 0.87 -1.1 2,053,618 + -0.3 2,053,618 + +0.6 2,053,619 - -0.2 2,053,619 - -1.7 2,053,619 - +0.4 2,053,619 - +2.8 2,053,619 - -2.2 2,053,745 + +1.4 2,053,746 - +1.2 2,053,746 - -1.3 2,053,746 - -0.4 2,053,746 - +0.7 2,054,276 + PP_1829 0.64 -0.3 2,054,276 + PP_1829 0.64 +0.4 2,054,277 - PP_1829 0.64 +1.4 2,054,557 + +0.2 2,054,558 - -0.2 2,054,558 - -0.5 2,054,558 - -3.1
Or see this region's nucleotide sequence