Experiment: MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5467 and PP_1248 are separated by 100 nucleotides PP_1248 and PP_1249 are separated by 40 nucleotides PP_1249 and PP_1250 are separated by 504 nucleotides
PP_5467: PP_5467 - conserved exported protein of unknown function, at 1,423,290 to 1,424,498
_5467
PP_1248: PP_1248 - Transporter, LysE family, at 1,424,599 to 1,425,366
_1248
PP_1249: PP_1249 - conserved protein of unknown function, DUF4223 family, at 1,425,407 to 1,425,598
_1249
PP_1250: PP_1250 - Group II intron-encoding maturase, at 1,426,103 to 1,427,524
_1250
Position (kb)
1425
1426 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1424.424 kb on + strand at 1424.424 kb on + strand at 1424.424 kb on + strand at 1424.425 kb on - strand at 1424.425 kb on - strand at 1424.425 kb on - strand at 1424.425 kb on - strand at 1424.425 kb on - strand at 1424.478 kb on + strand at 1424.479 kb on - strand at 1424.479 kb on - strand at 1424.556 kb on + strand at 1424.664 kb on + strand at 1424.664 kb on + strand at 1424.665 kb on - strand at 1424.665 kb on - strand at 1424.665 kb on - strand at 1424.735 kb on + strand, within PP_1248 at 1424.736 kb on - strand, within PP_1248 at 1424.736 kb on - strand, within PP_1248 at 1424.834 kb on + strand, within PP_1248 at 1424.834 kb on + strand, within PP_1248 at 1424.834 kb on + strand, within PP_1248 at 1424.835 kb on - strand, within PP_1248 at 1424.961 kb on - strand, within PP_1248 at 1424.961 kb on - strand, within PP_1248 at 1424.970 kb on - strand, within PP_1248 at 1424.970 kb on - strand, within PP_1248 at 1424.994 kb on + strand, within PP_1248 at 1424.995 kb on - strand, within PP_1248 at 1424.995 kb on - strand, within PP_1248 at 1425.011 kb on + strand, within PP_1248 at 1425.011 kb on + strand, within PP_1248 at 1425.011 kb on + strand, within PP_1248 at 1425.258 kb on + strand, within PP_1248 at 1425.258 kb on + strand, within PP_1248 at 1425.258 kb on + strand, within PP_1248 at 1425.258 kb on + strand, within PP_1248 at 1425.405 kb on + strand at 1425.488 kb on + strand, within PP_1249 at 1425.489 kb on - strand, within PP_1249 at 1425.489 kb on - strand, within PP_1249 at 1425.512 kb on + strand, within PP_1249 at 1425.512 kb on + strand, within PP_1249 at 1425.512 kb on + strand, within PP_1249 at 1425.512 kb on + strand, within PP_1249 at 1425.512 kb on + strand, within PP_1249 at 1425.512 kb on + strand, within PP_1249 at 1425.513 kb on - strand, within PP_1249 at 1425.513 kb on - strand, within PP_1249 at 1425.513 kb on - strand, within PP_1249 at 1425.513 kb on - strand, within PP_1249 at 1425.518 kb on + strand, within PP_1249 at 1425.577 kb on + strand, within PP_1249 at 1425.596 kb on + strand at 1425.597 kb on - strand at 1425.703 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL remove 1,424,424 + -1.2 1,424,424 + +0.1 1,424,424 + +0.8 1,424,425 - -0.6 1,424,425 - -0.3 1,424,425 - -0.0 1,424,425 - -1.1 1,424,425 - -0.4 1,424,478 + -0.1 1,424,479 - +0.1 1,424,479 - -0.8 1,424,556 + -0.6 1,424,664 + +0.4 1,424,664 + -0.1 1,424,665 - -0.0 1,424,665 - -1.7 1,424,665 - -0.3 1,424,735 + PP_1248 0.18 +3.1 1,424,736 - PP_1248 0.18 +1.7 1,424,736 - PP_1248 0.18 -1.2 1,424,834 + PP_1248 0.31 -0.7 1,424,834 + PP_1248 0.31 +0.1 1,424,834 + PP_1248 0.31 -0.0 1,424,835 - PP_1248 0.31 -1.2 1,424,961 - PP_1248 0.47 +2.1 1,424,961 - PP_1248 0.47 -0.2 1,424,970 - PP_1248 0.48 -1.5 1,424,970 - PP_1248 0.48 -0.7 1,424,994 + PP_1248 0.51 -0.5 1,424,995 - PP_1248 0.52 +0.5 1,424,995 - PP_1248 0.52 -1.6 1,425,011 + PP_1248 0.54 +0.2 1,425,011 + PP_1248 0.54 -1.3 1,425,011 + PP_1248 0.54 +1.7 1,425,258 + PP_1248 0.86 -0.2 1,425,258 + PP_1248 0.86 +1.1 1,425,258 + PP_1248 0.86 -0.9 1,425,258 + PP_1248 0.86 +0.6 1,425,405 + -0.1 1,425,488 + PP_1249 0.42 -0.3 1,425,489 - PP_1249 0.43 -0.5 1,425,489 - PP_1249 0.43 -0.6 1,425,512 + PP_1249 0.55 -0.4 1,425,512 + PP_1249 0.55 -0.0 1,425,512 + PP_1249 0.55 -0.5 1,425,512 + PP_1249 0.55 -1.9 1,425,512 + PP_1249 0.55 +1.1 1,425,512 + PP_1249 0.55 -1.8 1,425,513 - PP_1249 0.55 -1.0 1,425,513 - PP_1249 0.55 -0.6 1,425,513 - PP_1249 0.55 -0.9 1,425,513 - PP_1249 0.55 -0.2 1,425,518 + PP_1249 0.58 -1.0 1,425,577 + PP_1249 0.89 -0.4 1,425,596 + -1.2 1,425,597 - +0.3 1,425,703 - +0.9
Or see this region's nucleotide sequence