Experiment: D-2-Aminobutyric (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0300 and PP_0301 are separated by 99 nucleotides PP_0301 and PP_0302 are separated by 10 nucleotides PP_0302 and PP_0303 are separated by 166 nucleotides
PP_0300: PP_0300 - putative metallopeptidase, at 360,350 to 361,936
_0300
PP_0301: PP_0301 - betainyl-CoA thiolase, at 362,036 to 362,506
_0301
PP_0302: PP_0302 - L-carnitine 3-dehydrogenase (EC 1.1.1.108) <small>(from data)</small>, at 362,517 to 363,482
_0302
PP_0303: PP_0303 - dehydrocarnitine cleavage enzyme, at 363,649 to 364,533
_0303
Position (kb) 362
363
364 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 361.538 kb on - strand, within PP_0300 at 361.628 kb on + strand, within PP_0300 at 361.738 kb on - strand, within PP_0300 at 361.805 kb on - strand at 361.854 kb on + strand at 361.855 kb on - strand at 361.917 kb on - strand at 361.928 kb on + strand at 361.957 kb on + strand at 361.958 kb on - strand at 362.000 kb on + strand at 362.005 kb on - strand at 362.005 kb on - strand at 362.005 kb on - strand at 362.005 kb on - strand at 362.056 kb on + strand at 362.066 kb on - strand at 362.067 kb on + strand at 362.068 kb on - strand at 362.068 kb on - strand at 362.100 kb on - strand, within PP_0301 at 362.174 kb on + strand, within PP_0301 at 362.175 kb on - strand, within PP_0301 at 362.232 kb on + strand, within PP_0301 at 362.233 kb on - strand, within PP_0301 at 362.233 kb on - strand, within PP_0301 at 362.313 kb on + strand, within PP_0301 at 362.314 kb on - strand, within PP_0301 at 362.321 kb on - strand, within PP_0301 at 362.409 kb on + strand, within PP_0301 at 362.409 kb on + strand, within PP_0301 at 362.427 kb on + strand, within PP_0301 at 362.454 kb on + strand, within PP_0301 at 362.519 kb on - strand at 362.519 kb on - strand at 362.695 kb on + strand, within PP_0302 at 362.695 kb on + strand, within PP_0302 at 362.696 kb on - strand, within PP_0302 at 362.696 kb on - strand, within PP_0302 at 362.771 kb on + strand, within PP_0302 at 362.771 kb on + strand, within PP_0302 at 362.773 kb on + strand, within PP_0302 at 362.773 kb on + strand, within PP_0302 at 362.773 kb on + strand, within PP_0302 at 362.774 kb on - strand, within PP_0302 at 362.774 kb on - strand, within PP_0302 at 362.774 kb on - strand, within PP_0302 at 362.774 kb on - strand, within PP_0302 at 362.774 kb on - strand, within PP_0302 at 362.774 kb on - strand, within PP_0302 at 362.777 kb on + strand, within PP_0302 at 362.777 kb on + strand, within PP_0302 at 362.777 kb on + strand, within PP_0302 at 362.778 kb on - strand, within PP_0302 at 362.778 kb on - strand, within PP_0302 at 362.778 kb on - strand, within PP_0302 at 362.778 kb on - strand, within PP_0302 at 362.778 kb on - strand, within PP_0302 at 362.969 kb on - strand, within PP_0302 at 362.969 kb on - strand, within PP_0302 at 362.991 kb on - strand, within PP_0302 at 362.991 kb on - strand, within PP_0302 at 363.044 kb on - strand, within PP_0302 at 363.044 kb on - strand, within PP_0302 at 363.100 kb on + strand, within PP_0302 at 363.100 kb on + strand, within PP_0302 at 363.101 kb on - strand, within PP_0302 at 363.101 kb on - strand, within PP_0302 at 363.482 kb on + strand at 363.482 kb on + strand at 363.482 kb on + strand at 363.483 kb on - strand at 363.483 kb on - strand at 363.483 kb on - strand at 363.499 kb on - strand at 363.508 kb on + strand at 363.508 kb on + strand at 363.508 kb on + strand at 363.508 kb on + strand at 363.508 kb on + strand at 363.509 kb on - strand at 363.509 kb on - strand at 363.509 kb on - strand at 363.780 kb on - strand, within PP_0303 at 363.780 kb on - strand, within PP_0303 at 363.780 kb on - strand, within PP_0303 at 364.109 kb on + strand, within PP_0303 at 364.109 kb on + strand, within PP_0303 at 364.110 kb on - strand, within PP_0303 at 364.110 kb on - strand, within PP_0303 at 364.426 kb on + strand, within PP_0303
Per-strain Table
Position Strand Gene LocusTag Fraction D-2-Aminobutyric (N) remove 361,538 - PP_0300 0.75 -1.1 361,628 + PP_0300 0.81 -2.2 361,738 - PP_0300 0.87 +0.3 361,805 - -0.7 361,854 + -1.8 361,855 - +0.6 361,917 - -2.0 361,928 + -0.0 361,957 + +0.9 361,958 - -2.8 362,000 + -0.1 362,005 - -0.0 362,005 - +0.0 362,005 - +1.6 362,005 - +1.0 362,056 + +1.6 362,066 - -0.8 362,067 + +0.8 362,068 - +1.7 362,068 - -2.2 362,100 - PP_0301 0.14 -0.5 362,174 + PP_0301 0.29 +1.2 362,175 - PP_0301 0.30 +2.0 362,232 + PP_0301 0.42 +2.0 362,233 - PP_0301 0.42 +1.7 362,233 - PP_0301 0.42 +1.1 362,313 + PP_0301 0.59 -1.2 362,314 - PP_0301 0.59 -1.4 362,321 - PP_0301 0.61 -0.5 362,409 + PP_0301 0.79 +1.2 362,409 + PP_0301 0.79 +0.9 362,427 + PP_0301 0.83 +2.7 362,454 + PP_0301 0.89 +1.7 362,519 - -1.5 362,519 - +1.1 362,695 + PP_0302 0.18 -2.1 362,695 + PP_0302 0.18 -2.5 362,696 - PP_0302 0.19 +0.8 362,696 - PP_0302 0.19 -2.3 362,771 + PP_0302 0.26 +1.1 362,771 + PP_0302 0.26 +1.4 362,773 + PP_0302 0.27 +0.6 362,773 + PP_0302 0.27 +0.3 362,773 + PP_0302 0.27 -0.1 362,774 - PP_0302 0.27 +1.2 362,774 - PP_0302 0.27 -0.2 362,774 - PP_0302 0.27 +0.1 362,774 - PP_0302 0.27 +1.7 362,774 - PP_0302 0.27 -0.8 362,774 - PP_0302 0.27 -0.7 362,777 + PP_0302 0.27 +1.2 362,777 + PP_0302 0.27 +0.4 362,777 + PP_0302 0.27 +2.5 362,778 - PP_0302 0.27 +0.0 362,778 - PP_0302 0.27 -0.6 362,778 - PP_0302 0.27 -1.1 362,778 - PP_0302 0.27 -1.2 362,778 - PP_0302 0.27 +1.5 362,969 - PP_0302 0.47 +0.9 362,969 - PP_0302 0.47 +1.7 362,991 - PP_0302 0.49 +0.1 362,991 - PP_0302 0.49 -2.3 363,044 - PP_0302 0.55 -1.2 363,044 - PP_0302 0.55 -1.5 363,100 + PP_0302 0.60 +0.8 363,100 + PP_0302 0.60 -0.9 363,101 - PP_0302 0.60 +1.5 363,101 - PP_0302 0.60 -1.5 363,482 + +0.5 363,482 + +0.3 363,482 + +0.4 363,483 - -0.2 363,483 - -0.6 363,483 - +0.6 363,499 - -0.6 363,508 + -0.1 363,508 + +0.4 363,508 + +1.7 363,508 + -0.7 363,508 + -0.6 363,509 - +0.8 363,509 - -0.3 363,509 - +3.3 363,780 - PP_0303 0.15 +0.0 363,780 - PP_0303 0.15 -1.3 363,780 - PP_0303 0.15 -0.3 364,109 + PP_0303 0.52 -1.6 364,109 + PP_0303 0.52 -1.1 364,110 - PP_0303 0.52 -0.3 364,110 - PP_0303 0.52 -1.6 364,426 + PP_0303 0.88 -0.8
Or see this region's nucleotide sequence