Strain Fitness in Pseudomonas putida KT2440 around PP_4801

Experiment: R2A control with 0.2% methanol

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_4800 and PP_4801 are separated by 2 nucleotidesPP_4801 and PP_4802 overlap by 4 nucleotidesPP_4802 and PP_4803 are separated by 73 nucleotides PP_4800: PP_4800 - Lipoyl synthase, at 5,463,448 to 5,464,464 _4800 PP_4801: PP_4801 - Octanoyltransferase, at 5,464,467 to 5,465,117 _4801 PP_4802: PP_4802 - putative lipoate regulatory protein, at 5,465,114 to 5,465,389 _4802 PP_4803: PP_4803 - D-alanyl-D-alanine carboxypeptidase, at 5,465,463 to 5,466,623 _4803 Position (kb) 5464 5465 5466Strain fitness (log2 ratio) -1 0 1at 5464.505 kb on - strandat 5465.407 kb on - strandat 5465.462 kb on - strandat 5465.462 kb on - strandat 5465.488 kb on - strandat 5465.682 kb on - strand, within PP_4803at 5465.688 kb on - strand, within PP_4803at 5465.688 kb on - strand, within PP_4803at 5465.688 kb on - strand, within PP_4803at 5465.688 kb on - strand, within PP_4803at 5465.780 kb on - strand, within PP_4803at 5466.034 kb on + strandat 5466.034 kb on + strand, within PP_4803at 5466.035 kb on - strand, within PP_4803at 5466.043 kb on + strand, within PP_4803at 5466.043 kb on + strand, within PP_4803at 5466.043 kb on + strand, within PP_4803at 5466.107 kb on - strand, within PP_4803

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A control with 0.2% methanol
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5,464,505 - +0.3
5,465,407 - -0.2
5,465,462 - +0.5
5,465,462 - +0.1
5,465,488 - -0.6
5,465,682 - PP_4803 0.19 +0.6
5,465,688 - PP_4803 0.19 -0.7
5,465,688 - PP_4803 0.19 +0.6
5,465,688 - PP_4803 0.19 -0.4
5,465,688 - PP_4803 0.19 +1.4
5,465,780 - PP_4803 0.27 +0.3
5,466,034 + -0.6
5,466,034 + PP_4803 0.49 +0.3
5,466,035 - PP_4803 0.49 +1.1
5,466,043 + PP_4803 0.50 -0.6
5,466,043 + PP_4803 0.50 -1.3
5,466,043 + PP_4803 0.50 -0.3
5,466,107 - PP_4803 0.55 +0.3

Or see this region's nucleotide sequence