Strain Fitness in Pseudomonas putida KT2440 around PP_5666

Experiment: R2A control with 0.2% methanol

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_4238 and PP_5665 are separated by 392 nucleotidesPP_5665 and PP_5666 are separated by 58 nucleotidesPP_5666 and PP_5667 overlap by 1 nucleotidesPP_5667 and PP_4240 are separated by 4 nucleotides PP_4238: PP_4238 - conserved protein of unknown function, at 4,811,757 to 4,812,080 _4238 PP_5665: PP_5665 - putative microcin B17-like protein, McbA family, at 4,812,473 to 4,812,715 _5665 PP_5666: PP_5666 - putative microcin B17-like processing protein, McbB family, at 4,812,774 to 4,813,637 _5666 PP_5667: PP_5667 - putative microcin B17-like-processing protein, McbC family, at 4,813,637 to 4,814,428 _5667 PP_4240: PP_4240 - putative Microcin b17 processing protein mcbd, at 4,814,433 to 4,815,635 _4240 Position (kb) 4812 4813 4814Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 4811.995 kb on + strand, within PP_4238at 4812.017 kb on + strand, within PP_4238at 4812.017 kb on + strand, within PP_4238at 4812.078 kb on + strandat 4812.131 kb on + strandat 4812.150 kb on - strandat 4812.230 kb on + strandat 4812.452 kb on + strandat 4812.453 kb on - strandat 4812.523 kb on + strand, within PP_5665at 4812.692 kb on - strandat 4813.050 kb on + strand, within PP_5666at 4813.149 kb on - strand, within PP_5666at 4813.168 kb on + strand, within PP_5666at 4813.229 kb on - strand, within PP_5666at 4813.353 kb on - strand, within PP_5666at 4813.475 kb on - strand, within PP_5666at 4813.632 kb on - strandat 4813.721 kb on + strand, within PP_5667at 4813.721 kb on + strand, within PP_5667at 4813.962 kb on + strand, within PP_5667at 4814.203 kb on + strand, within PP_5667at 4814.207 kb on + strand, within PP_5667at 4814.241 kb on - strandat 4814.288 kb on + strand, within PP_5667at 4814.349 kb on - strandat 4814.350 kb on + strandat 4814.486 kb on + strandat 4814.547 kb on + strandat 4814.547 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A control with 0.2% methanol
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4,811,995 + PP_4238 0.73 +1.1
4,812,017 + PP_4238 0.80 -3.9
4,812,017 + PP_4238 0.80 -0.2
4,812,078 + +0.9
4,812,131 + -1.2
4,812,150 - +0.3
4,812,230 + -2.4
4,812,452 + -3.0
4,812,453 - +0.8
4,812,523 + PP_5665 0.21 -0.3
4,812,692 - +0.0
4,813,050 + PP_5666 0.32 -2.3
4,813,149 - PP_5666 0.43 -0.1
4,813,168 + PP_5666 0.46 -0.4
4,813,229 - PP_5666 0.53 -1.4
4,813,353 - PP_5666 0.67 -1.0
4,813,475 - PP_5666 0.81 -0.4
4,813,632 - -2.6
4,813,721 + PP_5667 0.11 -1.4
4,813,721 + PP_5667 0.11 +0.8
4,813,962 + PP_5667 0.41 -1.9
4,814,203 + PP_5667 0.71 -1.1
4,814,207 + PP_5667 0.72 -1.2
4,814,241 - +0.1
4,814,288 + PP_5667 0.82 +0.1
4,814,349 - -1.7
4,814,350 + -0.7
4,814,486 + -1.5
4,814,547 + -0.1
4,814,547 + +1.8

Or see this region's nucleotide sequence