Experiment: R2A control with 0.2% methanol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4238 and PP_5665 are separated by 392 nucleotides PP_5665 and PP_5666 are separated by 58 nucleotides PP_5666 and PP_5667 overlap by 1 nucleotides PP_5667 and PP_4240 are separated by 4 nucleotides
PP_4238: PP_4238 - conserved protein of unknown function, at 4,811,757 to 4,812,080
_4238
PP_5665: PP_5665 - putative microcin B17-like protein, McbA family, at 4,812,473 to 4,812,715
_5665
PP_5666: PP_5666 - putative microcin B17-like processing protein, McbB family, at 4,812,774 to 4,813,637
_5666
PP_5667: PP_5667 - putative microcin B17-like-processing protein, McbC family, at 4,813,637 to 4,814,428
_5667
PP_4240: PP_4240 - putative Microcin b17 processing protein mcbd, at 4,814,433 to 4,815,635
_4240
Position (kb)
4812
4813
4814 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4811.995 kb on + strand, within PP_4238 at 4812.017 kb on + strand, within PP_4238 at 4812.017 kb on + strand, within PP_4238 at 4812.078 kb on + strand at 4812.131 kb on + strand at 4812.150 kb on - strand at 4812.230 kb on + strand at 4812.452 kb on + strand at 4812.453 kb on - strand at 4812.523 kb on + strand, within PP_5665 at 4812.692 kb on - strand at 4813.050 kb on + strand, within PP_5666 at 4813.149 kb on - strand, within PP_5666 at 4813.168 kb on + strand, within PP_5666 at 4813.229 kb on - strand, within PP_5666 at 4813.353 kb on - strand, within PP_5666 at 4813.475 kb on - strand, within PP_5666 at 4813.632 kb on - strand at 4813.721 kb on + strand, within PP_5667 at 4813.721 kb on + strand, within PP_5667 at 4813.962 kb on + strand, within PP_5667 at 4814.203 kb on + strand, within PP_5667 at 4814.207 kb on + strand, within PP_5667 at 4814.241 kb on - strand at 4814.288 kb on + strand, within PP_5667 at 4814.349 kb on - strand at 4814.350 kb on + strand at 4814.486 kb on + strand at 4814.547 kb on + strand at 4814.547 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A control with 0.2% methanol remove 4,811,995 + PP_4238 0.73 +1.1 4,812,017 + PP_4238 0.80 -3.9 4,812,017 + PP_4238 0.80 -0.2 4,812,078 + +0.9 4,812,131 + -1.2 4,812,150 - +0.3 4,812,230 + -2.4 4,812,452 + -3.0 4,812,453 - +0.8 4,812,523 + PP_5665 0.21 -0.3 4,812,692 - +0.0 4,813,050 + PP_5666 0.32 -2.3 4,813,149 - PP_5666 0.43 -0.1 4,813,168 + PP_5666 0.46 -0.4 4,813,229 - PP_5666 0.53 -1.4 4,813,353 - PP_5666 0.67 -1.0 4,813,475 - PP_5666 0.81 -0.4 4,813,632 - -2.6 4,813,721 + PP_5667 0.11 -1.4 4,813,721 + PP_5667 0.11 +0.8 4,813,962 + PP_5667 0.41 -1.9 4,814,203 + PP_5667 0.71 -1.1 4,814,207 + PP_5667 0.72 -1.2 4,814,241 - +0.1 4,814,288 + PP_5667 0.82 +0.1 4,814,349 - -1.7 4,814,350 + -0.7 4,814,486 + -1.5 4,814,547 + -0.1 4,814,547 + +1.8
Or see this region's nucleotide sequence