Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0385 and PP_5445 are separated by 343 nucleotides PP_5445 and PP_t71 are separated by 52 nucleotides PP_t71 and PP_0386 are separated by 16 nucleotides
PP_0385: PP_0385 - putative serine acetyltransferase, at 465,999 to 466,553
_0385
PP_5445: PP_5445 - conserved protein of unknown function, at 466,897 to 467,067
_5445
PP_t71: PP_t71 - tRNA-Met, at 467,120 to 467,196
_t71
PP_0386: PP_0386 - Sensory box protein, at 467,213 to 470,956
_0386
Position (kb)
466
467
468 Strain fitness (log2 ratio)
-2
-1
0
1 at 466.067 kb on + strand, within PP_0385 at 466.149 kb on + strand, within PP_0385 at 466.149 kb on + strand, within PP_0385 at 466.218 kb on + strand, within PP_0385 at 466.299 kb on + strand, within PP_0385 at 466.300 kb on - strand, within PP_0385 at 466.374 kb on + strand, within PP_0385 at 466.375 kb on - strand, within PP_0385 at 466.456 kb on + strand, within PP_0385 at 466.457 kb on - strand, within PP_0385 at 466.512 kb on + strand at 466.564 kb on + strand at 466.565 kb on - strand at 466.565 kb on - strand at 466.616 kb on + strand at 466.616 kb on + strand at 466.688 kb on - strand at 466.728 kb on - strand at 466.756 kb on + strand at 466.865 kb on + strand at 466.865 kb on + strand at 466.903 kb on + strand at 466.904 kb on - strand at 466.904 kb on - strand at 466.922 kb on + strand, within PP_5445 at 466.923 kb on - strand, within PP_5445 at 467.006 kb on - strand, within PP_5445 at 467.051 kb on + strand at 467.051 kb on + strand at 467.052 kb on - strand at 467.070 kb on - strand at 467.202 kb on - strand at 467.202 kb on - strand at 467.215 kb on - strand at 467.215 kb on - strand at 467.217 kb on - strand at 467.262 kb on - strand at 467.262 kb on - strand at 467.262 kb on - strand at 467.317 kb on - strand at 467.317 kb on - strand at 467.317 kb on - strand at 467.520 kb on + strand at 467.521 kb on - strand at 467.521 kb on - strand at 467.567 kb on + strand at 467.589 kb on + strand, within PP_0386 at 467.589 kb on + strand, within PP_0386 at 467.590 kb on - strand, within PP_0386 at 467.766 kb on + strand, within PP_0386 at 467.766 kb on + strand, within PP_0386 at 467.767 kb on - strand, within PP_0386 at 467.810 kb on + strand, within PP_0386
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 466,067 + PP_0385 0.12 +0.7 466,149 + PP_0385 0.27 -0.4 466,149 + PP_0385 0.27 +0.2 466,218 + PP_0385 0.39 +0.1 466,299 + PP_0385 0.54 +0.1 466,300 - PP_0385 0.54 +0.5 466,374 + PP_0385 0.68 +1.0 466,375 - PP_0385 0.68 -0.2 466,456 + PP_0385 0.82 +0.2 466,457 - PP_0385 0.83 +0.5 466,512 + -1.1 466,564 + -1.1 466,565 - -1.0 466,565 - +0.3 466,616 + -0.6 466,616 + -1.7 466,688 - +0.3 466,728 - +0.2 466,756 + -1.0 466,865 + +0.3 466,865 + -1.0 466,903 + -1.2 466,904 - -0.5 466,904 - +0.8 466,922 + PP_5445 0.15 -0.8 466,923 - PP_5445 0.15 -1.0 467,006 - PP_5445 0.64 -2.0 467,051 + +0.1 467,051 + +0.3 467,052 - +0.0 467,070 - -0.5 467,202 - -1.9 467,202 - -0.1 467,215 - +0.8 467,215 - -0.1 467,217 - -0.1 467,262 - -0.2 467,262 - +0.4 467,262 - +0.6 467,317 - +1.2 467,317 - -1.0 467,317 - +0.5 467,520 + +0.1 467,521 - +0.3 467,521 - +0.2 467,567 + +0.6 467,589 + PP_0386 0.10 -1.0 467,589 + PP_0386 0.10 -1.1 467,590 - PP_0386 0.10 -1.2 467,766 + PP_0386 0.15 -0.1 467,766 + PP_0386 0.15 -0.5 467,767 - PP_0386 0.15 +1.6 467,810 + PP_0386 0.16 -0.1
Or see this region's nucleotide sequence