Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4813 and PP_4814 are separated by 111 nucleotides PP_4814 and PP_4815 are separated by 6 nucleotides PP_4815 and PP_4816 overlap by 4 nucleotides
PP_4813: PP_4813 - PAP2 family protein/DedA family protein, at 5,475,771 to 5,477,087
_4813
PP_4814: PP_4814 - ATP-dependent protease La domain protein, at 5,477,199 to 5,477,792
_4814
PP_4815: PP_4815 - putative Membrane protein, at 5,477,799 to 5,478,515
_4815
PP_4816: PP_4816 - conserved protein of unknown function, at 5,478,512 to 5,478,880
_4816
Position (kb) 5477
5478 Strain fitness (log2 ratio) -2
-1
0
1 at 5476.243 kb on - strand, within PP_4813 at 5476.243 kb on - strand, within PP_4813 at 5476.322 kb on - strand, within PP_4813 at 5476.322 kb on - strand, within PP_4813 at 5476.433 kb on - strand, within PP_4813 at 5476.433 kb on - strand, within PP_4813 at 5476.449 kb on + strand, within PP_4813 at 5476.449 kb on + strand, within PP_4813 at 5476.450 kb on - strand, within PP_4813 at 5476.450 kb on - strand, within PP_4813 at 5476.678 kb on + strand, within PP_4813 at 5476.678 kb on + strand, within PP_4813 at 5476.679 kb on - strand, within PP_4813 at 5476.703 kb on - strand, within PP_4813 at 5476.725 kb on + strand, within PP_4813 at 5476.725 kb on + strand, within PP_4813 at 5476.726 kb on - strand, within PP_4813 at 5476.726 kb on - strand, within PP_4813 at 5477.076 kb on + strand at 5477.076 kb on + strand at 5477.077 kb on - strand at 5477.077 kb on - strand at 5477.077 kb on - strand at 5477.082 kb on + strand at 5477.083 kb on - strand at 5477.083 kb on - strand at 5477.083 kb on - strand at 5477.106 kb on - strand at 5477.116 kb on - strand at 5477.128 kb on - strand at 5477.145 kb on + strand at 5477.151 kb on - strand at 5477.151 kb on - strand at 5477.190 kb on - strand at 5477.190 kb on - strand at 5477.317 kb on + strand, within PP_4814 at 5477.418 kb on + strand, within PP_4814 at 5477.419 kb on - strand, within PP_4814 at 5477.419 kb on - strand, within PP_4814 at 5477.762 kb on + strand at 5477.841 kb on - strand at 5478.023 kb on + strand, within PP_4815 at 5478.204 kb on - strand, within PP_4815 at 5478.204 kb on - strand, within PP_4815 at 5478.207 kb on - strand, within PP_4815 at 5478.207 kb on - strand, within PP_4815 at 5478.212 kb on + strand, within PP_4815 at 5478.213 kb on - strand, within PP_4815 at 5478.245 kb on + strand, within PP_4815 at 5478.250 kb on + strand, within PP_4815 at 5478.251 kb on - strand, within PP_4815 at 5478.375 kb on + strand, within PP_4815 at 5478.381 kb on + strand, within PP_4815 at 5478.416 kb on - strand, within PP_4815 at 5478.429 kb on + strand, within PP_4815 at 5478.429 kb on + strand, within PP_4815 at 5478.430 kb on - strand, within PP_4815 at 5478.436 kb on + strand, within PP_4815 at 5478.436 kb on + strand, within PP_4815 at 5478.648 kb on + strand, within PP_4816 at 5478.648 kb on + strand, within PP_4816 at 5478.648 kb on + strand, within PP_4816 at 5478.648 kb on + strand, within PP_4816 at 5478.649 kb on - strand, within PP_4816 at 5478.649 kb on - strand, within PP_4816 at 5478.649 kb on - strand, within PP_4816 at 5478.650 kb on + strand, within PP_4816 at 5478.650 kb on + strand, within PP_4816 at 5478.650 kb on + strand, within PP_4816 at 5478.650 kb on + strand, within PP_4816 at 5478.650 kb on + strand, within PP_4816 at 5478.650 kb on + strand, within PP_4816 at 5478.651 kb on - strand, within PP_4816 at 5478.651 kb on - strand, within PP_4816 at 5478.651 kb on - strand, within PP_4816
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 5,476,243 - PP_4813 0.36 -2.7 5,476,243 - PP_4813 0.36 -0.1 5,476,322 - PP_4813 0.42 +0.5 5,476,322 - PP_4813 0.42 +0.1 5,476,433 - PP_4813 0.50 -1.4 5,476,433 - PP_4813 0.50 +0.4 5,476,449 + PP_4813 0.51 -1.8 5,476,449 + PP_4813 0.51 +0.7 5,476,450 - PP_4813 0.52 +0.4 5,476,450 - PP_4813 0.52 -2.0 5,476,678 + PP_4813 0.69 -0.2 5,476,678 + PP_4813 0.69 -0.2 5,476,679 - PP_4813 0.69 -0.4 5,476,703 - PP_4813 0.71 -1.4 5,476,725 + PP_4813 0.72 +0.4 5,476,725 + PP_4813 0.72 -0.8 5,476,726 - PP_4813 0.73 +0.3 5,476,726 - PP_4813 0.73 +0.1 5,477,076 + -0.0 5,477,076 + +0.9 5,477,077 - -2.1 5,477,077 - +0.3 5,477,077 - +0.6 5,477,082 + -0.0 5,477,083 - -0.2 5,477,083 - +0.2 5,477,083 - +0.8 5,477,106 - -1.6 5,477,116 - +0.7 5,477,128 - -2.2 5,477,145 + +0.1 5,477,151 - +0.5 5,477,151 - +0.5 5,477,190 - -0.1 5,477,190 - +0.1 5,477,317 + PP_4814 0.20 +1.3 5,477,418 + PP_4814 0.37 +0.5 5,477,419 - PP_4814 0.37 +0.5 5,477,419 - PP_4814 0.37 +1.1 5,477,762 + +0.0 5,477,841 - +0.2 5,478,023 + PP_4815 0.31 -0.6 5,478,204 - PP_4815 0.56 +0.8 5,478,204 - PP_4815 0.56 -0.6 5,478,207 - PP_4815 0.57 -0.6 5,478,207 - PP_4815 0.57 +1.4 5,478,212 + PP_4815 0.58 +0.5 5,478,213 - PP_4815 0.58 -0.4 5,478,245 + PP_4815 0.62 +0.5 5,478,250 + PP_4815 0.63 -0.4 5,478,251 - PP_4815 0.63 -0.6 5,478,375 + PP_4815 0.80 -0.2 5,478,381 + PP_4815 0.81 -0.9 5,478,416 - PP_4815 0.86 +0.4 5,478,429 + PP_4815 0.88 +0.2 5,478,429 + PP_4815 0.88 +0.2 5,478,430 - PP_4815 0.88 +0.4 5,478,436 + PP_4815 0.89 +0.1 5,478,436 + PP_4815 0.89 +0.2 5,478,648 + PP_4816 0.37 -0.4 5,478,648 + PP_4816 0.37 +0.4 5,478,648 + PP_4816 0.37 -0.3 5,478,648 + PP_4816 0.37 +0.2 5,478,649 - PP_4816 0.37 -0.1 5,478,649 - PP_4816 0.37 +0.6 5,478,649 - PP_4816 0.37 +0.1 5,478,650 + PP_4816 0.37 -0.4 5,478,650 + PP_4816 0.37 -0.2 5,478,650 + PP_4816 0.37 +1.5 5,478,650 + PP_4816 0.37 -1.0 5,478,650 + PP_4816 0.37 -1.5 5,478,650 + PP_4816 0.37 +0.4 5,478,651 - PP_4816 0.38 -0.0 5,478,651 - PP_4816 0.38 -1.0 5,478,651 - PP_4816 0.38 +1.3
Or see this region's nucleotide sequence