Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2623 and PP_2624 are separated by 17 nucleotides PP_2624 and PP_2625 are separated by 281 nucleotides PP_2625 and PP_2626 overlap by 13 nucleotides
PP_2623: PP_2623 - conserved protein of unknown function, at 2,998,984 to 3,000,474
_2623
PP_2624: PP_2624 - conserved protein of unknown function, at 3,000,492 to 3,000,995
_2624
PP_2625: PP_2625 - conserved exported protein of unknown function, at 3,001,277 to 3,001,972
_2625
PP_2626: PP_2626 - conserved protein of unknown function, at 3,001,960 to 3,003,423
_2626
Position (kb)
3000
3001 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2999.648 kb on + strand, within PP_2623 at 2999.649 kb on - strand, within PP_2623 at 2999.681 kb on + strand, within PP_2623 at 2999.681 kb on + strand, within PP_2623 at 2999.842 kb on - strand, within PP_2623 at 2999.934 kb on - strand, within PP_2623 at 2999.939 kb on - strand, within PP_2623 at 2999.976 kb on - strand, within PP_2623 at 3000.044 kb on + strand, within PP_2623 at 3000.044 kb on + strand, within PP_2623 at 3000.334 kb on + strand at 3000.373 kb on + strand at 3000.373 kb on + strand at 3000.373 kb on + strand at 3000.374 kb on - strand at 3000.415 kb on - strand at 3000.563 kb on + strand, within PP_2624 at 3000.564 kb on - strand, within PP_2624 at 3000.564 kb on - strand, within PP_2624 at 3000.815 kb on + strand, within PP_2624 at 3000.815 kb on + strand, within PP_2624 at 3000.815 kb on + strand, within PP_2624 at 3000.816 kb on - strand, within PP_2624 at 3000.816 kb on - strand at 3001.051 kb on - strand at 3001.083 kb on - strand at 3001.200 kb on + strand at 3001.286 kb on - strand at 3001.338 kb on + strand at 3001.338 kb on + strand at 3001.338 kb on + strand at 3001.339 kb on - strand at 3001.340 kb on + strand at 3001.470 kb on - strand, within PP_2625 at 3001.658 kb on + strand, within PP_2625 at 3001.658 kb on + strand, within PP_2625 at 3001.658 kb on + strand, within PP_2625 at 3001.659 kb on - strand, within PP_2625 at 3001.659 kb on - strand, within PP_2625 at 3001.677 kb on + strand, within PP_2625 at 3001.677 kb on + strand, within PP_2625 at 3001.677 kb on + strand, within PP_2625 at 3001.678 kb on - strand at 3001.740 kb on + strand, within PP_2625 at 3001.741 kb on - strand, within PP_2625
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 2,999,648 + PP_2623 0.45 -0.7 2,999,649 - PP_2623 0.45 +0.1 2,999,681 + PP_2623 0.47 +0.4 2,999,681 + PP_2623 0.47 +0.0 2,999,842 - PP_2623 0.58 +0.6 2,999,934 - PP_2623 0.64 +0.2 2,999,939 - PP_2623 0.64 -1.3 2,999,976 - PP_2623 0.67 +2.5 3,000,044 + PP_2623 0.71 +1.2 3,000,044 + PP_2623 0.71 +0.3 3,000,334 + +1.6 3,000,373 + +0.4 3,000,373 + +0.4 3,000,373 + -0.4 3,000,374 - +1.3 3,000,415 - +0.6 3,000,563 + PP_2624 0.14 +0.5 3,000,564 - PP_2624 0.14 -1.6 3,000,564 - PP_2624 0.14 -1.7 3,000,815 + PP_2624 0.64 -2.8 3,000,815 + PP_2624 0.64 -1.1 3,000,815 + PP_2624 0.64 -0.3 3,000,816 - PP_2624 0.64 -1.5 3,000,816 - -0.1 3,001,051 - -0.5 3,001,083 - -2.1 3,001,200 + -1.2 3,001,286 - -1.1 3,001,338 + +0.2 3,001,338 + +0.4 3,001,338 + +0.2 3,001,339 - -0.4 3,001,340 + -0.0 3,001,470 - PP_2625 0.28 +0.5 3,001,658 + PP_2625 0.55 +0.1 3,001,658 + PP_2625 0.55 +0.6 3,001,658 + PP_2625 0.55 +0.1 3,001,659 - PP_2625 0.55 +0.5 3,001,659 - PP_2625 0.55 -0.6 3,001,677 + PP_2625 0.57 -0.1 3,001,677 + PP_2625 0.57 -0.8 3,001,677 + PP_2625 0.57 +0.2 3,001,678 - +0.7 3,001,740 + PP_2625 0.67 +1.2 3,001,741 - PP_2625 0.67 +0.7
Or see this region's nucleotide sequence