Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2158 and PP_2159 are separated by 200 nucleotides PP_2159 and PP_2160 are separated by 68 nucleotides PP_2160 and PP_2161 are separated by 31 nucleotides PP_2161 and PP_2162 are separated by 122 nucleotides
PP_2158: PP_2158 - transcriptional activator protein, at 2,465,634 to 2,466,314
_2158
PP_2159: PP_2159 - Copper-binding protein, plastocyanin/azurin family, at 2,466,515 to 2,467,042
_2159
PP_2160: PP_2160 - NADPH-dependent 7-cyano-7-deazaguanine reductase, at 2,467,111 to 2,467,941
_2160
PP_2161: PP_2161 - conserved protein of unknown function, at 2,467,973 to 2,468,236
_2161
PP_2162: PP_2162 - putative phosphonoacetaldehyde phosphonohydrolase-related protein, at 2,468,359 to 2,468,922
_2162
Position (kb)
2467
2468 Strain fitness (log2 ratio)
-2
-1
0
1 at 2466.272 kb on - strand at 2466.325 kb on + strand at 2466.344 kb on + strand at 2466.345 kb on - strand at 2466.345 kb on - strand at 2466.399 kb on + strand at 2466.439 kb on + strand at 2466.455 kb on + strand at 2466.456 kb on - strand at 2466.477 kb on - strand at 2466.500 kb on - strand at 2466.628 kb on + strand, within PP_2159 at 2466.628 kb on + strand, within PP_2159 at 2466.681 kb on + strand, within PP_2159 at 2466.835 kb on + strand, within PP_2159 at 2467.005 kb on - strand at 2467.078 kb on + strand at 2467.078 kb on + strand at 2467.098 kb on + strand at 2467.099 kb on - strand at 2467.156 kb on + strand at 2467.157 kb on - strand at 2467.168 kb on + strand at 2467.168 kb on + strand at 2467.169 kb on - strand at 2467.262 kb on - strand, within PP_2160 at 2467.262 kb on - strand, within PP_2160 at 2467.262 kb on - strand, within PP_2160 at 2467.433 kb on + strand, within PP_2160 at 2467.433 kb on + strand, within PP_2160 at 2467.433 kb on + strand, within PP_2160 at 2467.434 kb on - strand, within PP_2160 at 2467.434 kb on - strand, within PP_2160 at 2467.573 kb on + strand, within PP_2160 at 2467.573 kb on + strand, within PP_2160 at 2467.706 kb on - strand, within PP_2160 at 2467.745 kb on - strand, within PP_2160 at 2467.855 kb on + strand, within PP_2160 at 2467.856 kb on - strand, within PP_2160 at 2467.943 kb on - strand at 2467.943 kb on - strand at 2468.175 kb on - strand, within PP_2161 at 2468.312 kb on + strand at 2468.357 kb on + strand at 2468.358 kb on - strand at 2468.358 kb on - strand at 2468.358 kb on - strand at 2468.623 kb on + strand, within PP_2162 at 2468.894 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 2,466,272 - -0.3 2,466,325 + -0.9 2,466,344 + +0.1 2,466,345 - -0.6 2,466,345 - -0.5 2,466,399 + -0.3 2,466,439 + -0.0 2,466,455 + -1.7 2,466,456 - +0.0 2,466,477 - -0.1 2,466,500 - -0.5 2,466,628 + PP_2159 0.21 -0.1 2,466,628 + PP_2159 0.21 -1.1 2,466,681 + PP_2159 0.31 -1.2 2,466,835 + PP_2159 0.61 +0.2 2,467,005 - +0.2 2,467,078 + -0.8 2,467,078 + +0.2 2,467,098 + -0.3 2,467,099 - +0.4 2,467,156 + +0.8 2,467,157 - +0.8 2,467,168 + +0.6 2,467,168 + -0.8 2,467,169 - -0.0 2,467,262 - PP_2160 0.18 -2.0 2,467,262 - PP_2160 0.18 +1.2 2,467,262 - PP_2160 0.18 -0.3 2,467,433 + PP_2160 0.39 -0.6 2,467,433 + PP_2160 0.39 +0.2 2,467,433 + PP_2160 0.39 +0.1 2,467,434 - PP_2160 0.39 -0.7 2,467,434 - PP_2160 0.39 -0.1 2,467,573 + PP_2160 0.56 +0.6 2,467,573 + PP_2160 0.56 -0.9 2,467,706 - PP_2160 0.72 +1.5 2,467,745 - PP_2160 0.76 -1.6 2,467,855 + PP_2160 0.90 +1.6 2,467,856 - PP_2160 0.90 -0.1 2,467,943 - -0.7 2,467,943 - +1.3 2,468,175 - PP_2161 0.77 +0.0 2,468,312 + +0.3 2,468,357 + +0.4 2,468,358 - -0.0 2,468,358 - -1.2 2,468,358 - -0.1 2,468,623 + PP_2162 0.47 +0.7 2,468,894 - -1.0
Or see this region's nucleotide sequence