Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2089 and PP_2090 are separated by 86 nucleotides PP_2090 and PP_2091 are separated by 27 nucleotides
PP_2089: PP_2089 - Outer membrane porin F, at 2,380,337 to 2,381,371
_2089
PP_2090: PP_2090 - Uroporphyrinogen-III C-methyltransferase, at 2,381,458 to 2,382,198
_2090
PP_2091: PP_2091 - putative Serine/threonine-protein kinase, at 2,382,226 to 2,383,896
_2091
Position (kb)
2381
2382
2383 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2380.514 kb on + strand, within PP_2089 at 2380.985 kb on - strand, within PP_2089 at 2381.123 kb on + strand, within PP_2089 at 2381.240 kb on + strand, within PP_2089 at 2381.247 kb on + strand at 2381.248 kb on - strand, within PP_2089 at 2381.353 kb on - strand at 2381.353 kb on - strand at 2381.369 kb on + strand at 2381.369 kb on + strand at 2381.369 kb on + strand at 2381.370 kb on - strand at 2381.370 kb on - strand at 2381.370 kb on - strand at 2381.430 kb on + strand at 2381.484 kb on + strand at 2381.699 kb on + strand, within PP_2090 at 2381.699 kb on + strand, within PP_2090 at 2381.700 kb on - strand, within PP_2090 at 2381.751 kb on + strand, within PP_2090 at 2382.097 kb on + strand, within PP_2090 at 2382.184 kb on + strand at 2382.185 kb on - strand at 2382.185 kb on - strand at 2382.185 kb on - strand at 2382.253 kb on + strand at 2382.254 kb on - strand at 2382.254 kb on - strand at 2382.296 kb on - strand at 2382.517 kb on + strand, within PP_2091 at 2382.575 kb on + strand, within PP_2091 at 2382.575 kb on + strand, within PP_2091 at 2382.575 kb on + strand, within PP_2091 at 2382.575 kb on + strand, within PP_2091 at 2382.576 kb on - strand, within PP_2091 at 2382.576 kb on - strand, within PP_2091 at 2382.576 kb on - strand, within PP_2091 at 2382.629 kb on + strand, within PP_2091 at 2382.629 kb on + strand, within PP_2091 at 2382.629 kb on + strand, within PP_2091 at 2382.629 kb on + strand, within PP_2091 at 2382.629 kb on + strand, within PP_2091 at 2382.629 kb on + strand, within PP_2091 at 2382.629 kb on + strand, within PP_2091 at 2382.629 kb on + strand, within PP_2091 at 2382.630 kb on - strand, within PP_2091 at 2382.630 kb on - strand, within PP_2091 at 2382.630 kb on - strand, within PP_2091 at 2382.897 kb on - strand, within PP_2091 at 2382.897 kb on - strand, within PP_2091 at 2382.899 kb on + strand, within PP_2091 at 2382.900 kb on - strand, within PP_2091 at 2382.900 kb on - strand, within PP_2091 at 2382.900 kb on - strand, within PP_2091 at 2382.900 kb on - strand, within PP_2091 at 2382.928 kb on + strand, within PP_2091 at 2382.929 kb on - strand at 2383.071 kb on - strand, within PP_2091
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 2,380,514 + PP_2089 0.17 +1.7 2,380,985 - PP_2089 0.63 -2.7 2,381,123 + PP_2089 0.76 -0.6 2,381,240 + PP_2089 0.87 -2.3 2,381,247 + -3.1 2,381,248 - PP_2089 0.88 -2.6 2,381,353 - -0.8 2,381,353 - -2.3 2,381,369 + -3.7 2,381,369 + -3.3 2,381,369 + -2.9 2,381,370 - -3.8 2,381,370 - -1.1 2,381,370 - -3.0 2,381,430 + -0.3 2,381,484 + -1.6 2,381,699 + PP_2090 0.33 -0.9 2,381,699 + PP_2090 0.33 -0.9 2,381,700 - PP_2090 0.33 -0.6 2,381,751 + PP_2090 0.40 -1.9 2,382,097 + PP_2090 0.86 -1.0 2,382,184 + -2.2 2,382,185 - +0.4 2,382,185 - -0.3 2,382,185 - -1.0 2,382,253 + -1.2 2,382,254 - -0.5 2,382,254 - +0.6 2,382,296 - -2.6 2,382,517 + PP_2091 0.17 -1.3 2,382,575 + PP_2091 0.21 -1.0 2,382,575 + PP_2091 0.21 +0.2 2,382,575 + PP_2091 0.21 -0.4 2,382,575 + PP_2091 0.21 +0.1 2,382,576 - PP_2091 0.21 -0.6 2,382,576 - PP_2091 0.21 -0.6 2,382,576 - PP_2091 0.21 -0.5 2,382,629 + PP_2091 0.24 -0.1 2,382,629 + PP_2091 0.24 -0.8 2,382,629 + PP_2091 0.24 -0.9 2,382,629 + PP_2091 0.24 -2.8 2,382,629 + PP_2091 0.24 -2.0 2,382,629 + PP_2091 0.24 -3.0 2,382,629 + PP_2091 0.24 -1.1 2,382,629 + PP_2091 0.24 -1.1 2,382,630 - PP_2091 0.24 -2.3 2,382,630 - PP_2091 0.24 -1.8 2,382,630 - PP_2091 0.24 +0.3 2,382,897 - PP_2091 0.40 -0.0 2,382,897 - PP_2091 0.40 -1.0 2,382,899 + PP_2091 0.40 -0.3 2,382,900 - PP_2091 0.40 -1.1 2,382,900 - PP_2091 0.40 -0.4 2,382,900 - PP_2091 0.40 -0.5 2,382,900 - PP_2091 0.40 -1.1 2,382,928 + PP_2091 0.42 -0.7 2,382,929 - +0.5 2,383,071 - PP_2091 0.51 -0.6
Or see this region's nucleotide sequence