Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2069 and PP_2070 are separated by 100 nucleotides PP_2070 and PP_2071 are separated by 169 nucleotides PP_2071 and PP_2072 are separated by 108 nucleotides PP_2072 and PP_2073 are separated by 19 nucleotides
PP_2069: PP_2069 - putative Multidrug efflux MFS outer membrane protein, at 2,354,589 to 2,356,010
_2069
PP_2070: PP_2070 - Transcriptional regulator, AraC family, at 2,356,111 to 2,356,821
_2070
PP_2071: PP_2071 - conserved protein of unknown function, at 2,356,991 to 2,357,377
_2071
PP_2072: PP_2072 - Transcriptional regulator, AraC family, at 2,357,486 to 2,358,310
_2072
PP_2073: PP_2073 - Acetyltransferase, GNAT family, at 2,358,330 to 2,358,845
_2073
Position (kb)
2356
2357
2358 Strain fitness (log2 ratio)
-2
-1
0
1 at 2355.999 kb on + strand at 2355.999 kb on + strand at 2356.000 kb on - strand at 2356.000 kb on - strand at 2356.115 kb on + strand at 2356.116 kb on - strand at 2356.128 kb on + strand at 2356.183 kb on - strand, within PP_2070 at 2356.296 kb on - strand, within PP_2070 at 2356.850 kb on + strand at 2357.127 kb on + strand, within PP_2071 at 2357.127 kb on + strand, within PP_2071 at 2357.127 kb on + strand, within PP_2071 at 2357.127 kb on + strand, within PP_2071 at 2357.128 kb on - strand, within PP_2071 at 2357.128 kb on - strand, within PP_2071 at 2357.128 kb on - strand, within PP_2071 at 2357.128 kb on - strand, within PP_2071 at 2357.235 kb on - strand, within PP_2071 at 2357.269 kb on - strand, within PP_2071 at 2357.315 kb on + strand, within PP_2071 at 2357.409 kb on + strand at 2357.409 kb on + strand at 2357.436 kb on + strand at 2357.437 kb on - strand at 2357.519 kb on + strand at 2357.536 kb on - strand at 2357.682 kb on + strand, within PP_2072 at 2357.683 kb on - strand, within PP_2072 at 2357.753 kb on + strand, within PP_2072 at 2357.753 kb on + strand, within PP_2072 at 2357.753 kb on + strand, within PP_2072 at 2357.754 kb on - strand, within PP_2072 at 2357.809 kb on - strand, within PP_2072 at 2357.809 kb on - strand, within PP_2072 at 2357.871 kb on + strand, within PP_2072 at 2357.871 kb on + strand, within PP_2072 at 2357.871 kb on + strand, within PP_2072 at 2357.872 kb on - strand, within PP_2072 at 2357.921 kb on + strand, within PP_2072 at 2357.944 kb on - strand, within PP_2072 at 2358.030 kb on + strand, within PP_2072 at 2358.141 kb on + strand, within PP_2072 at 2358.141 kb on + strand, within PP_2072 at 2358.141 kb on + strand, within PP_2072 at 2358.141 kb on + strand, within PP_2072 at 2358.142 kb on - strand, within PP_2072 at 2358.170 kb on - strand, within PP_2072 at 2358.183 kb on + strand, within PP_2072 at 2358.184 kb on - strand, within PP_2072 at 2358.187 kb on + strand, within PP_2072 at 2358.188 kb on - strand, within PP_2072 at 2358.188 kb on - strand at 2358.262 kb on + strand at 2358.263 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 2,355,999 + +0.6 2,355,999 + -0.6 2,356,000 - -0.1 2,356,000 - +0.5 2,356,115 + -0.4 2,356,116 - +0.6 2,356,128 + +0.3 2,356,183 - PP_2070 0.10 +0.5 2,356,296 - PP_2070 0.26 +0.7 2,356,850 + -0.8 2,357,127 + PP_2071 0.35 +0.3 2,357,127 + PP_2071 0.35 -0.2 2,357,127 + PP_2071 0.35 +0.8 2,357,127 + PP_2071 0.35 +0.1 2,357,128 - PP_2071 0.35 +1.5 2,357,128 - PP_2071 0.35 +0.7 2,357,128 - PP_2071 0.35 +0.2 2,357,128 - PP_2071 0.35 -0.3 2,357,235 - PP_2071 0.63 -1.3 2,357,269 - PP_2071 0.72 -0.3 2,357,315 + PP_2071 0.84 +0.2 2,357,409 + +0.6 2,357,409 + +0.2 2,357,436 + +1.1 2,357,437 - +1.3 2,357,519 + +0.5 2,357,536 - -2.0 2,357,682 + PP_2072 0.24 -0.5 2,357,683 - PP_2072 0.24 +1.3 2,357,753 + PP_2072 0.32 +0.2 2,357,753 + PP_2072 0.32 -0.1 2,357,753 + PP_2072 0.32 -0.0 2,357,754 - PP_2072 0.32 +0.7 2,357,809 - PP_2072 0.39 +0.4 2,357,809 - PP_2072 0.39 +0.1 2,357,871 + PP_2072 0.47 -0.3 2,357,871 + PP_2072 0.47 -0.3 2,357,871 + PP_2072 0.47 +0.9 2,357,872 - PP_2072 0.47 +0.5 2,357,921 + PP_2072 0.53 +0.0 2,357,944 - PP_2072 0.56 +0.5 2,358,030 + PP_2072 0.66 +0.2 2,358,141 + PP_2072 0.79 +0.9 2,358,141 + PP_2072 0.79 -1.5 2,358,141 + PP_2072 0.79 -0.2 2,358,141 + PP_2072 0.79 -0.5 2,358,142 - PP_2072 0.80 -0.6 2,358,170 - PP_2072 0.83 -1.5 2,358,183 + PP_2072 0.84 +0.3 2,358,184 - PP_2072 0.85 +0.6 2,358,187 + PP_2072 0.85 +0.1 2,358,188 - PP_2072 0.85 +0.8 2,358,188 - +0.7 2,358,262 + -0.6 2,358,263 - +0.7
Or see this region's nucleotide sequence