Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0955 and PP_0956 are separated by 8 nucleotides PP_0956 and PP_0957 are separated by 12 nucleotides PP_0957 and PP_0958 are separated by 248 nucleotides
PP_0955: PP_0955 - conserved exported protein of unknown function, at 1,097,473 to 1,098,045
_0955
PP_0956: PP_0956 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, at 1,098,054 to 1,098,578
_0956
PP_0957: PP_0957 - D-arabinose 5-phosphate isomerase, at 1,098,591 to 1,099,565
_0957
PP_0958: PP_0958 - phospholipid ABC transporter ATP-binding subunit, at 1,099,814 to 1,100,623
_0958
Position (kb)
1098
1099
1100 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1097.607 kb on - strand, within PP_0955 at 1098.694 kb on - strand, within PP_0957 at 1098.786 kb on - strand, within PP_0957 at 1099.602 kb on - strand at 1099.649 kb on + strand at 1099.649 kb on + strand at 1099.718 kb on + strand at 1099.718 kb on + strand at 1099.719 kb on - strand at 1099.719 kb on - strand at 1099.720 kb on + strand at 1099.720 kb on + strand at 1099.720 kb on + strand at 1099.720 kb on + strand at 1099.720 kb on + strand at 1099.721 kb on - strand at 1099.721 kb on - strand at 1099.721 kb on - strand at 1099.721 kb on - strand at 1099.721 kb on - strand at 1099.721 kb on - strand at 1099.721 kb on - strand at 1099.721 kb on - strand at 1099.783 kb on + strand at 1099.783 kb on + strand at 1099.799 kb on + strand at 1099.800 kb on - strand at 1099.811 kb on + strand at 1099.812 kb on - strand at 1099.855 kb on - strand at 1100.007 kb on + strand, within PP_0958 at 1100.007 kb on + strand, within PP_0958 at 1100.008 kb on - strand, within PP_0958 at 1100.008 kb on - strand, within PP_0958 at 1100.008 kb on - strand, within PP_0958 at 1100.152 kb on + strand, within PP_0958 at 1100.153 kb on - strand, within PP_0958 at 1100.316 kb on - strand, within PP_0958 at 1100.453 kb on + strand, within PP_0958 at 1100.453 kb on + strand, within PP_0958 at 1100.454 kb on - strand, within PP_0958 at 1100.459 kb on + strand, within PP_0958 at 1100.459 kb on + strand, within PP_0958 at 1100.459 kb on + strand, within PP_0958 at 1100.459 kb on + strand, within PP_0958 at 1100.460 kb on - strand, within PP_0958 at 1100.460 kb on - strand, within PP_0958 at 1100.460 kb on - strand, within PP_0958 at 1100.460 kb on - strand, within PP_0958 at 1100.460 kb on - strand, within PP_0958 at 1100.460 kb on - strand, within PP_0958 at 1100.466 kb on + strand, within PP_0958 at 1100.466 kb on + strand, within PP_0958 at 1100.467 kb on - strand, within PP_0958 at 1100.467 kb on - strand, within PP_0958
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 1,097,607 - PP_0955 0.23 -2.9 1,098,694 - PP_0957 0.11 +0.0 1,098,786 - PP_0957 0.20 +1.7 1,099,602 - +0.3 1,099,649 + -0.2 1,099,649 + -0.9 1,099,718 + -0.7 1,099,718 + -0.2 1,099,719 - -2.7 1,099,719 - -2.8 1,099,720 + -1.5 1,099,720 + -0.8 1,099,720 + +0.1 1,099,720 + +0.8 1,099,720 + -0.3 1,099,721 - -2.5 1,099,721 - -2.4 1,099,721 - -2.8 1,099,721 - -3.0 1,099,721 - -2.8 1,099,721 - +0.0 1,099,721 - -2.9 1,099,721 - -0.9 1,099,783 + -0.1 1,099,783 + +0.6 1,099,799 + +0.3 1,099,800 - -2.8 1,099,811 + -0.7 1,099,812 - -2.8 1,099,855 - -1.9 1,100,007 + PP_0958 0.24 -1.5 1,100,007 + PP_0958 0.24 -1.7 1,100,008 - PP_0958 0.24 -2.6 1,100,008 - PP_0958 0.24 -2.2 1,100,008 - PP_0958 0.24 -3.2 1,100,152 + PP_0958 0.42 -2.1 1,100,153 - PP_0958 0.42 -3.6 1,100,316 - PP_0958 0.62 -1.6 1,100,453 + PP_0958 0.79 -1.8 1,100,453 + PP_0958 0.79 -2.5 1,100,454 - PP_0958 0.79 -1.0 1,100,459 + PP_0958 0.80 -1.4 1,100,459 + PP_0958 0.80 +0.1 1,100,459 + PP_0958 0.80 -2.4 1,100,459 + PP_0958 0.80 -2.3 1,100,460 - PP_0958 0.80 -3.1 1,100,460 - PP_0958 0.80 -3.4 1,100,460 - PP_0958 0.80 -2.5 1,100,460 - PP_0958 0.80 -0.7 1,100,460 - PP_0958 0.80 -3.4 1,100,460 - PP_0958 0.80 -3.2 1,100,466 + PP_0958 0.80 -1.7 1,100,466 + PP_0958 0.80 -2.5 1,100,467 - PP_0958 0.81 -3.1 1,100,467 - PP_0958 0.81 -3.3
Or see this region's nucleotide sequence