Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0546 and PP_0547 are separated by 66 nucleotides PP_0547 and PP_0548 overlap by 4 nucleotides PP_0548 and PP_0549 overlap by 4 nucleotides
PP_0546: PP_0546 - sigma-54 dependent transcriptional regulator, at 633,695 to 635,710
_0546
PP_0547: PP_0547 - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase, at 635,777 to 637,126
_0547
PP_0548: PP_0548 - putative phenylacrylic acid decarboxylase, padA1, at 637,123 to 637,752
_0548
PP_0549: PP_0549 - putative Lipoprotein, at 637,749 to 638,036
_0549
Position (kb) 635
636
637
638 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 634.864 kb on + strand, within PP_0546 at 634.865 kb on - strand, within PP_0546 at 634.865 kb on - strand, within PP_0546 at 635.000 kb on + strand, within PP_0546 at 635.000 kb on - strand, within PP_0546 at 635.104 kb on + strand, within PP_0546 at 635.159 kb on + strand, within PP_0546 at 635.159 kb on + strand, within PP_0546 at 635.160 kb on - strand, within PP_0546 at 635.160 kb on - strand, within PP_0546 at 635.160 kb on - strand, within PP_0546 at 635.160 kb on - strand, within PP_0546 at 635.160 kb on - strand, within PP_0546 at 635.160 kb on - strand, within PP_0546 at 635.162 kb on + strand, within PP_0546 at 635.268 kb on + strand, within PP_0546 at 635.269 kb on - strand, within PP_0546 at 635.269 kb on - strand, within PP_0546 at 635.269 kb on - strand, within PP_0546 at 635.308 kb on + strand, within PP_0546 at 635.383 kb on + strand, within PP_0546 at 635.383 kb on + strand, within PP_0546 at 635.662 kb on + strand at 635.663 kb on - strand at 635.663 kb on - strand at 635.696 kb on - strand at 635.782 kb on + strand at 635.782 kb on + strand at 635.782 kb on + strand at 635.879 kb on + strand at 636.014 kb on + strand, within PP_0547 at 636.038 kb on + strand, within PP_0547 at 636.038 kb on + strand, within PP_0547 at 636.038 kb on + strand, within PP_0547 at 636.657 kb on + strand, within PP_0547 at 636.657 kb on + strand, within PP_0547 at 636.657 kb on + strand, within PP_0547 at 636.738 kb on + strand, within PP_0547 at 636.755 kb on + strand, within PP_0547 at 636.755 kb on + strand, within PP_0547 at 636.755 kb on + strand, within PP_0547 at 636.755 kb on + strand, within PP_0547 at 636.932 kb on + strand, within PP_0547 at 636.932 kb on + strand, within PP_0547 at 637.849 kb on - strand, within PP_0549 at 637.872 kb on + strand, within PP_0549 at 637.872 kb on + strand, within PP_0549 at 637.872 kb on + strand, within PP_0549 at 637.872 kb on + strand, within PP_0549 at 637.872 kb on + strand, within PP_0549 at 637.873 kb on - strand, within PP_0549 at 637.873 kb on - strand, within PP_0549 at 637.908 kb on + strand, within PP_0549 at 638.008 kb on + strand at 638.009 kb on - strand at 638.009 kb on - strand at 638.064 kb on + strand at 638.065 kb on - strand at 638.065 kb on - strand at 638.074 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 634,864 + PP_0546 0.58 -0.2 634,865 - PP_0546 0.58 +1.0 634,865 - PP_0546 0.58 -0.4 635,000 + PP_0546 0.65 -0.3 635,000 - PP_0546 0.65 -0.2 635,104 + PP_0546 0.70 -0.2 635,159 + PP_0546 0.73 -1.3 635,159 + PP_0546 0.73 -0.1 635,160 - PP_0546 0.73 -0.2 635,160 - PP_0546 0.73 +0.0 635,160 - PP_0546 0.73 +0.1 635,160 - PP_0546 0.73 -0.9 635,160 - PP_0546 0.73 -0.6 635,160 - PP_0546 0.73 +0.9 635,162 + PP_0546 0.73 -0.4 635,268 + PP_0546 0.78 +0.4 635,269 - PP_0546 0.78 -0.9 635,269 - PP_0546 0.78 +0.4 635,269 - PP_0546 0.78 +0.6 635,308 + PP_0546 0.80 -0.5 635,383 + PP_0546 0.84 +0.6 635,383 + PP_0546 0.84 -0.2 635,662 + +0.4 635,663 - -1.0 635,663 - -0.3 635,696 - +0.4 635,782 + -1.7 635,782 + -1.8 635,782 + -3.5 635,879 + -0.8 636,014 + PP_0547 0.18 -1.8 636,038 + PP_0547 0.19 -1.8 636,038 + PP_0547 0.19 -3.5 636,038 + PP_0547 0.19 -1.5 636,657 + PP_0547 0.65 +0.7 636,657 + PP_0547 0.65 -0.4 636,657 + PP_0547 0.65 -1.8 636,738 + PP_0547 0.71 -2.3 636,755 + PP_0547 0.72 -2.7 636,755 + PP_0547 0.72 -0.1 636,755 + PP_0547 0.72 -1.1 636,755 + PP_0547 0.72 -1.5 636,932 + PP_0547 0.86 -2.1 636,932 + PP_0547 0.86 -1.7 637,849 - PP_0549 0.35 -0.9 637,872 + PP_0549 0.43 -1.1 637,872 + PP_0549 0.43 -0.7 637,872 + PP_0549 0.43 -0.0 637,872 + PP_0549 0.43 -1.1 637,872 + PP_0549 0.43 -0.9 637,873 - PP_0549 0.43 +0.4 637,873 - PP_0549 0.43 -0.1 637,908 + PP_0549 0.55 +0.1 638,008 + +0.8 638,009 - +0.7 638,009 - +0.0 638,064 + +0.0 638,065 - +0.2 638,065 - +0.7 638,074 + -0.4
Or see this region's nucleotide sequence