Strain Fitness in Pseudomonas putida KT2440 around PP_0188

Experiment: R2A with Fusaric 480 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0186 and PP_0187 overlap by 4 nucleotidesPP_0187 and PP_0188 are separated by 18 nucleotidesPP_0188 and PP_0189 overlap by 4 nucleotides PP_0186: PP_0186 - Porphobilinogen deaminase, at 237,982 to 238,923 _0186 PP_0187: PP_0187 - Uroporphyrinogen-III synthase, at 238,920 to 239,687 _0187 PP_0188: PP_0188 - putative Uroporphyrin-III C-methyltransferase, at 239,706 to 240,833 _0188 PP_0189: PP_0189 - putative heme biosynthesis protein, at 240,830 to 242,068 _0189 Position (kb) 239 240 241Strain fitness (log2 ratio) -2 -1 0 1 2at 239.685 kb on + strandat 239.685 kb on + strandat 239.685 kb on + strandat 239.685 kb on + strandat 239.685 kb on + strandat 239.686 kb on - strandat 239.693 kb on + strandat 239.694 kb on - strandat 239.694 kb on - strandat 239.730 kb on + strandat 239.730 kb on + strandat 239.731 kb on - strandat 239.731 kb on - strandat 239.731 kb on - strandat 239.762 kb on + strandat 239.763 kb on - strandat 239.763 kb on - strandat 239.763 kb on - strandat 239.872 kb on + strand, within PP_0188at 239.872 kb on + strand, within PP_0188at 239.873 kb on - strand, within PP_0188at 240.028 kb on + strand, within PP_0188at 240.161 kb on + strand, within PP_0188at 240.323 kb on + strand, within PP_0188at 240.324 kb on - strand, within PP_0188at 240.365 kb on - strand, within PP_0188at 240.402 kb on - strand, within PP_0188at 240.475 kb on + strand, within PP_0188at 240.483 kb on + strand, within PP_0188at 240.484 kb on - strand, within PP_0188at 240.575 kb on + strand, within PP_0188at 240.576 kb on - strand, within PP_0188at 240.652 kb on + strand, within PP_0188at 240.653 kb on - strand, within PP_0188at 240.755 kb on + strandat 240.756 kb on - strandat 240.783 kb on + strandat 240.783 kb on + strandat 240.783 kb on + strandat 240.784 kb on - strandat 240.784 kb on - strandat 240.785 kb on + strandat 240.786 kb on - strandat 240.786 kb on - strandat 240.786 kb on - strandat 240.842 kb on + strandat 240.842 kb on + strandat 240.843 kb on - strandat 240.911 kb on + strandat 240.911 kb on + strandat 240.911 kb on + strandat 240.912 kb on - strandat 240.912 kb on - strandat 240.912 kb on - strandat 240.915 kb on + strandat 240.916 kb on - strandat 240.926 kb on + strandat 240.927 kb on - strandat 241.082 kb on - strand, within PP_0189at 241.190 kb on + strand, within PP_0189at 241.191 kb on - strand, within PP_0189at 241.191 kb on - strand, within PP_0189at 241.191 kb on - strand, within PP_0189at 241.193 kb on + strand, within PP_0189at 241.193 kb on + strand, within PP_0189at 241.194 kb on - strand, within PP_0189at 241.194 kb on - strand, within PP_0189at 241.421 kb on + strand, within PP_0189at 241.421 kb on + strand, within PP_0189at 241.421 kb on + strand, within PP_0189at 241.422 kb on - strand, within PP_0189at 241.422 kb on - strand, within PP_0189at 241.563 kb on + strand, within PP_0189at 241.653 kb on - strand, within PP_0189at 241.674 kb on + strand, within PP_0189at 241.675 kb on - strand, within PP_0189at 241.728 kb on - strand, within PP_0189

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL
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239,685 + -0.2
239,685 + -0.5
239,685 + -0.1
239,685 + +0.1
239,685 + -0.9
239,686 - +0.7
239,693 + -1.8
239,694 - +0.3
239,694 - -0.0
239,730 + +0.1
239,730 + +1.3
239,731 - +1.3
239,731 - +0.3
239,731 - +2.6
239,762 + -1.0
239,763 - +0.6
239,763 - +0.2
239,763 - +0.2
239,872 + PP_0188 0.15 +0.8
239,872 + PP_0188 0.15 +1.0
239,873 - PP_0188 0.15 +1.2
240,028 + PP_0188 0.29 +0.0
240,161 + PP_0188 0.40 +1.0
240,323 + PP_0188 0.55 +0.1
240,324 - PP_0188 0.55 -0.0
240,365 - PP_0188 0.58 -0.6
240,402 - PP_0188 0.62 +0.7
240,475 + PP_0188 0.68 +1.3
240,483 + PP_0188 0.69 +1.2
240,484 - PP_0188 0.69 -0.3
240,575 + PP_0188 0.77 -0.2
240,576 - PP_0188 0.77 +0.6
240,652 + PP_0188 0.84 +1.0
240,653 - PP_0188 0.84 -0.6
240,755 + +0.2
240,756 - +1.4
240,783 + +0.0
240,783 + -0.1
240,783 + +0.8
240,784 - +0.0
240,784 - -0.5
240,785 + -0.4
240,786 - -0.5
240,786 - -0.3
240,786 - +0.2
240,842 + +0.5
240,842 + -0.5
240,843 - -2.0
240,911 + -0.2
240,911 + -0.3
240,911 + +1.6
240,912 - -2.7
240,912 - -1.1
240,912 - +1.5
240,915 + +1.7
240,916 - +0.5
240,926 + +0.3
240,927 - +0.4
241,082 - PP_0189 0.20 +1.5
241,190 + PP_0189 0.29 +1.5
241,191 - PP_0189 0.29 -0.1
241,191 - PP_0189 0.29 +0.1
241,191 - PP_0189 0.29 +1.3
241,193 + PP_0189 0.29 -1.3
241,193 + PP_0189 0.29 -1.0
241,194 - PP_0189 0.29 +0.6
241,194 - PP_0189 0.29 -0.6
241,421 + PP_0189 0.48 -0.3
241,421 + PP_0189 0.48 +1.0
241,421 + PP_0189 0.48 -0.2
241,422 - PP_0189 0.48 +0.6
241,422 - PP_0189 0.48 +0.3
241,563 + PP_0189 0.59 +1.0
241,653 - PP_0189 0.66 +1.4
241,674 + PP_0189 0.68 +0.3
241,675 - PP_0189 0.68 +0.5
241,728 - PP_0189 0.72 -0.6

Or see this region's nucleotide sequence