Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0186 and PP_0187 overlap by 4 nucleotides PP_0187 and PP_0188 are separated by 18 nucleotides
PP_0186: PP_0186 - Porphobilinogen deaminase, at 237,982 to 238,923
_0186
PP_0187: PP_0187 - Uroporphyrinogen-III synthase, at 238,920 to 239,687
_0187
PP_0188: PP_0188 - putative Uroporphyrin-III C-methyltransferase, at 239,706 to 240,833
_0188
Position (kb) 238
239
240 Strain fitness (log2 ratio) -2
-1
0
1
2 at 239.685 kb on + strand at 239.685 kb on + strand at 239.685 kb on + strand at 239.685 kb on + strand at 239.685 kb on + strand at 239.686 kb on - strand at 239.693 kb on + strand at 239.694 kb on - strand at 239.694 kb on - strand at 239.730 kb on + strand at 239.730 kb on + strand at 239.731 kb on - strand at 239.731 kb on - strand at 239.731 kb on - strand at 239.762 kb on + strand at 239.763 kb on - strand at 239.763 kb on - strand at 239.763 kb on - strand at 239.872 kb on + strand, within PP_0188 at 239.872 kb on + strand, within PP_0188 at 239.873 kb on - strand, within PP_0188 at 240.028 kb on + strand, within PP_0188 at 240.161 kb on + strand, within PP_0188 at 240.323 kb on + strand, within PP_0188 at 240.324 kb on - strand, within PP_0188 at 240.365 kb on - strand, within PP_0188 at 240.402 kb on - strand, within PP_0188 at 240.475 kb on + strand, within PP_0188 at 240.483 kb on + strand, within PP_0188 at 240.484 kb on - strand, within PP_0188 at 240.575 kb on + strand, within PP_0188 at 240.576 kb on - strand, within PP_0188 at 240.652 kb on + strand, within PP_0188 at 240.653 kb on - strand, within PP_0188
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 239,685 + -0.2 239,685 + -0.5 239,685 + -0.1 239,685 + +0.1 239,685 + -0.9 239,686 - +0.7 239,693 + -1.8 239,694 - +0.3 239,694 - -0.0 239,730 + +0.1 239,730 + +1.3 239,731 - +1.3 239,731 - +0.3 239,731 - +2.6 239,762 + -1.0 239,763 - +0.6 239,763 - +0.2 239,763 - +0.2 239,872 + PP_0188 0.15 +0.8 239,872 + PP_0188 0.15 +1.0 239,873 - PP_0188 0.15 +1.2 240,028 + PP_0188 0.29 +0.0 240,161 + PP_0188 0.40 +1.0 240,323 + PP_0188 0.55 +0.1 240,324 - PP_0188 0.55 -0.0 240,365 - PP_0188 0.58 -0.6 240,402 - PP_0188 0.62 +0.7 240,475 + PP_0188 0.68 +1.3 240,483 + PP_0188 0.69 +1.2 240,484 - PP_0188 0.69 -0.3 240,575 + PP_0188 0.77 -0.2 240,576 - PP_0188 0.77 +0.6 240,652 + PP_0188 0.84 +1.0 240,653 - PP_0188 0.84 -0.6
Or see this region's nucleotide sequence