Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0046 and PP_0047 are separated by 489 nucleotides PP_0047 and PP_0048 are separated by 117 nucleotides PP_0048 and PP_5423 are separated by 101 nucleotides
PP_0046: PP_0046 - tyrosine-specific outer membrane porin D, at 52,394 to 53,737
_0046
PP_0047: PP_0047 - response regulator, at 54,227 to 54,901
_0047
PP_0048: PP_0048 - conserved protein of unknown function, at 55,019 to 55,342
_0048
PP_5423: PP_5423 - conserved membrane protein of unknown function, at 55,444 to 55,971
_5423
Position (kb) 54
55 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 53.227 kb on + strand, within PP_0046 at 53.227 kb on + strand, within PP_0046 at 53.228 kb on - strand, within PP_0046 at 53.345 kb on + strand, within PP_0046 at 53.346 kb on - strand, within PP_0046 at 53.390 kb on + strand, within PP_0046 at 53.390 kb on + strand, within PP_0046 at 53.391 kb on - strand, within PP_0046 at 53.435 kb on + strand, within PP_0046 at 53.456 kb on + strand, within PP_0046 at 53.514 kb on - strand, within PP_0046 at 53.534 kb on + strand, within PP_0046 at 53.535 kb on - strand, within PP_0046 at 53.535 kb on - strand, within PP_0046 at 53.606 kb on + strand at 53.607 kb on - strand at 53.707 kb on - strand at 53.791 kb on + strand at 53.813 kb on + strand at 53.828 kb on + strand at 53.898 kb on - strand at 53.898 kb on - strand at 54.107 kb on - strand at 54.306 kb on + strand, within PP_0047 at 54.306 kb on + strand, within PP_0047 at 54.307 kb on - strand, within PP_0047 at 54.381 kb on + strand, within PP_0047 at 54.381 kb on + strand, within PP_0047 at 54.381 kb on + strand, within PP_0047 at 54.382 kb on - strand, within PP_0047 at 54.449 kb on - strand, within PP_0047 at 54.600 kb on + strand, within PP_0047 at 54.601 kb on - strand, within PP_0047 at 54.689 kb on + strand, within PP_0047 at 54.699 kb on + strand, within PP_0047 at 54.718 kb on + strand, within PP_0047 at 54.799 kb on - strand, within PP_0047 at 54.831 kb on + strand, within PP_0047 at 54.832 kb on - strand, within PP_0047 at 54.832 kb on - strand, within PP_0047 at 54.862 kb on + strand at 54.863 kb on - strand at 54.900 kb on - strand at 55.016 kb on + strand at 55.017 kb on - strand at 55.025 kb on + strand at 55.025 kb on + strand at 55.027 kb on + strand at 55.028 kb on - strand at 55.028 kb on - strand at 55.214 kb on + strand, within PP_0048 at 55.377 kb on - strand at 55.465 kb on - strand at 55.537 kb on + strand, within PP_5423 at 55.867 kb on - strand, within PP_5423 at 55.877 kb on - strand, within PP_5423
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 53,227 + PP_0046 0.62 +0.6 53,227 + PP_0046 0.62 -0.6 53,228 - PP_0046 0.62 -0.8 53,345 + PP_0046 0.71 +1.7 53,346 - PP_0046 0.71 -3.0 53,390 + PP_0046 0.74 +1.3 53,390 + PP_0046 0.74 +0.4 53,391 - PP_0046 0.74 -0.8 53,435 + PP_0046 0.77 +0.4 53,456 + PP_0046 0.79 +0.8 53,514 - PP_0046 0.83 +0.2 53,534 + PP_0046 0.85 +2.3 53,535 - PP_0046 0.85 -1.3 53,535 - PP_0046 0.85 +0.8 53,606 + -0.1 53,607 - -1.2 53,707 - -0.5 53,791 + -3.0 53,813 + +0.6 53,828 + +0.9 53,898 - +1.1 53,898 - +0.2 54,107 - +0.8 54,306 + PP_0047 0.12 +0.7 54,306 + PP_0047 0.12 -0.5 54,307 - PP_0047 0.12 -0.4 54,381 + PP_0047 0.23 +0.9 54,381 + PP_0047 0.23 +0.3 54,381 + PP_0047 0.23 +0.8 54,382 - PP_0047 0.23 +0.6 54,449 - PP_0047 0.33 +0.8 54,600 + PP_0047 0.55 +0.6 54,601 - PP_0047 0.55 +0.6 54,689 + PP_0047 0.68 +1.3 54,699 + PP_0047 0.70 +0.3 54,718 + PP_0047 0.73 -0.1 54,799 - PP_0047 0.85 -0.0 54,831 + PP_0047 0.89 +1.3 54,832 - PP_0047 0.90 +1.0 54,832 - PP_0047 0.90 -0.2 54,862 + +0.2 54,863 - -0.2 54,900 - +0.2 55,016 + -0.9 55,017 - +0.5 55,025 + +0.7 55,025 + -0.8 55,027 + -0.5 55,028 - -0.9 55,028 - +0.1 55,214 + PP_0048 0.60 -0.3 55,377 - +0.2 55,465 - +0.2 55,537 + PP_5423 0.18 -0.1 55,867 - PP_5423 0.80 +0.3 55,877 - PP_5423 0.82 +0.8
Or see this region's nucleotide sequence