Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5192 and PP_5193 are separated by 196 nucleotides PP_5193 and PP_5194 are separated by 51 nucleotides
PP_5192: PP_5192 - glycine dehydrogenase, at 5,921,557 to 5,924,430
_5192
PP_5193: PP_5193 - glycine cleavage system H protein 2, at 5,924,627 to 5,925,010
_5193
PP_5194: PP_5194 - aminomethyltransferase, at 5,925,062 to 5,926,144
_5194
Position (kb)
5924
5925
5926 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5923.815 kb on + strand, within PP_5192 at 5924.082 kb on - strand, within PP_5192 at 5924.090 kb on + strand, within PP_5192 at 5924.090 kb on + strand, within PP_5192 at 5924.090 kb on + strand, within PP_5192 at 5924.090 kb on + strand, within PP_5192 at 5924.099 kb on + strand, within PP_5192 at 5924.205 kb on - strand at 5924.447 kb on + strand at 5924.447 kb on + strand at 5924.470 kb on - strand at 5924.470 kb on - strand at 5924.477 kb on + strand at 5924.477 kb on + strand at 5924.478 kb on - strand at 5924.478 kb on - strand at 5924.548 kb on + strand at 5924.582 kb on + strand at 5924.582 kb on + strand at 5924.583 kb on - strand at 5924.590 kb on + strand at 5924.628 kb on + strand at 5924.629 kb on - strand at 5924.722 kb on - strand, within PP_5193 at 5924.722 kb on - strand, within PP_5193 at 5924.799 kb on + strand, within PP_5193 at 5924.799 kb on + strand, within PP_5193 at 5924.799 kb on + strand, within PP_5193 at 5924.799 kb on + strand, within PP_5193 at 5924.799 kb on + strand, within PP_5193 at 5924.799 kb on + strand, within PP_5193 at 5924.799 kb on + strand, within PP_5193 at 5924.799 kb on + strand, within PP_5193 at 5924.799 kb on + strand, within PP_5193 at 5924.799 kb on - strand, within PP_5193 at 5924.800 kb on - strand, within PP_5193 at 5924.800 kb on - strand, within PP_5193 at 5924.800 kb on - strand at 5924.800 kb on - strand, within PP_5193 at 5924.800 kb on - strand, within PP_5193 at 5924.800 kb on - strand, within PP_5193 at 5924.800 kb on - strand, within PP_5193 at 5924.800 kb on - strand, within PP_5193 at 5924.888 kb on + strand, within PP_5193 at 5925.053 kb on - strand at 5925.054 kb on + strand at 5925.055 kb on - strand at 5925.055 kb on - strand at 5925.164 kb on - strand at 5925.211 kb on + strand, within PP_5194 at 5925.212 kb on - strand, within PP_5194 at 5925.273 kb on - strand, within PP_5194 at 5925.780 kb on + strand, within PP_5194 at 5925.889 kb on + strand, within PP_5194 at 5925.889 kb on + strand, within PP_5194 at 5925.890 kb on - strand, within PP_5194 at 5925.891 kb on + strand, within PP_5194 at 5925.892 kb on - strand, within PP_5194 at 5925.892 kb on - strand, within PP_5194 at 5925.892 kb on - strand, within PP_5194 at 5925.892 kb on - strand, within PP_5194 at 5925.892 kb on - strand, within PP_5194 at 5925.892 kb on - strand, within PP_5194 at 5925.892 kb on - strand, within PP_5194 at 5925.992 kb on - strand, within PP_5194 at 5925.992 kb on - strand, within PP_5194
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 5,923,815 + PP_5192 0.79 -1.4 5,924,082 - PP_5192 0.88 -0.8 5,924,090 + PP_5192 0.88 -2.0 5,924,090 + PP_5192 0.88 -0.5 5,924,090 + PP_5192 0.88 -0.1 5,924,090 + PP_5192 0.88 +0.6 5,924,099 + PP_5192 0.88 -1.4 5,924,205 - +0.2 5,924,447 + +2.2 5,924,447 + +0.1 5,924,470 - -1.6 5,924,470 - -1.1 5,924,477 + +0.5 5,924,477 + -1.0 5,924,478 - -1.3 5,924,478 - +1.3 5,924,548 + -0.7 5,924,582 + -0.1 5,924,582 + +0.8 5,924,583 - -0.1 5,924,590 + +1.7 5,924,628 + -3.6 5,924,629 - -0.2 5,924,722 - PP_5193 0.25 +1.5 5,924,722 - PP_5193 0.25 -2.3 5,924,799 + PP_5193 0.45 +2.1 5,924,799 + PP_5193 0.45 +0.9 5,924,799 + PP_5193 0.45 -0.3 5,924,799 + PP_5193 0.45 +1.6 5,924,799 + PP_5193 0.45 +1.3 5,924,799 + PP_5193 0.45 -4.1 5,924,799 + PP_5193 0.45 +0.8 5,924,799 + PP_5193 0.45 +1.4 5,924,799 + PP_5193 0.45 +0.4 5,924,799 - PP_5193 0.45 +1.1 5,924,800 - PP_5193 0.45 +0.7 5,924,800 - PP_5193 0.45 -2.1 5,924,800 - +2.1 5,924,800 - PP_5193 0.45 -1.4 5,924,800 - PP_5193 0.45 +0.4 5,924,800 - PP_5193 0.45 +0.6 5,924,800 - PP_5193 0.45 -2.5 5,924,800 - PP_5193 0.45 -1.3 5,924,888 + PP_5193 0.68 +0.3 5,925,053 - -0.2 5,925,054 + +0.4 5,925,055 - -0.7 5,925,055 - -0.5 5,925,164 - +0.9 5,925,211 + PP_5194 0.14 -0.2 5,925,212 - PP_5194 0.14 +1.0 5,925,273 - PP_5194 0.19 -0.7 5,925,780 + PP_5194 0.66 -0.0 5,925,889 + PP_5194 0.76 +1.4 5,925,889 + PP_5194 0.76 -0.3 5,925,890 - PP_5194 0.76 +0.1 5,925,891 + PP_5194 0.77 -0.2 5,925,892 - PP_5194 0.77 +0.7 5,925,892 - PP_5194 0.77 -0.3 5,925,892 - PP_5194 0.77 -2.2 5,925,892 - PP_5194 0.77 +1.7 5,925,892 - PP_5194 0.77 +0.8 5,925,892 - PP_5194 0.77 +2.9 5,925,892 - PP_5194 0.77 -0.4 5,925,992 - PP_5194 0.86 +0.2 5,925,992 - PP_5194 0.86 -0.4
Or see this region's nucleotide sequence