Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4800 and PP_4801 are separated by 2 nucleotides PP_4801 and PP_4802 overlap by 4 nucleotides PP_4802 and PP_4803 are separated by 73 nucleotides
PP_4800: PP_4800 - Lipoyl synthase, at 5,463,448 to 5,464,464
_4800
PP_4801: PP_4801 - Octanoyltransferase, at 5,464,467 to 5,465,117
_4801
PP_4802: PP_4802 - putative lipoate regulatory protein, at 5,465,114 to 5,465,389
_4802
PP_4803: PP_4803 - D-alanyl-D-alanine carboxypeptidase, at 5,465,463 to 5,466,623
_4803
Position (kb) 5464
5465
5466 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5464.505 kb on - strand at 5465.407 kb on - strand at 5465.462 kb on - strand at 5465.462 kb on - strand at 5465.488 kb on - strand at 5465.682 kb on - strand, within PP_4803 at 5465.688 kb on - strand, within PP_4803 at 5465.688 kb on - strand, within PP_4803 at 5465.688 kb on - strand, within PP_4803 at 5465.688 kb on - strand, within PP_4803 at 5465.780 kb on - strand, within PP_4803 at 5466.034 kb on + strand at 5466.034 kb on + strand, within PP_4803 at 5466.035 kb on - strand, within PP_4803 at 5466.043 kb on + strand, within PP_4803 at 5466.043 kb on + strand, within PP_4803 at 5466.043 kb on + strand, within PP_4803 at 5466.107 kb on - strand, within PP_4803
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 5,464,505 - -1.1 5,465,407 - -1.1 5,465,462 - +1.5 5,465,462 - -0.5 5,465,488 - -0.7 5,465,682 - PP_4803 0.19 -2.6 5,465,688 - PP_4803 0.19 -1.1 5,465,688 - PP_4803 0.19 -2.9 5,465,688 - PP_4803 0.19 -3.6 5,465,688 - PP_4803 0.19 +0.2 5,465,780 - PP_4803 0.27 -2.8 5,466,034 + -0.7 5,466,034 + PP_4803 0.49 -0.7 5,466,035 - PP_4803 0.49 -3.4 5,466,043 + PP_4803 0.50 -2.8 5,466,043 + PP_4803 0.50 -0.5 5,466,043 + PP_4803 0.50 -2.1 5,466,107 - PP_4803 0.55 -1.8
Or see this region's nucleotide sequence