Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5644 and PP_3899 are separated by 167 nucleotides PP_3899 and PP_3900 are separated by 8 nucleotides PP_3900 and PP_3901 are separated by 149 nucleotides PP_3901 and PP_3902 are separated by 118 nucleotides PP_3902 and PP_3903 overlap by 11 nucleotides
PP_5644: PP_5644 - conserved membrane protein of unknown function, at 4,414,325 to 4,414,891
_5644
PP_3899: PP_3899 - conserved protein of unknown function, hicB family, at 4,415,059 to 4,415,391
_3899
PP_3900: PP_3900 - hypothetical protein, at 4,415,400 to 4,415,648
_3900
PP_3901: PP_3901 - conserved protein of unknown function, at 4,415,798 to 4,416,058
_3901
PP_3902: PP_3902 - conserved protein of unknown function, at 4,416,177 to 4,416,485
_3902
PP_3903: PP_3903 - conserved protein of unknown function, at 4,416,475 to 4,416,639
_3903
Position (kb) 4415
4416 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4414.432 kb on - strand, within PP_5644 at 4414.437 kb on + strand, within PP_5644 at 4414.489 kb on - strand, within PP_5644 at 4414.814 kb on + strand, within PP_5644 at 4414.863 kb on - strand at 4414.863 kb on - strand at 4414.866 kb on - strand at 4414.869 kb on + strand at 4414.870 kb on - strand at 4414.874 kb on + strand at 4415.018 kb on + strand at 4415.018 kb on + strand at 4415.019 kb on - strand at 4415.078 kb on + strand at 4415.079 kb on - strand at 4415.079 kb on - strand at 4415.150 kb on - strand, within PP_3899 at 4415.150 kb on - strand, within PP_3899 at 4415.430 kb on + strand, within PP_3900 at 4415.431 kb on - strand, within PP_3900 at 4415.431 kb on - strand, within PP_3900 at 4415.431 kb on - strand, within PP_3900 at 4415.433 kb on + strand, within PP_3900 at 4415.433 kb on + strand, within PP_3900 at 4415.434 kb on - strand, within PP_3900 at 4415.445 kb on - strand, within PP_3900 at 4415.450 kb on - strand, within PP_3900 at 4415.642 kb on + strand at 4415.643 kb on - strand at 4415.643 kb on - strand at 4415.736 kb on + strand at 4415.736 kb on + strand at 4415.737 kb on - strand at 4415.746 kb on + strand at 4415.746 kb on + strand at 4415.747 kb on - strand at 4415.775 kb on - strand at 4415.792 kb on - strand at 4415.792 kb on - strand at 4415.803 kb on + strand at 4415.924 kb on + strand, within PP_3901 at 4415.924 kb on + strand, within PP_3901 at 4415.924 kb on + strand, within PP_3901 at 4415.925 kb on - strand, within PP_3901 at 4415.925 kb on - strand, within PP_3901 at 4415.994 kb on + strand, within PP_3901 at 4415.995 kb on - strand, within PP_3901 at 4415.995 kb on - strand, within PP_3901 at 4415.995 kb on - strand, within PP_3901 at 4415.995 kb on - strand, within PP_3901 at 4416.049 kb on - strand at 4416.107 kb on + strand at 4416.126 kb on + strand at 4416.433 kb on + strand, within PP_3902 at 4416.433 kb on + strand, within PP_3902 at 4416.433 kb on + strand, within PP_3902 at 4416.434 kb on - strand, within PP_3902 at 4416.434 kb on - strand, within PP_3902 at 4416.518 kb on + strand, within PP_3903 at 4416.518 kb on + strand, within PP_3903 at 4416.519 kb on - strand, within PP_3903 at 4416.551 kb on + strand, within PP_3903 at 4416.637 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 4,414,432 - PP_5644 0.19 -2.9 4,414,437 + PP_5644 0.20 -0.4 4,414,489 - PP_5644 0.29 -0.4 4,414,814 + PP_5644 0.86 -1.5 4,414,863 - -0.1 4,414,863 - +1.8 4,414,866 - +0.1 4,414,869 + +1.2 4,414,870 - +1.6 4,414,874 + -1.0 4,415,018 + +1.5 4,415,018 + +1.1 4,415,019 - -0.1 4,415,078 + -0.2 4,415,079 - -0.7 4,415,079 - -0.1 4,415,150 - PP_3899 0.27 -0.2 4,415,150 - PP_3899 0.27 -0.9 4,415,430 + PP_3900 0.12 +1.5 4,415,431 - PP_3900 0.12 +0.4 4,415,431 - PP_3900 0.12 -0.5 4,415,431 - PP_3900 0.12 +0.1 4,415,433 + PP_3900 0.13 +2.0 4,415,433 + PP_3900 0.13 +1.6 4,415,434 - PP_3900 0.14 +0.3 4,415,445 - PP_3900 0.18 +0.2 4,415,450 - PP_3900 0.20 +0.2 4,415,642 + +1.2 4,415,643 - +0.9 4,415,643 - -1.4 4,415,736 + -2.8 4,415,736 + +1.2 4,415,737 - -0.0 4,415,746 + -0.8 4,415,746 + +0.2 4,415,747 - +0.2 4,415,775 - +0.2 4,415,792 - +0.6 4,415,792 - -0.2 4,415,803 + +0.5 4,415,924 + PP_3901 0.48 +0.9 4,415,924 + PP_3901 0.48 +0.4 4,415,924 + PP_3901 0.48 -1.3 4,415,925 - PP_3901 0.49 -1.8 4,415,925 - PP_3901 0.49 -0.1 4,415,994 + PP_3901 0.75 -0.3 4,415,995 - PP_3901 0.75 -0.3 4,415,995 - PP_3901 0.75 +0.1 4,415,995 - PP_3901 0.75 +0.2 4,415,995 - PP_3901 0.75 -0.1 4,416,049 - +1.1 4,416,107 + -0.5 4,416,126 + -2.9 4,416,433 + PP_3902 0.83 -0.9 4,416,433 + PP_3902 0.83 -1.6 4,416,433 + PP_3902 0.83 -0.8 4,416,434 - PP_3902 0.83 +1.1 4,416,434 - PP_3902 0.83 +1.3 4,416,518 + PP_3903 0.26 -2.4 4,416,518 + PP_3903 0.26 -1.9 4,416,519 - PP_3903 0.27 +0.1 4,416,551 + PP_3903 0.46 -2.0 4,416,637 + +1.0
Or see this region's nucleotide sequence