Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2893 and PP_2894 are separated by 19 nucleotides PP_2894 and PP_2895 are separated by 75 nucleotides PP_2895 and PP_2896 are separated by 65 nucleotides PP_2896 and PP_2898 are separated by 251 nucleotides
PP_2893: PP_2893 - conserved exported protein of unknown function, at 3,290,717 to 3,294,391
_2893
PP_2894: PP_2894 - conserved protein of unknown function, at 3,294,411 to 3,294,965
_2894
PP_2895: PP_2895 - conserved protein of unknown function, at 3,295,041 to 3,295,685
_2895
PP_2896: PP_2896 - conserved protein of unknown function, at 3,295,751 to 3,296,131
_2896
PP_2898: PP_2898 - conserved exported protein of unknown function, at 3,296,383 to 3,296,874
_2898
Position (kb)
3295
3296 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3294.048 kb on - strand at 3294.077 kb on - strand at 3294.088 kb on + strand at 3294.088 kb on + strand at 3294.089 kb on - strand at 3294.089 kb on - strand at 3294.235 kb on + strand at 3294.309 kb on - strand at 3294.309 kb on - strand at 3294.309 kb on - strand at 3294.329 kb on + strand at 3294.330 kb on - strand at 3294.375 kb on - strand at 3294.390 kb on - strand at 3294.499 kb on + strand, within PP_2894 at 3294.499 kb on + strand, within PP_2894 at 3294.499 kb on + strand, within PP_2894 at 3294.500 kb on - strand, within PP_2894 at 3294.500 kb on - strand, within PP_2894 at 3294.729 kb on - strand, within PP_2894 at 3294.862 kb on + strand, within PP_2894 at 3294.862 kb on + strand, within PP_2894 at 3295.042 kb on + strand at 3295.095 kb on + strand at 3295.238 kb on + strand, within PP_2895 at 3295.239 kb on - strand, within PP_2895 at 3295.374 kb on - strand, within PP_2895 at 3295.374 kb on - strand, within PP_2895 at 3295.377 kb on + strand, within PP_2895 at 3295.389 kb on + strand, within PP_2895 at 3295.389 kb on + strand, within PP_2895 at 3295.390 kb on - strand, within PP_2895 at 3295.548 kb on + strand, within PP_2895 at 3295.548 kb on + strand, within PP_2895 at 3295.549 kb on - strand, within PP_2895 at 3295.662 kb on + strand at 3295.663 kb on - strand at 3295.664 kb on + strand at 3295.664 kb on + strand at 3295.673 kb on + strand at 3295.673 kb on + strand at 3295.674 kb on - strand at 3295.703 kb on - strand at 3295.703 kb on - strand at 3295.706 kb on + strand at 3295.706 kb on + strand at 3295.707 kb on - strand at 3295.714 kb on + strand at 3295.714 kb on + strand at 3295.729 kb on + strand at 3295.730 kb on - strand at 3295.810 kb on - strand, within PP_2896 at 3295.810 kb on - strand, within PP_2896 at 3296.005 kb on - strand, within PP_2896 at 3296.005 kb on - strand, within PP_2896 at 3296.005 kb on - strand, within PP_2896 at 3296.005 kb on - strand, within PP_2896 at 3296.005 kb on - strand, within PP_2896 at 3296.040 kb on + strand, within PP_2896 at 3296.060 kb on - strand, within PP_2896 at 3296.240 kb on + strand at 3296.256 kb on + strand at 3296.257 kb on - strand at 3296.257 kb on - strand at 3296.257 kb on - strand at 3296.257 kb on - strand at 3296.301 kb on - strand at 3296.372 kb on + strand at 3296.426 kb on + strand at 3296.551 kb on + strand, within PP_2898 at 3296.551 kb on + strand, within PP_2898 at 3296.552 kb on - strand, within PP_2898
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 3,294,048 - +0.2 3,294,077 - +1.1 3,294,088 + +0.5 3,294,088 + +2.1 3,294,089 - +0.4 3,294,089 - +0.7 3,294,235 + +1.6 3,294,309 - +0.8 3,294,309 - +1.2 3,294,309 - +0.6 3,294,329 + +0.5 3,294,330 - +1.1 3,294,375 - +1.0 3,294,390 - -0.9 3,294,499 + PP_2894 0.16 -2.1 3,294,499 + PP_2894 0.16 +0.8 3,294,499 + PP_2894 0.16 +0.3 3,294,500 - PP_2894 0.16 +0.9 3,294,500 - PP_2894 0.16 +0.8 3,294,729 - PP_2894 0.57 +0.6 3,294,862 + PP_2894 0.81 -1.6 3,294,862 + PP_2894 0.81 +0.3 3,295,042 + +1.9 3,295,095 + +0.4 3,295,238 + PP_2895 0.31 +1.4 3,295,239 - PP_2895 0.31 -0.9 3,295,374 - PP_2895 0.52 +0.4 3,295,374 - PP_2895 0.52 +1.0 3,295,377 + PP_2895 0.52 +1.0 3,295,389 + PP_2895 0.54 -0.6 3,295,389 + PP_2895 0.54 +0.7 3,295,390 - PP_2895 0.54 +1.3 3,295,548 + PP_2895 0.79 +1.2 3,295,548 + PP_2895 0.79 -1.0 3,295,549 - PP_2895 0.79 +0.5 3,295,662 + +1.2 3,295,663 - -0.8 3,295,664 + -1.4 3,295,664 + +0.5 3,295,673 + -0.7 3,295,673 + +0.3 3,295,674 - -1.3 3,295,703 - -2.5 3,295,703 - +1.1 3,295,706 + -1.0 3,295,706 + -0.2 3,295,707 - -1.2 3,295,714 + -3.0 3,295,714 + +0.3 3,295,729 + -0.4 3,295,730 - +0.6 3,295,810 - PP_2896 0.15 +1.7 3,295,810 - PP_2896 0.15 -0.5 3,296,005 - PP_2896 0.67 -0.7 3,296,005 - PP_2896 0.67 -0.2 3,296,005 - PP_2896 0.67 -0.4 3,296,005 - PP_2896 0.67 -0.4 3,296,005 - PP_2896 0.67 -0.2 3,296,040 + PP_2896 0.76 +0.0 3,296,060 - PP_2896 0.81 -0.6 3,296,240 + -0.8 3,296,256 + -0.8 3,296,257 - -0.1 3,296,257 - -0.5 3,296,257 - -3.7 3,296,257 - -0.4 3,296,301 - +0.4 3,296,372 + -1.7 3,296,426 + -1.3 3,296,551 + PP_2898 0.34 -0.2 3,296,551 + PP_2898 0.34 -0.7 3,296,552 - PP_2898 0.34 -1.1
Or see this region's nucleotide sequence