Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4727 and PP_4728 are separated by 101 nucleotides PP_4728 and PP_4729 are separated by 192 nucleotides PP_4729 and PP_4730 are separated by 107 nucleotides PP_4730 and PP_4731 are separated by 97 nucleotides
PP_4727: PP_4727 - Chaperone protein DnaK, at 5,375,639 to 5,377,564
_4727
PP_4728: PP_4728 - Protein GrpE, at 5,377,666 to 5,378,223
_4728
PP_4729: PP_4729 - factor used in recombination and DNA repair, at 5,378,416 to 5,380,089
_4729
PP_4730: PP_4730 - ferric iron uptake global transcriptional repressor Fur-Fe+2, at 5,380,197 to 5,380,601
_4730
PP_4731: PP_4731 - Outer membrane protein assembly factor BamE, at 5,380,699 to 5,381,235
_4731
Position (kb)
5378
5379
5380
5381 Strain fitness (log2 ratio)
-2
-1
0
1 at 5377.624 kb on - strand at 5377.643 kb on - strand at 5378.282 kb on - strand at 5378.282 kb on - strand at 5378.284 kb on - strand at 5378.284 kb on - strand at 5378.284 kb on - strand at 5378.318 kb on + strand at 5378.318 kb on + strand at 5378.319 kb on - strand at 5378.319 kb on - strand at 5378.319 kb on - strand at 5378.338 kb on + strand at 5378.339 kb on - strand at 5378.339 kb on - strand at 5378.339 kb on - strand at 5378.339 kb on - strand at 5378.443 kb on + strand at 5378.443 kb on + strand at 5378.443 kb on + strand at 5378.444 kb on - strand at 5378.860 kb on + strand, within PP_4729 at 5378.861 kb on - strand, within PP_4729 at 5378.892 kb on - strand, within PP_4729 at 5378.990 kb on - strand, within PP_4729 at 5378.990 kb on - strand, within PP_4729 at 5379.340 kb on + strand, within PP_4729 at 5379.340 kb on + strand, within PP_4729 at 5379.340 kb on + strand, within PP_4729 at 5379.341 kb on - strand, within PP_4729 at 5379.341 kb on - strand, within PP_4729 at 5379.341 kb on - strand, within PP_4729 at 5379.341 kb on - strand, within PP_4729 at 5379.424 kb on - strand, within PP_4729 at 5379.484 kb on + strand, within PP_4729 at 5379.484 kb on + strand, within PP_4729 at 5379.484 kb on + strand, within PP_4729 at 5379.485 kb on - strand, within PP_4729 at 5379.487 kb on - strand, within PP_4729 at 5379.487 kb on - strand, within PP_4729 at 5379.487 kb on - strand, within PP_4729 at 5379.732 kb on + strand, within PP_4729 at 5379.733 kb on - strand, within PP_4729 at 5379.875 kb on + strand, within PP_4729 at 5379.875 kb on + strand, within PP_4729 at 5379.876 kb on - strand, within PP_4729 at 5379.876 kb on - strand, within PP_4729 at 5379.975 kb on + strand at 5380.198 kb on + strand at 5380.209 kb on - strand at 5380.209 kb on - strand at 5380.209 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 5,377,624 - +0.2 5,377,643 - -2.6 5,378,282 - +0.1 5,378,282 - +1.2 5,378,284 - -0.9 5,378,284 - -1.0 5,378,284 - +0.7 5,378,318 + -0.4 5,378,318 + -0.1 5,378,319 - -2.2 5,378,319 - +0.2 5,378,319 - +0.1 5,378,338 + +0.9 5,378,339 - +1.0 5,378,339 - -0.4 5,378,339 - -0.3 5,378,339 - +0.2 5,378,443 + -0.0 5,378,443 + +1.7 5,378,443 + -2.6 5,378,444 - -1.0 5,378,860 + PP_4729 0.27 -0.4 5,378,861 - PP_4729 0.27 +0.0 5,378,892 - PP_4729 0.28 -0.1 5,378,990 - PP_4729 0.34 -1.4 5,378,990 - PP_4729 0.34 -1.0 5,379,340 + PP_4729 0.55 +0.3 5,379,340 + PP_4729 0.55 +0.1 5,379,340 + PP_4729 0.55 -1.5 5,379,341 - PP_4729 0.55 +0.3 5,379,341 - PP_4729 0.55 -1.2 5,379,341 - PP_4729 0.55 +0.4 5,379,341 - PP_4729 0.55 -0.8 5,379,424 - PP_4729 0.60 +0.1 5,379,484 + PP_4729 0.64 -0.4 5,379,484 + PP_4729 0.64 -1.0 5,379,484 + PP_4729 0.64 -0.3 5,379,485 - PP_4729 0.64 -2.4 5,379,487 - PP_4729 0.64 -0.6 5,379,487 - PP_4729 0.64 -0.3 5,379,487 - PP_4729 0.64 -0.3 5,379,732 + PP_4729 0.79 -0.1 5,379,733 - PP_4729 0.79 +0.4 5,379,875 + PP_4729 0.87 -0.8 5,379,875 + PP_4729 0.87 -1.1 5,379,876 - PP_4729 0.87 +0.4 5,379,876 - PP_4729 0.87 -1.0 5,379,975 + -0.6 5,380,198 + -0.2 5,380,209 - -0.9 5,380,209 - -0.5 5,380,209 - -1.1
Or see this region's nucleotide sequence