Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2297 and PP_t40 are separated by 299 nucleotides PP_t40 and PP_2298 are separated by 149 nucleotides PP_2298 and PP_2299 are separated by 230 nucleotides
PP_2297: PP_2297 - putative integrative genetic element Ppu40, integrase, at 2,624,740 to 2,625,822
_2297
PP_t40: PP_t40 - tRNA-His, at 2,626,122 to 2,626,197
_t40
PP_2298: PP_2298 - conserved protein of unknown function, at 2,626,347 to 2,626,685
_2298
PP_2299: PP_2299 - Trigger factor, at 2,626,916 to 2,628,247
_2299
Position (kb)
2626
2627 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2625.400 kb on + strand, within PP_2297 at 2625.444 kb on + strand, within PP_2297 at 2625.550 kb on + strand, within PP_2297 at 2625.550 kb on + strand, within PP_2297 at 2625.551 kb on - strand, within PP_2297 at 2625.737 kb on + strand at 2625.799 kb on + strand at 2625.799 kb on + strand at 2625.799 kb on + strand at 2625.799 kb on + strand at 2625.799 kb on + strand at 2625.799 kb on + strand at 2625.800 kb on - strand at 2625.800 kb on - strand at 2625.800 kb on - strand at 2625.800 kb on - strand at 2625.810 kb on + strand at 2625.811 kb on - strand at 2625.811 kb on - strand at 2625.860 kb on - strand at 2625.860 kb on - strand at 2625.860 kb on - strand at 2625.951 kb on + strand at 2625.951 kb on + strand at 2625.951 kb on + strand at 2625.951 kb on + strand at 2625.951 kb on + strand at 2625.952 kb on - strand at 2625.952 kb on - strand at 2625.952 kb on - strand at 2625.991 kb on + strand at 2625.992 kb on - strand at 2626.057 kb on + strand at 2626.094 kb on + strand at 2626.094 kb on + strand at 2626.095 kb on - strand at 2626.102 kb on + strand at 2626.119 kb on - strand at 2626.142 kb on - strand, within PP_t40 at 2626.198 kb on + strand at 2626.302 kb on - strand at 2626.302 kb on - strand at 2626.302 kb on - strand at 2626.536 kb on - strand, within PP_2298 at 2626.653 kb on + strand at 2626.707 kb on + strand at 2626.724 kb on - strand at 2626.866 kb on + strand at 2626.867 kb on - strand at 2626.930 kb on - strand at 2627.075 kb on - strand, within PP_2299 at 2627.080 kb on - strand, within PP_2299 at 2627.208 kb on + strand, within PP_2299 at 2627.209 kb on - strand, within PP_2299 at 2627.541 kb on + strand, within PP_2299 at 2627.541 kb on + strand, within PP_2299 at 2627.664 kb on + strand, within PP_2299 at 2627.664 kb on + strand, within PP_2299 at 2627.665 kb on - strand, within PP_2299
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 2,625,400 + PP_2297 0.61 -2.1 2,625,444 + PP_2297 0.65 -1.6 2,625,550 + PP_2297 0.75 +0.4 2,625,550 + PP_2297 0.75 -2.9 2,625,551 - PP_2297 0.75 -0.4 2,625,737 + -0.1 2,625,799 + -0.7 2,625,799 + -0.6 2,625,799 + -0.6 2,625,799 + -0.5 2,625,799 + +0.7 2,625,799 + -1.3 2,625,800 - +0.0 2,625,800 - +0.0 2,625,800 - -1.9 2,625,800 - -1.2 2,625,810 + +0.0 2,625,811 - +0.5 2,625,811 - -1.6 2,625,860 - +0.2 2,625,860 - -0.6 2,625,860 - -0.1 2,625,951 + -0.2 2,625,951 + +1.0 2,625,951 + -1.6 2,625,951 + -0.7 2,625,951 + +0.2 2,625,952 - -1.1 2,625,952 - -2.0 2,625,952 - -1.6 2,625,991 + -0.3 2,625,992 - -0.7 2,626,057 + -0.9 2,626,094 + -1.2 2,626,094 + -0.9 2,626,095 - -1.7 2,626,102 + -0.3 2,626,119 - -2.0 2,626,142 - PP_t40 0.26 -0.1 2,626,198 + -0.3 2,626,302 - -1.2 2,626,302 - +0.5 2,626,302 - -0.3 2,626,536 - PP_2298 0.56 -1.8 2,626,653 + +0.1 2,626,707 + -1.9 2,626,724 - -2.6 2,626,866 + +0.3 2,626,867 - -3.2 2,626,930 - -4.5 2,627,075 - PP_2299 0.12 -4.5 2,627,080 - PP_2299 0.12 -1.9 2,627,208 + PP_2299 0.22 -4.1 2,627,209 - PP_2299 0.22 -2.6 2,627,541 + PP_2299 0.47 -2.5 2,627,541 + PP_2299 0.47 -2.7 2,627,664 + PP_2299 0.56 +0.3 2,627,664 + PP_2299 0.56 -2.2 2,627,665 - PP_2299 0.56 -2.4
Or see this region's nucleotide sequence