Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0185 and PP_0186 are separated by 118 nucleotides PP_0186 and PP_0187 overlap by 4 nucleotides PP_0187 and PP_0188 are separated by 18 nucleotides
PP_0185: PP_0185 - Positive alginate biosynthesis regulatory protein, at 237,120 to 237,863
_0185
PP_0186: PP_0186 - Porphobilinogen deaminase, at 237,982 to 238,923
_0186
PP_0187: PP_0187 - Uroporphyrinogen-III synthase, at 238,920 to 239,687
_0187
PP_0188: PP_0188 - putative Uroporphyrin-III C-methyltransferase, at 239,706 to 240,833
_0188
Position (kb) 237
238
239 Strain fitness (log2 ratio) -2
-1
0
1
2 at 237.076 kb on + strand at 237.076 kb on + strand at 237.076 kb on + strand at 237.077 kb on - strand at 237.081 kb on + strand at 237.081 kb on + strand at 237.081 kb on + strand at 237.082 kb on - strand at 237.082 kb on - strand at 237.082 kb on - strand at 237.083 kb on + strand at 237.083 kb on + strand at 237.083 kb on + strand at 237.083 kb on + strand at 237.084 kb on - strand at 237.084 kb on - strand at 237.084 kb on - strand at 237.084 kb on - strand at 237.084 kb on - strand at 237.119 kb on + strand at 237.119 kb on + strand at 237.120 kb on - strand at 237.195 kb on + strand, within PP_0185 at 237.195 kb on + strand, within PP_0185 at 237.195 kb on + strand, within PP_0185 at 237.196 kb on - strand, within PP_0185 at 237.379 kb on - strand, within PP_0185 at 237.379 kb on - strand, within PP_0185 at 237.379 kb on - strand, within PP_0185 at 237.540 kb on - strand, within PP_0185 at 237.594 kb on + strand, within PP_0185 at 237.615 kb on + strand, within PP_0185 at 237.615 kb on + strand, within PP_0185 at 237.615 kb on + strand, within PP_0185 at 237.615 kb on + strand, within PP_0185 at 237.615 kb on + strand, within PP_0185 at 237.615 kb on + strand at 237.615 kb on + strand, within PP_0185 at 237.615 kb on + strand, within PP_0185 at 237.615 kb on + strand, within PP_0185 at 237.616 kb on - strand, within PP_0185 at 237.616 kb on - strand, within PP_0185 at 237.616 kb on - strand, within PP_0185 at 237.616 kb on - strand, within PP_0185 at 237.616 kb on - strand, within PP_0185 at 237.781 kb on - strand, within PP_0185 at 237.902 kb on - strand at 239.685 kb on + strand at 239.685 kb on + strand at 239.685 kb on + strand at 239.685 kb on + strand at 239.685 kb on + strand at 239.686 kb on - strand at 239.693 kb on + strand at 239.694 kb on - strand at 239.694 kb on - strand at 239.730 kb on + strand at 239.730 kb on + strand at 239.731 kb on - strand at 239.731 kb on - strand at 239.731 kb on - strand at 239.762 kb on + strand at 239.763 kb on - strand at 239.763 kb on - strand at 239.763 kb on - strand at 239.872 kb on + strand, within PP_0188 at 239.872 kb on + strand, within PP_0188 at 239.873 kb on - strand, within PP_0188
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 237,076 + +0.1 237,076 + +0.2 237,076 + -0.2 237,077 - +1.2 237,081 + +0.3 237,081 + +0.8 237,081 + +0.4 237,082 - +0.5 237,082 - +0.6 237,082 - +1.8 237,083 + -0.2 237,083 + -0.1 237,083 + +0.2 237,083 + -1.0 237,084 - +1.1 237,084 - +1.0 237,084 - +1.1 237,084 - +0.6 237,084 - +1.5 237,119 + +0.4 237,119 + +1.6 237,120 - +1.3 237,195 + PP_0185 0.10 +1.0 237,195 + PP_0185 0.10 +0.3 237,195 + PP_0185 0.10 +0.8 237,196 - PP_0185 0.10 +1.1 237,379 - PP_0185 0.35 +1.1 237,379 - PP_0185 0.35 +0.8 237,379 - PP_0185 0.35 +1.3 237,540 - PP_0185 0.56 +0.1 237,594 + PP_0185 0.64 +0.9 237,615 + PP_0185 0.67 +0.1 237,615 + PP_0185 0.67 +1.2 237,615 + PP_0185 0.67 +0.6 237,615 + PP_0185 0.67 +0.5 237,615 + PP_0185 0.67 +1.0 237,615 + +0.2 237,615 + PP_0185 0.67 +0.6 237,615 + PP_0185 0.67 +0.7 237,615 + PP_0185 0.67 +0.2 237,616 - PP_0185 0.67 +1.1 237,616 - PP_0185 0.67 +0.2 237,616 - PP_0185 0.67 +0.3 237,616 - PP_0185 0.67 -0.1 237,616 - PP_0185 0.67 +1.1 237,781 - PP_0185 0.89 +0.9 237,902 - -2.4 239,685 + -0.2 239,685 + -0.8 239,685 + -0.5 239,685 + +0.8 239,685 + -0.2 239,686 - -0.9 239,693 + -2.0 239,694 - -0.9 239,694 - +0.4 239,730 + +0.8 239,730 + +1.0 239,731 - +1.0 239,731 - -0.1 239,731 - +2.1 239,762 + +1.2 239,763 - +1.6 239,763 - +0.8 239,763 - +1.1 239,872 + PP_0188 0.15 -0.5 239,872 + PP_0188 0.15 +0.6 239,873 - PP_0188 0.15 +0.9
Or see this region's nucleotide sequence