Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2095 and PP_5502 are separated by 143 nucleotides PP_5502 and PP_2096 are separated by 517 nucleotides
PP_2095: PP_2095 - Dihydroorotate dehydrogenase (quinone), at 2,387,241 to 2,388,263
_2095
PP_5502: PP_5502 - ribosome modulation factor, at 2,388,407 to 2,388,622
_5502
PP_2096: PP_2096 - fused 23S rRNA m2G2445 methyltransferase and 23S rRNA m7G2069 methyltransferase, at 2,389,140 to 2,391,377
_2096
Position (kb)
2388
2389 Strain fitness (log2 ratio)
-1
0
1
2 at 2388.320 kb on + strand at 2388.384 kb on + strand at 2388.384 kb on + strand at 2388.437 kb on - strand, within PP_5502 at 2388.472 kb on + strand, within PP_5502 at 2388.573 kb on + strand, within PP_5502 at 2388.585 kb on + strand, within PP_5502 at 2388.585 kb on + strand, within PP_5502 at 2388.586 kb on - strand, within PP_5502 at 2388.586 kb on - strand, within PP_5502 at 2388.623 kb on - strand at 2388.623 kb on - strand at 2388.649 kb on + strand at 2388.649 kb on + strand at 2388.649 kb on + strand at 2388.649 kb on + strand at 2388.649 kb on + strand at 2388.650 kb on - strand at 2388.650 kb on - strand at 2388.650 kb on - strand at 2388.650 kb on - strand at 2388.650 kb on - strand at 2388.650 kb on - strand at 2388.765 kb on - strand at 2388.803 kb on + strand at 2388.803 kb on + strand at 2388.803 kb on + strand at 2388.804 kb on - strand at 2388.811 kb on + strand at 2388.835 kb on - strand at 2388.835 kb on - strand at 2388.991 kb on - strand at 2388.996 kb on + strand at 2388.996 kb on + strand at 2389.011 kb on + strand at 2389.011 kb on + strand at 2389.011 kb on + strand at 2389.011 kb on + strand at 2389.011 kb on + strand at 2389.011 kb on + strand at 2389.011 kb on + strand at 2389.012 kb on - strand at 2389.012 kb on - strand at 2389.012 kb on - strand at 2389.012 kb on - strand at 2389.012 kb on - strand at 2389.012 kb on - strand at 2389.012 kb on - strand at 2389.012 kb on - strand at 2389.118 kb on + strand at 2389.162 kb on + strand at 2389.180 kb on + strand at 2389.180 kb on + strand at 2389.181 kb on - strand at 2389.207 kb on - strand at 2389.364 kb on - strand, within PP_2096 at 2389.410 kb on + strand, within PP_2096 at 2389.410 kb on + strand, within PP_2096 at 2389.411 kb on - strand, within PP_2096
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 2,388,320 + -0.4 2,388,384 + +0.5 2,388,384 + +1.4 2,388,437 - PP_5502 0.14 +1.3 2,388,472 + PP_5502 0.30 +0.6 2,388,573 + PP_5502 0.77 +0.6 2,388,585 + PP_5502 0.82 +1.0 2,388,585 + PP_5502 0.82 +1.1 2,388,586 - PP_5502 0.83 +1.0 2,388,586 - PP_5502 0.83 +1.7 2,388,623 - +1.6 2,388,623 - +0.2 2,388,649 + +0.8 2,388,649 + +1.3 2,388,649 + +1.6 2,388,649 + +1.4 2,388,649 + +0.4 2,388,650 - +1.2 2,388,650 - +0.6 2,388,650 - +1.4 2,388,650 - -0.1 2,388,650 - -0.5 2,388,650 - +0.4 2,388,765 - +1.1 2,388,803 + +1.2 2,388,803 + +1.3 2,388,803 + +1.8 2,388,804 - +0.8 2,388,811 + +1.0 2,388,835 - +0.1 2,388,835 - +0.8 2,388,991 - -0.5 2,388,996 + +0.8 2,388,996 + +0.9 2,389,011 + +1.0 2,389,011 + +2.7 2,389,011 + +1.4 2,389,011 + +0.5 2,389,011 + +1.3 2,389,011 + +1.0 2,389,011 + +0.7 2,389,012 - -0.1 2,389,012 - +0.5 2,389,012 - -1.6 2,389,012 - +0.4 2,389,012 - +0.2 2,389,012 - -0.3 2,389,012 - -0.1 2,389,012 - +0.1 2,389,118 + -0.3 2,389,162 + +1.0 2,389,180 + +0.4 2,389,180 + -0.2 2,389,181 - +1.2 2,389,207 - +0.1 2,389,364 - PP_2096 0.10 -0.6 2,389,410 + PP_2096 0.12 +0.4 2,389,410 + PP_2096 0.12 -0.1 2,389,411 - PP_2096 0.12 +2.6
Or see this region's nucleotide sequence