Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3540 and PP_3541 are separated by 532 nucleotides PP_3541 and PP_3542 are separated by 168 nucleotides PP_3542 and PP_3543 are separated by 121 nucleotides
PP_3540: PP_3540 - Hydroxymethylglutaryl-CoA lyase, at 4,012,165 to 4,013,064
_3540
PP_3541: PP_3541 - Transporter, MgtC family, at 4,013,597 to 4,014,115
_3541
PP_3542: PP_3542 - conserved protein of unknown function, at 4,014,284 to 4,014,523
_3542
PP_3543: PP_3543 - Iron-sulfur cluster-binding protein, at 4,014,645 to 4,016,048
_3543
Position (kb)
4013
4014
4015 Strain fitness (log2 ratio)
-2
-1
0
1 at 4013.062 kb on + strand at 4013.062 kb on + strand at 4013.062 kb on + strand at 4013.062 kb on + strand at 4013.063 kb on - strand at 4013.100 kb on + strand at 4013.100 kb on + strand at 4013.101 kb on - strand at 4013.235 kb on + strand at 4013.236 kb on - strand at 4013.236 kb on - strand at 4013.237 kb on + strand at 4013.401 kb on + strand at 4013.401 kb on + strand at 4013.402 kb on - strand at 4013.412 kb on - strand at 4013.467 kb on + strand at 4013.533 kb on + strand at 4013.534 kb on - strand at 4013.534 kb on - strand at 4013.550 kb on + strand at 4013.551 kb on - strand at 4013.682 kb on - strand, within PP_3541 at 4013.765 kb on + strand, within PP_3541 at 4013.815 kb on + strand, within PP_3541 at 4014.142 kb on + strand at 4014.199 kb on - strand at 4014.199 kb on - strand at 4014.261 kb on - strand at 4014.519 kb on + strand at 4014.519 kb on + strand at 4014.562 kb on + strand at 4014.605 kb on + strand at 4014.606 kb on - strand at 4014.606 kb on - strand at 4014.654 kb on - strand at 4014.654 kb on - strand at 4014.666 kb on - strand at 4014.725 kb on + strand at 4014.748 kb on - strand at 4014.752 kb on + strand at 4014.752 kb on + strand at 4014.958 kb on - strand, within PP_3543 at 4014.981 kb on + strand, within PP_3543
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 4,013,062 + -2.0 4,013,062 + +0.5 4,013,062 + -1.0 4,013,062 + +0.1 4,013,063 - +0.3 4,013,100 + -0.3 4,013,100 + -1.0 4,013,101 - -0.0 4,013,235 + -0.1 4,013,236 - -0.5 4,013,236 - +0.1 4,013,237 + -0.7 4,013,401 + -1.6 4,013,401 + -0.7 4,013,402 - -1.1 4,013,412 - -1.1 4,013,467 + -1.7 4,013,533 + -0.3 4,013,534 - +0.1 4,013,534 - -0.0 4,013,550 + +0.4 4,013,551 - +0.7 4,013,682 - PP_3541 0.16 +0.6 4,013,765 + PP_3541 0.32 +0.6 4,013,815 + PP_3541 0.42 +0.6 4,014,142 + +0.4 4,014,199 - +0.8 4,014,199 - +0.4 4,014,261 - +0.6 4,014,519 + -0.7 4,014,519 + +1.1 4,014,562 + +0.3 4,014,605 + -1.0 4,014,606 - +0.2 4,014,606 - -1.6 4,014,654 - +0.3 4,014,654 - -0.5 4,014,666 - +1.5 4,014,725 + +0.3 4,014,748 - -0.2 4,014,752 + -0.6 4,014,752 + -0.2 4,014,958 - PP_3543 0.22 -0.3 4,014,981 + PP_3543 0.24 +0.7
Or see this region's nucleotide sequence