Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2746 and PP_2747 are separated by 20 nucleotides PP_2747 and PP_2748 overlap by 34 nucleotides PP_2748 and PP_2749 overlap by 4 nucleotides
PP_2746: PP_2746 - dimethyl-sulfide monooxygenase, at 3,128,754 to 3,130,163
_2746
PP_2747: PP_2747 - conserved protein of unknown function, at 3,130,184 to 3,131,155
_2747
PP_2748: PP_2748 - putative Branched-chain amino acid ABC transporter, ATP-binding protein, at 3,131,122 to 3,131,922
_2748
PP_2749: PP_2749 - putative Branched-chain amino acid ABC transporter, permease protein, at 3,131,919 to 3,132,800
_2749
Position (kb)
3130
3131
3132 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3129.231 kb on + strand, within PP_2746 at 3129.244 kb on - strand, within PP_2746 at 3129.258 kb on + strand, within PP_2746 at 3129.259 kb on - strand, within PP_2746 at 3129.426 kb on + strand, within PP_2746 at 3129.427 kb on - strand, within PP_2746 at 3129.658 kb on - strand, within PP_2746 at 3129.658 kb on - strand, within PP_2746 at 3129.659 kb on + strand, within PP_2746 at 3129.659 kb on + strand, within PP_2746 at 3129.660 kb on - strand, within PP_2746 at 3129.660 kb on - strand at 3129.666 kb on + strand, within PP_2746 at 3129.746 kb on + strand, within PP_2746 at 3129.752 kb on + strand, within PP_2746 at 3129.753 kb on - strand, within PP_2746 at 3129.842 kb on + strand, within PP_2746 at 3129.842 kb on + strand, within PP_2746 at 3130.018 kb on - strand, within PP_2746 at 3130.191 kb on + strand at 3130.191 kb on + strand at 3130.192 kb on - strand at 3130.192 kb on - strand at 3130.192 kb on - strand at 3130.192 kb on - strand at 3130.221 kb on + strand at 3130.570 kb on - strand, within PP_2747 at 3130.807 kb on - strand at 3130.825 kb on - strand, within PP_2747 at 3131.080 kb on + strand at 3131.081 kb on - strand at 3131.081 kb on - strand at 3131.081 kb on - strand at 3131.081 kb on - strand at 3131.101 kb on - strand at 3131.135 kb on + strand at 3131.312 kb on - strand, within PP_2748 at 3131.615 kb on - strand, within PP_2748 at 3131.841 kb on + strand, within PP_2748 at 3131.841 kb on + strand, within PP_2748 at 3132.003 kb on + strand at 3132.003 kb on + strand at 3132.122 kb on - strand, within PP_2749
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 3,129,231 + PP_2746 0.34 -2.1 3,129,244 - PP_2746 0.35 -0.8 3,129,258 + PP_2746 0.36 -1.2 3,129,259 - PP_2746 0.36 +0.9 3,129,426 + PP_2746 0.48 +0.2 3,129,427 - PP_2746 0.48 -1.1 3,129,658 - PP_2746 0.64 +0.5 3,129,658 - PP_2746 0.64 -0.7 3,129,659 + PP_2746 0.64 -0.1 3,129,659 + PP_2746 0.64 +0.5 3,129,660 - PP_2746 0.64 +1.1 3,129,660 - -0.9 3,129,666 + PP_2746 0.65 +0.3 3,129,746 + PP_2746 0.70 +0.2 3,129,752 + PP_2746 0.71 +0.7 3,129,753 - PP_2746 0.71 -0.2 3,129,842 + PP_2746 0.77 +0.2 3,129,842 + PP_2746 0.77 +1.3 3,130,018 - PP_2746 0.90 +0.6 3,130,191 + -0.5 3,130,191 + +0.1 3,130,192 - -0.8 3,130,192 - -0.2 3,130,192 - +0.5 3,130,192 - -0.7 3,130,221 + +0.1 3,130,570 - PP_2747 0.40 +0.6 3,130,807 - +2.1 3,130,825 - PP_2747 0.66 +0.6 3,131,080 + -1.9 3,131,081 - -0.2 3,131,081 - -1.6 3,131,081 - -0.0 3,131,081 - -1.7 3,131,101 - +1.6 3,131,135 + +0.9 3,131,312 - PP_2748 0.24 -0.1 3,131,615 - PP_2748 0.62 +1.5 3,131,841 + PP_2748 0.90 -0.8 3,131,841 + PP_2748 0.90 -0.4 3,132,003 + +0.8 3,132,003 + -0.8 3,132,122 - PP_2749 0.23 -0.5
Or see this region's nucleotide sequence