Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1823 and PP_1824 are separated by 43 nucleotides PP_1824 and PP_1825 are separated by 174 nucleotides PP_1825 and PP_1826 are separated by 85 nucleotides PP_1826 and PP_1827 are separated by 181 nucleotides
PP_1823: PP_1823 - GTP cyclohydrolase 1, at 2,049,331 to 2,049,903
_1823
PP_1824: PP_1824 - Smr domain protein, at 2,049,947 to 2,050,507
_1824
PP_1825: PP_1825 - conserved exported protein of unknown function, at 2,050,682 to 2,051,002
_1825
PP_1826: PP_1826 - Hydrolase, isochorismatase family, at 2,051,088 to 2,051,678
_1826
PP_1827: PP_1827 - N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase, at 2,051,860 to 2,052,768
_1827
Position (kb)
2050
2051
2052 Strain fitness (log2 ratio)
-2
-1
0
1 at 2049.990 kb on + strand at 2049.990 kb on + strand at 2049.990 kb on + strand at 2050.316 kb on + strand, within PP_1824 at 2050.316 kb on + strand, within PP_1824 at 2050.461 kb on - strand at 2050.521 kb on - strand at 2050.593 kb on + strand at 2050.717 kb on + strand, within PP_1825 at 2050.718 kb on - strand, within PP_1825 at 2050.851 kb on + strand, within PP_1825 at 2050.852 kb on - strand, within PP_1825 at 2050.893 kb on - strand, within PP_1825 at 2050.948 kb on + strand, within PP_1825 at 2050.993 kb on + strand at 2050.994 kb on - strand at 2050.994 kb on - strand at 2050.994 kb on - strand at 2050.994 kb on - strand at 2051.243 kb on - strand, within PP_1826 at 2051.501 kb on + strand, within PP_1826 at 2051.579 kb on - strand, within PP_1826 at 2051.579 kb on - strand, within PP_1826 at 2051.579 kb on - strand, within PP_1826 at 2051.635 kb on + strand at 2051.705 kb on + strand at 2051.705 kb on + strand at 2051.706 kb on - strand at 2051.706 kb on - strand at 2051.706 kb on - strand at 2051.707 kb on + strand at 2051.707 kb on + strand at 2051.707 kb on + strand at 2051.707 kb on + strand at 2051.707 kb on + strand at 2051.711 kb on + strand at 2051.711 kb on + strand at 2051.711 kb on + strand at 2051.712 kb on - strand at 2051.712 kb on - strand at 2051.712 kb on - strand at 2051.712 kb on - strand at 2051.712 kb on - strand at 2051.712 kb on - strand at 2051.825 kb on + strand at 2051.855 kb on - strand at 2051.855 kb on - strand at 2051.855 kb on - strand at 2051.893 kb on + strand at 2051.894 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 2,049,990 + +0.2 2,049,990 + -0.8 2,049,990 + +0.6 2,050,316 + PP_1824 0.66 +1.4 2,050,316 + PP_1824 0.66 +0.7 2,050,461 - -1.9 2,050,521 - +0.2 2,050,593 + -0.5 2,050,717 + PP_1825 0.11 +0.5 2,050,718 - PP_1825 0.11 +1.0 2,050,851 + PP_1825 0.53 -0.1 2,050,852 - PP_1825 0.53 -0.7 2,050,893 - PP_1825 0.66 +0.9 2,050,948 + PP_1825 0.83 +1.2 2,050,993 + -1.3 2,050,994 - -0.0 2,050,994 - +0.3 2,050,994 - +0.5 2,050,994 - +0.1 2,051,243 - PP_1826 0.26 -0.3 2,051,501 + PP_1826 0.70 -0.2 2,051,579 - PP_1826 0.83 +1.0 2,051,579 - PP_1826 0.83 +0.3 2,051,579 - PP_1826 0.83 +1.0 2,051,635 + -0.4 2,051,705 + -0.1 2,051,705 + -0.3 2,051,706 - -0.3 2,051,706 - -0.1 2,051,706 - -0.2 2,051,707 + +0.8 2,051,707 + +0.4 2,051,707 + +1.0 2,051,707 + +0.6 2,051,707 + -1.8 2,051,711 + -2.2 2,051,711 + -1.5 2,051,711 + -1.1 2,051,712 - -0.8 2,051,712 - +0.4 2,051,712 - +0.2 2,051,712 - +0.7 2,051,712 - +0.0 2,051,712 - -0.4 2,051,825 + -0.3 2,051,855 - +1.2 2,051,855 - +0.6 2,051,855 - +0.1 2,051,893 + +0.4 2,051,894 - +0.5
Or see this region's nucleotide sequence