Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1086 and PP_1087 are separated by 118 nucleotides PP_1087 and PP_1088 are separated by 370 nucleotides
PP_1086: PP_1086 - dihydroorotase, at 1,244,481 to 1,245,527
_1086
PP_1087: PP_1087 - Outer membrane protein, OmpA family, at 1,245,646 to 1,246,599
_1087
PP_1088: PP_1088 - Argininosuccinate synthase, at 1,246,970 to 1,248,187
_1088
Position (kb)
1245
1246
1247 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1245.593 kb on + strand at 1245.630 kb on + strand at 1245.671 kb on - strand at 1245.813 kb on + strand, within PP_1087 at 1245.814 kb on - strand, within PP_1087 at 1246.035 kb on + strand, within PP_1087 at 1246.035 kb on + strand, within PP_1087 at 1246.036 kb on - strand, within PP_1087 at 1246.054 kb on + strand, within PP_1087 at 1246.055 kb on - strand, within PP_1087 at 1246.055 kb on - strand, within PP_1087 at 1246.118 kb on - strand, within PP_1087 at 1246.118 kb on - strand, within PP_1087 at 1246.118 kb on - strand, within PP_1087 at 1246.126 kb on + strand, within PP_1087 at 1246.162 kb on + strand, within PP_1087 at 1246.163 kb on - strand, within PP_1087 at 1246.163 kb on - strand, within PP_1087 at 1246.255 kb on + strand, within PP_1087 at 1246.255 kb on + strand, within PP_1087 at 1246.255 kb on + strand, within PP_1087 at 1246.256 kb on - strand, within PP_1087 at 1246.256 kb on - strand, within PP_1087 at 1246.256 kb on - strand, within PP_1087 at 1246.327 kb on - strand, within PP_1087 at 1246.433 kb on - strand, within PP_1087 at 1246.847 kb on - strand at 1246.909 kb on - strand at 1246.949 kb on - strand at 1246.949 kb on - strand at 1246.993 kb on - strand at 1246.993 kb on - strand at 1246.993 kb on - strand at 1247.017 kb on + strand at 1247.017 kb on + strand at 1247.047 kb on + strand at 1247.047 kb on + strand at 1247.051 kb on + strand at 1247.051 kb on + strand at 1247.051 kb on + strand at 1247.051 kb on + strand at 1247.052 kb on - strand at 1247.052 kb on - strand at 1247.052 kb on - strand at 1247.052 kb on - strand at 1247.052 kb on - strand at 1247.150 kb on + strand, within PP_1088 at 1247.150 kb on + strand, within PP_1088 at 1247.150 kb on + strand, within PP_1088 at 1247.151 kb on - strand, within PP_1088 at 1247.151 kb on - strand, within PP_1088 at 1247.151 kb on - strand, within PP_1088 at 1247.151 kb on - strand, within PP_1088 at 1247.369 kb on + strand, within PP_1088 at 1247.369 kb on + strand, within PP_1088 at 1247.369 kb on + strand, within PP_1088 at 1247.370 kb on - strand, within PP_1088 at 1247.447 kb on + strand, within PP_1088 at 1247.447 kb on + strand, within PP_1088 at 1247.448 kb on - strand, within PP_1088 at 1247.448 kb on - strand, within PP_1088 at 1247.534 kb on + strand, within PP_1088 at 1247.535 kb on - strand, within PP_1088 at 1247.557 kb on + strand, within PP_1088
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 1,245,593 + +0.2 1,245,630 + -0.0 1,245,671 - -0.2 1,245,813 + PP_1087 0.18 +0.7 1,245,814 - PP_1087 0.18 +0.7 1,246,035 + PP_1087 0.41 +0.6 1,246,035 + PP_1087 0.41 +0.7 1,246,036 - PP_1087 0.41 +0.5 1,246,054 + PP_1087 0.43 -0.2 1,246,055 - PP_1087 0.43 +0.3 1,246,055 - PP_1087 0.43 +0.3 1,246,118 - PP_1087 0.49 +0.7 1,246,118 - PP_1087 0.49 +0.4 1,246,118 - PP_1087 0.49 +0.0 1,246,126 + PP_1087 0.50 +2.7 1,246,162 + PP_1087 0.54 +0.0 1,246,163 - PP_1087 0.54 +0.5 1,246,163 - PP_1087 0.54 +1.3 1,246,255 + PP_1087 0.64 +0.6 1,246,255 + PP_1087 0.64 +0.1 1,246,255 + PP_1087 0.64 +0.5 1,246,256 - PP_1087 0.64 -0.9 1,246,256 - PP_1087 0.64 +0.5 1,246,256 - PP_1087 0.64 -2.2 1,246,327 - PP_1087 0.71 +1.4 1,246,433 - PP_1087 0.82 +0.5 1,246,847 - +1.2 1,246,909 - -0.8 1,246,949 - -2.6 1,246,949 - -0.2 1,246,993 - -0.8 1,246,993 - -0.8 1,246,993 - -1.7 1,247,017 + -0.2 1,247,017 + -0.5 1,247,047 + -2.0 1,247,047 + -1.6 1,247,051 + +0.0 1,247,051 + -0.4 1,247,051 + -0.5 1,247,051 + -0.6 1,247,052 - -1.1 1,247,052 - -0.6 1,247,052 - -0.8 1,247,052 - -1.1 1,247,052 - -0.0 1,247,150 + PP_1088 0.15 -1.5 1,247,150 + PP_1088 0.15 -1.0 1,247,150 + PP_1088 0.15 -0.7 1,247,151 - PP_1088 0.15 -0.4 1,247,151 - PP_1088 0.15 +0.1 1,247,151 - PP_1088 0.15 -3.3 1,247,151 - PP_1088 0.15 -1.3 1,247,369 + PP_1088 0.33 -1.0 1,247,369 + PP_1088 0.33 -2.6 1,247,369 + PP_1088 0.33 -1.8 1,247,370 - PP_1088 0.33 -0.2 1,247,447 + PP_1088 0.39 -0.7 1,247,447 + PP_1088 0.39 -4.2 1,247,448 - PP_1088 0.39 -2.3 1,247,448 - PP_1088 0.39 -1.1 1,247,534 + PP_1088 0.46 -1.4 1,247,535 - PP_1088 0.46 -2.7 1,247,557 + PP_1088 0.48 -2.6
Or see this region's nucleotide sequence