Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1067 and PP_1068 are separated by 65 nucleotides PP_1068 and PP_1069 overlap by 40 nucleotides PP_1069 and PP_1070 are separated by 0 nucleotides PP_1070 and PP_1071 are separated by 145 nucleotides
PP_1067: PP_1067 - Sensor histidine kinase, at 1,221,302 to 1,223,209
_1067
PP_1068: PP_1068 - glutamate / aspartate ABC transporter - ATP binding subunit, at 1,223,275 to 1,224,045
_1068
PP_1069: PP_1069 - glutamate / aspartate ABC transporter - permease subunit, at 1,224,006 to 1,224,677
_1069
PP_1070: PP_1070 - glutamate / aspartate ABC transporter - permease subunit, at 1,224,678 to 1,225,424
_1070
PP_1071: PP_1071 - glutamate / aspartate ABC transporter - periplasmic binding protein, at 1,225,570 to 1,226,490
_1071
Position (kb)
1224
1225 Strain fitness (log2 ratio)
-1
0
1
2 at 1223.085 kb on + strand at 1223.085 kb on + strand at 1223.133 kb on - strand at 1223.221 kb on + strand at 1223.222 kb on - strand at 1223.435 kb on - strand, within PP_1068 at 1223.520 kb on + strand, within PP_1068 at 1223.521 kb on - strand, within PP_1068 at 1223.521 kb on - strand, within PP_1068 at 1223.639 kb on - strand, within PP_1068 at 1224.070 kb on + strand at 1224.166 kb on + strand, within PP_1069 at 1224.166 kb on + strand, within PP_1069 at 1224.167 kb on - strand, within PP_1069 at 1224.167 kb on - strand, within PP_1069 at 1224.168 kb on + strand, within PP_1069 at 1224.271 kb on + strand, within PP_1069 at 1224.272 kb on - strand, within PP_1069 at 1224.410 kb on + strand, within PP_1069 at 1224.454 kb on + strand, within PP_1069 at 1224.454 kb on + strand, within PP_1069 at 1224.455 kb on - strand at 1224.497 kb on - strand, within PP_1069 at 1224.497 kb on - strand, within PP_1069 at 1224.680 kb on - strand at 1224.769 kb on + strand, within PP_1070 at 1224.770 kb on - strand, within PP_1070 at 1224.770 kb on - strand, within PP_1070 at 1224.770 kb on - strand, within PP_1070 at 1224.770 kb on - strand, within PP_1070 at 1224.958 kb on + strand, within PP_1070 at 1224.958 kb on + strand, within PP_1070 at 1224.959 kb on - strand, within PP_1070 at 1224.959 kb on - strand, within PP_1070 at 1224.959 kb on - strand, within PP_1070 at 1225.172 kb on - strand, within PP_1070 at 1225.223 kb on - strand, within PP_1070 at 1225.351 kb on + strand at 1225.352 kb on - strand at 1225.363 kb on + strand at 1225.418 kb on - strand at 1225.418 kb on - strand at 1225.502 kb on + strand at 1225.503 kb on - strand at 1225.503 kb on - strand at 1225.509 kb on + strand at 1225.557 kb on + strand at 1225.557 kb on + strand at 1225.561 kb on + strand at 1225.561 kb on + strand at 1225.562 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 1,223,085 + +0.1 1,223,085 + +0.4 1,223,133 - +0.4 1,223,221 + -0.4 1,223,222 - -0.0 1,223,435 - PP_1068 0.21 -0.2 1,223,520 + PP_1068 0.32 +0.1 1,223,521 - PP_1068 0.32 -0.1 1,223,521 - PP_1068 0.32 +0.7 1,223,639 - PP_1068 0.47 -1.6 1,224,070 + -0.8 1,224,166 + PP_1069 0.24 +0.4 1,224,166 + PP_1069 0.24 -0.2 1,224,167 - PP_1069 0.24 -0.6 1,224,167 - PP_1069 0.24 +0.9 1,224,168 + PP_1069 0.24 +2.1 1,224,271 + PP_1069 0.39 -0.0 1,224,272 - PP_1069 0.40 +1.8 1,224,410 + PP_1069 0.60 +1.0 1,224,454 + PP_1069 0.67 +0.9 1,224,454 + PP_1069 0.67 +0.0 1,224,455 - +1.2 1,224,497 - PP_1069 0.73 +1.0 1,224,497 - PP_1069 0.73 +0.5 1,224,680 - +0.6 1,224,769 + PP_1070 0.12 +0.2 1,224,770 - PP_1070 0.12 +0.4 1,224,770 - PP_1070 0.12 +0.5 1,224,770 - PP_1070 0.12 -0.6 1,224,770 - PP_1070 0.12 +1.4 1,224,958 + PP_1070 0.37 +1.2 1,224,958 + PP_1070 0.37 +0.5 1,224,959 - PP_1070 0.38 -0.2 1,224,959 - PP_1070 0.38 +0.5 1,224,959 - PP_1070 0.38 +0.0 1,225,172 - PP_1070 0.66 -0.2 1,225,223 - PP_1070 0.73 +0.4 1,225,351 + +0.7 1,225,352 - +1.6 1,225,363 + -0.4 1,225,418 - +0.5 1,225,418 - +1.5 1,225,502 + -0.4 1,225,503 - +1.4 1,225,503 - -1.3 1,225,509 + +0.1 1,225,557 + +0.7 1,225,557 + +0.2 1,225,561 + +0.4 1,225,561 + +0.3 1,225,562 - +0.6
Or see this region's nucleotide sequence