Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0983 and PP_0984 are separated by 146 nucleotides PP_0984 and PP_0986 are separated by 656 nucleotides
PP_0983: PP_0983 - conserved membrane protein of unknown function, at 1,121,668 to 1,122,729
_0983
PP_0984: PP_0984 - conserved membrane protein of unknown function, at 1,122,876 to 1,123,364
_0984
PP_0986: PP_0986 - aminomethyltransferase, at 1,124,021 to 1,125,142
_0986
Position (kb)
1122
1123
1124 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1122.730 kb on + strand at 1122.730 kb on + strand at 1122.731 kb on - strand at 1122.821 kb on - strand at 1122.878 kb on - strand at 1122.878 kb on - strand at 1122.878 kb on - strand at 1122.919 kb on + strand at 1122.920 kb on - strand at 1122.920 kb on - strand at 1123.074 kb on + strand, within PP_0984 at 1123.074 kb on + strand, within PP_0984 at 1123.075 kb on - strand, within PP_0984 at 1123.147 kb on - strand, within PP_0984 at 1123.147 kb on - strand, within PP_0984 at 1123.152 kb on - strand, within PP_0984 at 1123.240 kb on + strand, within PP_0984 at 1123.279 kb on - strand, within PP_0984 at 1123.288 kb on + strand, within PP_0984 at 1123.289 kb on - strand, within PP_0984 at 1123.350 kb on + strand at 1123.350 kb on + strand at 1123.351 kb on - strand at 1123.392 kb on + strand at 1123.393 kb on - strand at 1123.393 kb on - strand at 1123.398 kb on - strand at 1123.669 kb on + strand at 1123.669 kb on + strand at 1123.669 kb on + strand at 1123.677 kb on - strand at 1123.702 kb on + strand at 1123.754 kb on + strand at 1123.776 kb on - strand at 1123.776 kb on - strand at 1123.880 kb on + strand at 1123.880 kb on + strand at 1123.962 kb on + strand at 1123.962 kb on + strand at 1123.986 kb on - strand at 1123.986 kb on - strand at 1123.986 kb on - strand at 1123.991 kb on + strand at 1123.991 kb on + strand at 1123.992 kb on - strand at 1124.031 kb on + strand at 1124.034 kb on + strand at 1124.034 kb on + strand at 1124.035 kb on - strand at 1124.035 kb on - strand at 1124.035 kb on - strand at 1124.035 kb on - strand at 1124.137 kb on + strand, within PP_0986 at 1124.138 kb on - strand, within PP_0986 at 1124.138 kb on - strand, within PP_0986 at 1124.138 kb on - strand, within PP_0986 at 1124.138 kb on - strand, within PP_0986 at 1124.140 kb on - strand, within PP_0986 at 1124.140 kb on - strand, within PP_0986 at 1124.248 kb on + strand, within PP_0986
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 1,122,730 + +0.2 1,122,730 + -1.4 1,122,731 - -0.4 1,122,821 - +0.3 1,122,878 - -0.0 1,122,878 - +0.5 1,122,878 - -0.7 1,122,919 + +2.5 1,122,920 - +0.6 1,122,920 - +1.2 1,123,074 + PP_0984 0.40 +0.9 1,123,074 + PP_0984 0.40 +1.0 1,123,075 - PP_0984 0.41 -1.5 1,123,147 - PP_0984 0.55 +0.6 1,123,147 - PP_0984 0.55 +2.1 1,123,152 - PP_0984 0.56 +1.6 1,123,240 + PP_0984 0.74 -0.5 1,123,279 - PP_0984 0.82 +0.1 1,123,288 + PP_0984 0.84 +0.4 1,123,289 - PP_0984 0.84 +0.2 1,123,350 + +1.1 1,123,350 + +1.2 1,123,351 - +0.7 1,123,392 + +0.8 1,123,393 - +1.0 1,123,393 - -0.1 1,123,398 - +1.4 1,123,669 + -0.2 1,123,669 + -1.6 1,123,669 + +1.1 1,123,677 - +0.9 1,123,702 + +0.5 1,123,754 + -0.3 1,123,776 - +0.6 1,123,776 - -0.0 1,123,880 + +0.2 1,123,880 + +0.8 1,123,962 + -0.7 1,123,962 + +0.2 1,123,986 - -1.8 1,123,986 - -0.3 1,123,986 - +0.6 1,123,991 + +2.3 1,123,991 + +0.2 1,123,992 - -0.3 1,124,031 + -0.5 1,124,034 + +1.9 1,124,034 + +0.4 1,124,035 - -1.0 1,124,035 - -0.6 1,124,035 - +0.3 1,124,035 - -0.4 1,124,137 + PP_0986 0.10 -0.7 1,124,138 - PP_0986 0.10 +0.5 1,124,138 - PP_0986 0.10 +0.6 1,124,138 - PP_0986 0.10 +0.2 1,124,138 - PP_0986 0.10 +0.7 1,124,140 - PP_0986 0.11 -0.3 1,124,140 - PP_0986 0.11 +0.0 1,124,248 + PP_0986 0.20 +0.8
Or see this region's nucleotide sequence