Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0289 and PP_0290 are separated by 0 nucleotides PP_0290 and PP_0291 are separated by 0 nucleotides PP_0291 and PP_0292 are separated by 27 nucleotides PP_0292 and PP_0293 are separated by 10 nucleotides PP_0293 and PP_0294 are separated by 117 nucleotides
PP_0289: PP_0289 - Imidazoleglycerol-phosphate dehydratase, at 349,955 to 350,587
_0289
PP_0290: PP_0290 - Imidazole glycerol phosphate synthase subunit HisH, at 350,588 to 351,226
_0290
PP_0291: PP_0291 - conserved protein of unknown function, at 351,227 to 351,487
_0291
PP_0292: PP_0292 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) , at 351,515 to 352,252
_0292
PP_0293: PP_0293 - Imidazole glycerol phosphate synthase subunit HisF, at 352,263 to 353,033
_0293
PP_0294: PP_0294 - Choline / carnitine / glycine betaine ABC transporter, ATPase component (from data) , at 353,151 to 354,329
_0294
Position (kb)
351
352
353 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 350.548 kb on + strand at 350.548 kb on + strand at 350.548 kb on + strand at 350.549 kb on - strand at 350.585 kb on + strand at 350.612 kb on + strand at 350.658 kb on + strand, within PP_0290 at 350.658 kb on + strand, within PP_0290 at 350.659 kb on - strand, within PP_0290 at 350.659 kb on - strand, within PP_0290 at 350.668 kb on + strand, within PP_0290 at 350.680 kb on + strand, within PP_0290 at 351.035 kb on + strand, within PP_0290 at 351.050 kb on + strand, within PP_0290 at 351.050 kb on + strand, within PP_0290 at 351.051 kb on - strand, within PP_0290 at 351.051 kb on - strand, within PP_0290 at 351.051 kb on - strand, within PP_0290 at 351.053 kb on + strand, within PP_0290 at 351.053 kb on + strand, within PP_0290 at 351.053 kb on + strand, within PP_0290 at 351.054 kb on - strand, within PP_0290 at 351.054 kb on - strand, within PP_0290 at 351.098 kb on + strand, within PP_0290 at 351.099 kb on - strand, within PP_0290 at 351.224 kb on + strand at 351.224 kb on + strand at 351.225 kb on - strand at 351.396 kb on - strand, within PP_0291 at 351.396 kb on - strand, within PP_0291 at 351.398 kb on + strand, within PP_0291 at 351.398 kb on + strand, within PP_0291 at 351.399 kb on - strand, within PP_0291 at 351.399 kb on - strand, within PP_0291 at 351.399 kb on - strand, within PP_0291 at 351.399 kb on - strand, within PP_0291 at 351.499 kb on + strand at 351.499 kb on + strand at 351.523 kb on + strand at 351.524 kb on - strand at 351.590 kb on - strand, within PP_0292 at 351.791 kb on + strand, within PP_0292 at 351.792 kb on - strand, within PP_0292 at 351.792 kb on - strand, within PP_0292 at 351.792 kb on - strand, within PP_0292 at 351.812 kb on + strand at 351.813 kb on - strand, within PP_0292 at 351.813 kb on - strand, within PP_0292 at 352.193 kb on + strand at 352.193 kb on + strand at 352.194 kb on - strand at 352.194 kb on - strand at 352.194 kb on - strand at 352.194 kb on - strand at 352.241 kb on + strand at 352.242 kb on - strand at 352.242 kb on - strand at 352.242 kb on - strand at 352.242 kb on - strand at 352.242 kb on - strand at 352.242 kb on - strand at 352.457 kb on + strand, within PP_0293 at 352.457 kb on + strand, within PP_0293 at 352.457 kb on + strand, within PP_0293 at 352.977 kb on + strand at 352.977 kb on + strand at 352.978 kb on - strand at 353.001 kb on + strand at 353.002 kb on - strand at 353.002 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 350,548 + +1.4 350,548 + -1.2 350,548 + -0.1 350,549 - +0.7 350,585 + +0.5 350,612 + +1.3 350,658 + PP_0290 0.11 -0.7 350,658 + PP_0290 0.11 -0.3 350,659 - PP_0290 0.11 +0.4 350,659 - PP_0290 0.11 +0.2 350,668 + PP_0290 0.13 +0.4 350,680 + PP_0290 0.14 -1.1 351,035 + PP_0290 0.70 -0.8 351,050 + PP_0290 0.72 +0.1 351,050 + PP_0290 0.72 -0.8 351,051 - PP_0290 0.72 +0.2 351,051 - PP_0290 0.72 +0.3 351,051 - PP_0290 0.72 +0.7 351,053 + PP_0290 0.73 -0.4 351,053 + PP_0290 0.73 +1.1 351,053 + PP_0290 0.73 -0.2 351,054 - PP_0290 0.73 +0.3 351,054 - PP_0290 0.73 +0.7 351,098 + PP_0290 0.80 -0.5 351,099 - PP_0290 0.80 +0.9 351,224 + +0.5 351,224 + +0.6 351,225 - -0.6 351,396 - PP_0291 0.65 -0.8 351,396 - PP_0291 0.65 -0.4 351,398 + PP_0291 0.66 +0.5 351,398 + PP_0291 0.66 +0.5 351,399 - PP_0291 0.66 +0.8 351,399 - PP_0291 0.66 -0.6 351,399 - PP_0291 0.66 +0.3 351,399 - PP_0291 0.66 +0.4 351,499 + -2.1 351,499 + -0.5 351,523 + -0.2 351,524 - +0.4 351,590 - PP_0292 0.10 +1.2 351,791 + PP_0292 0.37 +0.6 351,792 - PP_0292 0.38 +0.1 351,792 - PP_0292 0.38 -0.9 351,792 - PP_0292 0.38 +1.4 351,812 + -1.5 351,813 - PP_0292 0.40 +0.8 351,813 - PP_0292 0.40 -0.3 352,193 + +0.1 352,193 + +0.3 352,194 - +0.2 352,194 - -0.3 352,194 - -0.3 352,194 - +1.1 352,241 + +0.3 352,242 - -0.9 352,242 - +0.1 352,242 - -0.9 352,242 - +0.4 352,242 - +0.6 352,242 - -0.6 352,457 + PP_0293 0.25 -0.1 352,457 + PP_0293 0.25 -0.2 352,457 + PP_0293 0.25 +0.2 352,977 + +2.3 352,977 + -1.2 352,978 - -0.3 353,001 + -0.4 353,002 - +0.8 353,002 - +0.4
Or see this region's nucleotide sequence