Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0189 and PP_0190 are separated by 222 nucleotides PP_0190 and PP_0191 are separated by 231 nucleotides PP_0191 and PP_0192 are separated by 8 nucleotides
PP_0189: PP_0189 - putative heme biosynthesis protein, at 240,830 to 242,068
_0189
PP_0190: PP_0190 - putative disulfide bond formation protein B, at 242,291 to 242,797
_0190
PP_0191: PP_0191 - Transcriptional regulatory protein AlgQ, at 243,029 to 243,499
_0191
PP_0192: PP_0192 - putative FKBP-type 25 kDa peptidyl-prolyl cis-trans isomerase, at 243,508 to 244,170
_0192
Position (kb) 242
243 Strain fitness (log2 ratio) -2
-1
0
1
2 at 241.421 kb on + strand, within PP_0189 at 241.421 kb on + strand, within PP_0189 at 241.421 kb on + strand, within PP_0189 at 241.422 kb on - strand, within PP_0189 at 241.422 kb on - strand, within PP_0189 at 241.563 kb on + strand, within PP_0189 at 241.653 kb on - strand, within PP_0189 at 241.674 kb on + strand, within PP_0189 at 241.675 kb on - strand, within PP_0189 at 241.728 kb on - strand, within PP_0189 at 241.896 kb on - strand, within PP_0189 at 242.112 kb on + strand at 242.117 kb on + strand at 242.166 kb on + strand at 242.173 kb on + strand at 242.174 kb on - strand at 242.349 kb on + strand, within PP_0190 at 242.349 kb on + strand, within PP_0190 at 242.350 kb on - strand, within PP_0190 at 242.350 kb on - strand, within PP_0190 at 242.496 kb on - strand at 242.587 kb on - strand, within PP_0190 at 242.587 kb on - strand, within PP_0190 at 242.587 kb on - strand, within PP_0190 at 242.587 kb on - strand, within PP_0190 at 242.587 kb on - strand, within PP_0190 at 242.768 kb on + strand at 242.840 kb on - strand at 242.840 kb on - strand at 242.840 kb on - strand at 242.847 kb on + strand at 242.850 kb on + strand at 242.860 kb on + strand at 242.880 kb on + strand at 242.880 kb on + strand at 242.907 kb on + strand at 242.908 kb on - strand at 242.924 kb on + strand at 243.006 kb on + strand at 243.037 kb on + strand at 243.038 kb on - strand at 243.203 kb on + strand, within PP_0191 at 243.203 kb on + strand, within PP_0191 at 243.319 kb on + strand, within PP_0191 at 243.319 kb on + strand, within PP_0191 at 243.554 kb on + strand at 243.554 kb on + strand at 243.554 kb on + strand at 243.555 kb on - strand at 243.590 kb on + strand, within PP_0192 at 243.590 kb on + strand, within PP_0192 at 243.610 kb on + strand, within PP_0192 at 243.611 kb on - strand, within PP_0192 at 243.743 kb on + strand, within PP_0192 at 243.744 kb on - strand, within PP_0192 at 243.744 kb on - strand, within PP_0192 at 243.744 kb on - strand, within PP_0192
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 241,421 + PP_0189 0.48 +1.0 241,421 + PP_0189 0.48 +0.3 241,421 + PP_0189 0.48 -0.6 241,422 - PP_0189 0.48 +1.2 241,422 - PP_0189 0.48 +0.4 241,563 + PP_0189 0.59 -0.1 241,653 - PP_0189 0.66 -0.2 241,674 + PP_0189 0.68 +0.4 241,675 - PP_0189 0.68 -0.0 241,728 - PP_0189 0.72 -0.1 241,896 - PP_0189 0.86 +0.9 242,112 + +0.4 242,117 + -0.0 242,166 + -0.7 242,173 + +0.1 242,174 - +0.8 242,349 + PP_0190 0.11 -0.1 242,349 + PP_0190 0.11 +1.2 242,350 - PP_0190 0.12 +1.0 242,350 - PP_0190 0.12 +0.3 242,496 - +1.9 242,587 - PP_0190 0.58 +0.9 242,587 - PP_0190 0.58 -0.0 242,587 - PP_0190 0.58 +0.6 242,587 - PP_0190 0.58 +0.6 242,587 - PP_0190 0.58 +0.4 242,768 + -0.1 242,840 - +0.4 242,840 - +0.0 242,840 - -1.3 242,847 + -1.9 242,850 + -0.1 242,860 + +0.5 242,880 + -1.2 242,880 + -0.4 242,907 + -1.2 242,908 - +0.5 242,924 + -1.4 243,006 + +0.7 243,037 + -0.8 243,038 - +0.1 243,203 + PP_0191 0.37 -0.4 243,203 + PP_0191 0.37 +0.6 243,319 + PP_0191 0.62 +1.4 243,319 + PP_0191 0.62 +0.1 243,554 + +0.8 243,554 + +0.7 243,554 + +0.2 243,555 - +0.6 243,590 + PP_0192 0.12 -0.4 243,590 + PP_0192 0.12 +0.3 243,610 + PP_0192 0.15 +0.3 243,611 - PP_0192 0.16 -0.6 243,743 + PP_0192 0.35 -0.2 243,744 - PP_0192 0.36 +0.3 243,744 - PP_0192 0.36 +0.3 243,744 - PP_0192 0.36 +0.4
Or see this region's nucleotide sequence