Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0167 and PP_5429 are separated by 63 nucleotides PP_5429 and PP_5430 are separated by 85 nucleotides PP_5430 and PP_0168 are separated by 409 nucleotides
PP_0167: PP_0167 - Toxin secretion ATP-binding protein, at 191,361 to 193,517
_0167
PP_5429: PP_5429 - protein of unknown function, at 193,581 to 193,796
_5429
PP_5430: PP_5430 - protein of unknown function, at 193,882 to 194,085
_5430
PP_0168: PP_0168 - putative surface adhesion protein, at 194,495 to 220,543
_0168
Position (kb) 193
194
195 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 192.882 kb on - strand, within PP_0167 at 192.937 kb on + strand, within PP_0167 at 192.938 kb on - strand, within PP_0167 at 193.009 kb on - strand, within PP_0167 at 193.012 kb on + strand, within PP_0167 at 193.012 kb on + strand, within PP_0167 at 193.012 kb on + strand, within PP_0167 at 193.012 kb on + strand, within PP_0167 at 193.012 kb on + strand, within PP_0167 at 193.013 kb on - strand, within PP_0167 at 193.013 kb on - strand, within PP_0167 at 193.013 kb on - strand, within PP_0167 at 193.013 kb on - strand, within PP_0167 at 193.013 kb on - strand, within PP_0167 at 193.013 kb on - strand, within PP_0167 at 193.013 kb on - strand, within PP_0167 at 193.129 kb on + strand, within PP_0167 at 193.137 kb on + strand, within PP_0167 at 193.137 kb on + strand, within PP_0167 at 193.189 kb on - strand, within PP_0167 at 193.189 kb on - strand, within PP_0167 at 193.381 kb on - strand at 193.446 kb on - strand at 193.620 kb on - strand, within PP_5429 at 193.626 kb on + strand, within PP_5429 at 193.626 kb on + strand, within PP_5429 at 193.626 kb on + strand, within PP_5429 at 193.627 kb on - strand, within PP_5429 at 193.682 kb on + strand, within PP_5429 at 193.692 kb on + strand, within PP_5429 at 193.692 kb on + strand, within PP_5429 at 193.693 kb on - strand, within PP_5429 at 193.693 kb on - strand, within PP_5429 at 193.773 kb on + strand, within PP_5429 at 193.819 kb on + strand at 193.820 kb on - strand at 193.833 kb on - strand at 193.858 kb on + strand at 193.858 kb on + strand at 193.858 kb on + strand at 193.859 kb on - strand at 193.859 kb on - strand at 193.887 kb on + strand at 193.888 kb on - strand at 193.888 kb on - strand at 193.889 kb on + strand at 193.889 kb on + strand at 193.890 kb on - strand at 193.890 kb on - strand at 193.997 kb on + strand, within PP_5430 at 193.997 kb on + strand, within PP_5430 at 193.998 kb on - strand, within PP_5430 at 194.025 kb on + strand, within PP_5430 at 194.026 kb on - strand, within PP_5430 at 194.096 kb on + strand at 194.096 kb on + strand at 194.138 kb on - strand at 194.150 kb on - strand at 194.192 kb on - strand at 194.192 kb on - strand at 194.208 kb on - strand at 194.208 kb on - strand at 194.221 kb on - strand at 194.235 kb on - strand at 194.253 kb on + strand at 194.254 kb on - strand at 194.254 kb on - strand at 194.254 kb on - strand at 194.283 kb on + strand at 194.284 kb on - strand at 194.284 kb on - strand at 194.284 kb on - strand at 194.289 kb on - strand at 194.294 kb on + strand at 194.309 kb on - strand at 194.309 kb on - strand at 194.338 kb on + strand at 194.422 kb on + strand at 194.423 kb on - strand at 194.441 kb on + strand at 194.467 kb on + strand at 194.468 kb on - strand at 194.508 kb on + strand at 194.508 kb on + strand at 194.509 kb on - strand at 194.539 kb on + strand at 194.571 kb on + strand at 194.571 kb on + strand at 194.572 kb on - strand at 194.635 kb on + strand at 194.710 kb on + strand at 194.710 kb on + strand at 194.710 kb on + strand at 194.711 kb on - strand at 194.711 kb on - strand at 194.711 kb on - strand at 194.797 kb on + strand at 194.798 kb on - strand at 194.812 kb on + strand at 194.981 kb on - strand at 194.981 kb on - strand at 194.981 kb on - strand at 195.055 kb on + strand at 195.055 kb on + strand at 195.055 kb on + strand at 195.056 kb on - strand at 195.056 kb on - strand at 195.056 kb on - strand at 195.083 kb on + strand at 195.083 kb on + strand at 195.085 kb on + strand at 195.085 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 192,882 - PP_0167 0.71 -1.0 192,937 + PP_0167 0.73 +1.2 192,938 - PP_0167 0.73 -0.0 193,009 - PP_0167 0.76 -0.0 193,012 + PP_0167 0.77 +0.0 193,012 + PP_0167 0.77 -0.2 193,012 + PP_0167 0.77 +0.4 193,012 + PP_0167 0.77 -0.5 193,012 + PP_0167 0.77 -0.4 193,013 - PP_0167 0.77 -0.1 193,013 - PP_0167 0.77 +0.3 193,013 - PP_0167 0.77 -1.0 193,013 - PP_0167 0.77 +1.0 193,013 - PP_0167 0.77 -0.4 193,013 - PP_0167 0.77 -1.6 193,013 - PP_0167 0.77 +0.9 193,129 + PP_0167 0.82 -0.2 193,137 + PP_0167 0.82 +0.3 193,137 + PP_0167 0.82 +0.5 193,189 - PP_0167 0.85 -0.6 193,189 - PP_0167 0.85 -0.4 193,381 - +0.1 193,446 - -1.4 193,620 - PP_5429 0.18 +0.6 193,626 + PP_5429 0.21 -0.5 193,626 + PP_5429 0.21 -0.0 193,626 + PP_5429 0.21 +0.5 193,627 - PP_5429 0.21 +1.0 193,682 + PP_5429 0.47 +0.5 193,692 + PP_5429 0.51 -0.8 193,692 + PP_5429 0.51 +1.7 193,693 - PP_5429 0.52 -0.2 193,693 - PP_5429 0.52 -0.0 193,773 + PP_5429 0.89 +1.0 193,819 + +0.5 193,820 - -1.2 193,833 - -0.3 193,858 + -0.3 193,858 + +0.8 193,858 + -1.1 193,859 - +0.3 193,859 - +0.4 193,887 + -0.7 193,888 - +0.5 193,888 - -0.3 193,889 + -3.6 193,889 + -0.6 193,890 - -0.2 193,890 - -1.1 193,997 + PP_5430 0.56 -3.2 193,997 + PP_5430 0.56 -0.6 193,998 - PP_5430 0.57 -2.1 194,025 + PP_5430 0.70 -1.6 194,026 - PP_5430 0.71 +0.1 194,096 + -1.7 194,096 + -1.0 194,138 - +0.1 194,150 - -0.9 194,192 - -0.1 194,192 - -0.2 194,208 - +0.5 194,208 - +0.1 194,221 - -0.5 194,235 - -0.5 194,253 + +0.6 194,254 - +0.3 194,254 - +0.1 194,254 - +0.5 194,283 + +0.5 194,284 - +0.1 194,284 - -1.2 194,284 - -0.3 194,289 - -0.2 194,294 + +1.1 194,309 - +0.3 194,309 - -0.3 194,338 + -0.5 194,422 + -2.2 194,423 - -0.7 194,441 + +1.1 194,467 + +0.3 194,468 - -0.8 194,508 + -0.6 194,508 + +0.4 194,509 - +0.6 194,539 + +1.1 194,571 + -0.4 194,571 + -1.0 194,572 - +0.0 194,635 + +0.3 194,710 + +0.7 194,710 + +0.5 194,710 + +0.4 194,711 - +0.4 194,711 - -0.4 194,711 - -1.2 194,797 + +1.1 194,798 - +0.4 194,812 + +0.2 194,981 - +0.2 194,981 - -0.3 194,981 - -0.2 195,055 + -0.0 195,055 + -1.0 195,055 + +0.6 195,056 - -0.0 195,056 - +0.0 195,056 - +0.4 195,083 + -1.0 195,083 + -0.5 195,085 + +0.1 195,085 + +0.3
Or see this region's nucleotide sequence