Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3072 and PP_3073 are separated by 55 nucleotides PP_3073 and PP_3074 are separated by 10 nucleotides PP_3074 and PP_3075 are separated by 225 nucleotides
PP_3072: PP_3072 - Ecotin, at 3,455,983 to 3,456,462
_3072
PP_3073: PP_3073 - 3-hydroxybutyrate dehydrogenase, at 3,456,518 to 3,457,288
_3073
PP_3074: PP_3074 - D-beta-hydroxybutyrate permease, at 3,457,299 to 3,458,690
_3074
PP_3075: PP_3075 - putative transcriptional regulator, at 3,458,916 to 3,460,361
_3075
Position (kb)
3457
3458
3459 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3456.301 kb on - strand, within PP_3072 at 3456.302 kb on + strand, within PP_3072 at 3456.302 kb on + strand, within PP_3072 at 3456.596 kb on + strand, within PP_3073 at 3456.597 kb on - strand, within PP_3073 at 3456.749 kb on + strand, within PP_3073 at 3456.750 kb on - strand, within PP_3073 at 3456.750 kb on - strand, within PP_3073 at 3456.750 kb on - strand, within PP_3073 at 3456.834 kb on + strand, within PP_3073 at 3456.834 kb on + strand, within PP_3073 at 3456.835 kb on - strand, within PP_3073 at 3456.835 kb on - strand, within PP_3073 at 3457.021 kb on + strand, within PP_3073 at 3457.217 kb on + strand at 3457.218 kb on - strand at 3457.300 kb on + strand at 3457.301 kb on - strand at 3457.318 kb on + strand at 3457.384 kb on + strand at 3457.384 kb on + strand at 3457.384 kb on + strand at 3457.384 kb on + strand at 3457.385 kb on - strand at 3457.385 kb on - strand at 3457.385 kb on - strand at 3457.706 kb on - strand, within PP_3074 at 3457.778 kb on + strand, within PP_3074 at 3457.779 kb on - strand, within PP_3074 at 3458.102 kb on - strand, within PP_3074 at 3458.321 kb on - strand, within PP_3074 at 3458.359 kb on + strand, within PP_3074 at 3458.363 kb on + strand, within PP_3074 at 3458.363 kb on + strand, within PP_3074 at 3458.364 kb on - strand, within PP_3074 at 3458.364 kb on - strand, within PP_3074 at 3458.382 kb on + strand, within PP_3074 at 3458.382 kb on + strand, within PP_3074 at 3458.507 kb on - strand, within PP_3074 at 3458.507 kb on - strand, within PP_3074 at 3458.623 kb on - strand at 3458.623 kb on - strand at 3458.759 kb on - strand at 3458.838 kb on + strand at 3459.059 kb on - strand at 3459.267 kb on + strand, within PP_3075 at 3459.268 kb on - strand, within PP_3075 at 3459.346 kb on + strand, within PP_3075 at 3459.349 kb on + strand, within PP_3075 at 3459.349 kb on + strand, within PP_3075
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 3,456,301 - PP_3072 0.66 +0.4 3,456,302 + PP_3072 0.66 -0.4 3,456,302 + PP_3072 0.66 +0.9 3,456,596 + PP_3073 0.10 -0.8 3,456,597 - PP_3073 0.10 +0.4 3,456,749 + PP_3073 0.30 -1.4 3,456,750 - PP_3073 0.30 +0.2 3,456,750 - PP_3073 0.30 -1.5 3,456,750 - PP_3073 0.30 -0.1 3,456,834 + PP_3073 0.41 -0.4 3,456,834 + PP_3073 0.41 -1.1 3,456,835 - PP_3073 0.41 -0.7 3,456,835 - PP_3073 0.41 +1.1 3,457,021 + PP_3073 0.65 +0.3 3,457,217 + -4.2 3,457,218 - -4.5 3,457,300 + -0.2 3,457,301 - -0.2 3,457,318 + +1.3 3,457,384 + -1.3 3,457,384 + -3.4 3,457,384 + +0.6 3,457,384 + -0.4 3,457,385 - +0.8 3,457,385 - -2.5 3,457,385 - +0.3 3,457,706 - PP_3074 0.29 +0.6 3,457,778 + PP_3074 0.34 +0.4 3,457,779 - PP_3074 0.34 +1.1 3,458,102 - PP_3074 0.58 +0.2 3,458,321 - PP_3074 0.73 +0.3 3,458,359 + PP_3074 0.76 +0.4 3,458,363 + PP_3074 0.76 +1.3 3,458,363 + PP_3074 0.76 +1.2 3,458,364 - PP_3074 0.77 -0.1 3,458,364 - PP_3074 0.77 +0.5 3,458,382 + PP_3074 0.78 +1.0 3,458,382 + PP_3074 0.78 +0.8 3,458,507 - PP_3074 0.87 +0.4 3,458,507 - PP_3074 0.87 +0.1 3,458,623 - +0.8 3,458,623 - +1.1 3,458,759 - -0.0 3,458,838 + -0.7 3,459,059 - -1.2 3,459,267 + PP_3075 0.24 -0.8 3,459,268 - PP_3075 0.24 -0.8 3,459,346 + PP_3075 0.30 -1.4 3,459,349 + PP_3075 0.30 -1.1 3,459,349 + PP_3075 0.30 -0.0
Or see this region's nucleotide sequence