Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2301 and PP_2302 are separated by 163 nucleotides PP_2302 and PP_2303 are separated by 152 nucleotides PP_2303 and PP_2304 are separated by 181 nucleotides
PP_2301: PP_2301 - ATP-dependent Clp protease ATP-binding subunit ClpX, at 2,629,044 to 2,630,372
_2301
PP_2302: PP_2302 - DNA-binding, ATP-dependent protease, at 2,630,536 to 2,632,932
_2302
PP_2303: PP_2303 - DNA binding regulator, beta subunit B, at 2,633,085 to 2,633,357
_2303
PP_2304: PP_2304 - putative Peptidyl-prolyl cis-trans isomerase D, at 2,633,539 to 2,635,410
_2304
Position (kb)
2630
2631
2632
2633 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2629.605 kb on + strand, within PP_2301 at 2630.244 kb on + strand at 2630.244 kb on + strand at 2630.244 kb on + strand at 2630.244 kb on + strand at 2630.453 kb on - strand at 2630.477 kb on + strand at 2630.477 kb on + strand at 2630.477 kb on + strand at 2630.477 kb on + strand at 2630.478 kb on - strand at 2630.675 kb on + strand at 2630.675 kb on + strand at 2630.676 kb on - strand at 2630.676 kb on - strand at 2630.676 kb on - strand at 2630.945 kb on - strand, within PP_2302 at 2630.945 kb on - strand, within PP_2302 at 2631.387 kb on + strand, within PP_2302 at 2631.388 kb on - strand, within PP_2302 at 2631.404 kb on - strand, within PP_2302 at 2631.496 kb on + strand, within PP_2302 at 2631.496 kb on + strand, within PP_2302 at 2631.497 kb on - strand, within PP_2302 at 2631.533 kb on - strand, within PP_2302 at 2631.715 kb on + strand, within PP_2302 at 2631.715 kb on + strand, within PP_2302 at 2631.715 kb on + strand, within PP_2302 at 2631.715 kb on + strand, within PP_2302 at 2631.716 kb on - strand, within PP_2302 at 2631.716 kb on - strand, within PP_2302 at 2631.716 kb on - strand, within PP_2302 at 2631.904 kb on + strand, within PP_2302 at 2632.036 kb on + strand, within PP_2302 at 2632.036 kb on + strand, within PP_2302 at 2632.037 kb on - strand, within PP_2302 at 2632.037 kb on - strand, within PP_2302 at 2632.123 kb on + strand, within PP_2302 at 2632.124 kb on - strand, within PP_2302 at 2632.127 kb on - strand, within PP_2302 at 2632.127 kb on - strand, within PP_2302 at 2632.200 kb on + strand, within PP_2302 at 2632.201 kb on - strand, within PP_2302 at 2632.908 kb on + strand at 2632.930 kb on + strand at 2632.930 kb on + strand at 2632.930 kb on + strand at 2632.931 kb on - strand at 2632.981 kb on + strand at 2633.040 kb on + strand at 2633.044 kb on + strand at 2633.044 kb on + strand at 2633.048 kb on + strand at 2633.048 kb on + strand at 2633.048 kb on + strand at 2633.355 kb on + strand at 2633.355 kb on + strand at 2633.355 kb on + strand at 2633.356 kb on - strand at 2633.356 kb on - strand at 2633.440 kb on + strand at 2633.517 kb on + strand at 2633.518 kb on - strand at 2633.655 kb on + strand at 2633.655 kb on + strand at 2633.655 kb on + strand at 2633.655 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 2,629,605 + PP_2301 0.42 +0.1 2,630,244 + -1.2 2,630,244 + -2.1 2,630,244 + -0.2 2,630,244 + -0.4 2,630,453 - +0.0 2,630,477 + -0.2 2,630,477 + +0.0 2,630,477 + -1.5 2,630,477 + +0.6 2,630,478 - +0.3 2,630,675 + -2.3 2,630,675 + -2.3 2,630,676 - -2.0 2,630,676 - -2.1 2,630,676 - -1.8 2,630,945 - PP_2302 0.17 -2.1 2,630,945 - PP_2302 0.17 +0.3 2,631,387 + PP_2302 0.36 +1.8 2,631,388 - PP_2302 0.36 -1.1 2,631,404 - PP_2302 0.36 -0.7 2,631,496 + PP_2302 0.40 -2.0 2,631,496 + PP_2302 0.40 -1.8 2,631,497 - PP_2302 0.40 +0.5 2,631,533 - PP_2302 0.42 -0.7 2,631,715 + PP_2302 0.49 -0.8 2,631,715 + PP_2302 0.49 -2.7 2,631,715 + PP_2302 0.49 -0.5 2,631,715 + PP_2302 0.49 -2.5 2,631,716 - PP_2302 0.49 -2.7 2,631,716 - PP_2302 0.49 -2.9 2,631,716 - PP_2302 0.49 -3.4 2,631,904 + PP_2302 0.57 -1.6 2,632,036 + PP_2302 0.63 -2.6 2,632,036 + PP_2302 0.63 -3.4 2,632,037 - PP_2302 0.63 -1.4 2,632,037 - PP_2302 0.63 +0.4 2,632,123 + PP_2302 0.66 +0.0 2,632,124 - PP_2302 0.66 -1.0 2,632,127 - PP_2302 0.66 -2.1 2,632,127 - PP_2302 0.66 +0.9 2,632,200 + PP_2302 0.69 -0.6 2,632,201 - PP_2302 0.69 -2.8 2,632,908 + +1.0 2,632,930 + -4.3 2,632,930 + -0.4 2,632,930 + -0.0 2,632,931 - -0.8 2,632,981 + -0.4 2,633,040 + -0.5 2,633,044 + +1.2 2,633,044 + -1.4 2,633,048 + -1.3 2,633,048 + +0.1 2,633,048 + -1.9 2,633,355 + +0.0 2,633,355 + +0.1 2,633,355 + +2.5 2,633,356 - -1.4 2,633,356 - +0.9 2,633,440 + +0.3 2,633,517 + -0.4 2,633,518 - +0.6 2,633,655 + -1.9 2,633,655 + -3.2 2,633,655 + -2.2 2,633,655 + -2.9
Or see this region's nucleotide sequence