Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1772 and PP_1773 are separated by 299 nucleotides PP_1773 and PP_1774 overlap by 32 nucleotides PP_1774 and PP_1775 are separated by 102 nucleotides
PP_1772: PP_1772 - 30S ribosomal protein S1, at 1,979,331 to 1,981,061
_1772
PP_1773: PP_1773 - Integration host factor subunit beta, at 1,981,361 to 1,981,663
_1773
PP_1774: PP_1774 - conserved exported protein of unknown function, at 1,981,632 to 1,981,946
_1774
PP_1775: PP_1775 - Metallo-beta-lactamase family protein, at 1,982,049 to 1,983,482
_1775
Position (kb)
1981
1982 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1981.183 kb on - strand at 1981.221 kb on - strand at 1981.221 kb on - strand at 1981.224 kb on + strand at 1981.225 kb on - strand at 1981.225 kb on - strand at 1981.231 kb on + strand at 1981.271 kb on + strand at 1981.272 kb on - strand at 1981.360 kb on + strand at 1981.387 kb on + strand at 1981.387 kb on + strand at 1981.387 kb on + strand at 1981.388 kb on - strand at 1981.388 kb on - strand at 1981.726 kb on + strand, within PP_1774 at 1981.727 kb on - strand, within PP_1774 at 1981.727 kb on - strand, within PP_1774 at 1981.945 kb on - strand at 1981.945 kb on - strand at 1981.963 kb on + strand at 1981.963 kb on + strand at 1981.964 kb on - strand at 1981.964 kb on - strand at 1981.964 kb on - strand at 1981.964 kb on - strand at 1981.966 kb on + strand at 1981.967 kb on - strand at 1981.982 kb on + strand at 1981.982 kb on + strand at 1981.983 kb on - strand at 1981.983 kb on - strand at 1981.983 kb on - strand at 1981.983 kb on - strand at 1982.007 kb on + strand at 1982.008 kb on - strand at 1982.008 kb on - strand at 1982.008 kb on - strand at 1982.010 kb on + strand at 1982.011 kb on - strand at 1982.011 kb on - strand at 1982.052 kb on + strand at 1982.304 kb on - strand, within PP_1775 at 1982.359 kb on - strand, within PP_1775 at 1982.359 kb on - strand, within PP_1775 at 1982.401 kb on - strand, within PP_1775 at 1982.423 kb on + strand, within PP_1775 at 1982.424 kb on - strand, within PP_1775 at 1982.487 kb on - strand, within PP_1775 at 1982.526 kb on + strand, within PP_1775 at 1982.527 kb on - strand, within PP_1775 at 1982.527 kb on - strand, within PP_1775 at 1982.540 kb on + strand, within PP_1775 at 1982.569 kb on - strand, within PP_1775 at 1982.569 kb on - strand, within PP_1775 at 1982.664 kb on + strand, within PP_1775 at 1982.796 kb on + strand, within PP_1775
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 1,981,183 - +0.3 1,981,221 - +0.6 1,981,221 - -0.7 1,981,224 + -0.1 1,981,225 - +0.9 1,981,225 - +0.7 1,981,231 + +0.6 1,981,271 + +0.6 1,981,272 - -0.0 1,981,360 + -0.7 1,981,387 + -0.6 1,981,387 + -1.8 1,981,387 + -1.7 1,981,388 - -0.8 1,981,388 - +0.0 1,981,726 + PP_1774 0.30 +1.1 1,981,727 - PP_1774 0.30 +0.7 1,981,727 - PP_1774 0.30 +0.4 1,981,945 - +1.1 1,981,945 - +0.9 1,981,963 + -0.9 1,981,963 + -0.4 1,981,964 - +0.6 1,981,964 - +0.6 1,981,964 - -0.5 1,981,964 - +0.5 1,981,966 + -0.6 1,981,967 - +0.7 1,981,982 + +0.2 1,981,982 + +0.2 1,981,983 - -1.2 1,981,983 - +1.2 1,981,983 - +0.3 1,981,983 - +0.1 1,982,007 + +1.0 1,982,008 - +0.1 1,982,008 - +0.1 1,982,008 - +0.4 1,982,010 + +0.4 1,982,011 - +0.2 1,982,011 - +0.8 1,982,052 + +0.1 1,982,304 - PP_1775 0.18 +0.5 1,982,359 - PP_1775 0.22 -0.2 1,982,359 - PP_1775 0.22 -0.1 1,982,401 - PP_1775 0.25 +0.7 1,982,423 + PP_1775 0.26 +0.3 1,982,424 - PP_1775 0.26 +1.1 1,982,487 - PP_1775 0.31 +1.3 1,982,526 + PP_1775 0.33 +0.0 1,982,527 - PP_1775 0.33 +0.0 1,982,527 - PP_1775 0.33 -0.3 1,982,540 + PP_1775 0.34 -0.8 1,982,569 - PP_1775 0.36 +0.4 1,982,569 - PP_1775 0.36 +1.8 1,982,664 + PP_1775 0.43 +0.9 1,982,796 + PP_1775 0.52 -0.2
Or see this region's nucleotide sequence