Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0743 and PP_0744 are separated by 13 nucleotides PP_0744 and PP_0745 are separated by 35 nucleotides
PP_0743: PP_0743 - conserved protein with NAD(P)-binding Rossmann-fold domain, at 861,948 to 862,853
_0743
PP_0744: PP_0744 - Ferrochelatase, at 862,867 to 863,883
_0744
PP_0745: PP_0745 - putative uracil permease, at 863,919 to 865,184
_0745
Position (kb)
862
863
864 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 861.867 kb on - strand at 861.867 kb on - strand at 861.909 kb on + strand at 861.916 kb on - strand at 861.953 kb on + strand at 861.953 kb on + strand at 861.953 kb on + strand at 861.953 kb on + strand at 861.953 kb on + strand at 861.953 kb on + strand at 861.954 kb on - strand at 861.954 kb on - strand at 861.954 kb on - strand at 861.954 kb on - strand at 861.954 kb on - strand at 861.954 kb on - strand at 861.954 kb on - strand at 861.954 kb on - strand at 861.954 kb on - strand at 861.954 kb on - strand at 861.954 kb on - strand at 861.954 kb on - strand at 861.954 kb on - strand at 861.955 kb on + strand at 861.955 kb on + strand at 861.955 kb on + strand at 861.955 kb on + strand at 861.955 kb on + strand at 861.955 kb on + strand at 861.955 kb on + strand at 861.955 kb on + strand at 861.955 kb on + strand at 861.956 kb on - strand at 861.956 kb on - strand at 861.956 kb on - strand at 861.956 kb on - strand at 861.956 kb on - strand at 862.108 kb on + strand, within PP_0743 at 862.108 kb on + strand, within PP_0743 at 862.109 kb on - strand, within PP_0743 at 862.109 kb on - strand at 862.109 kb on - strand, within PP_0743 at 862.300 kb on - strand, within PP_0743 at 862.373 kb on + strand, within PP_0743 at 862.374 kb on - strand, within PP_0743 at 862.417 kb on + strand, within PP_0743 at 862.417 kb on + strand, within PP_0743 at 862.418 kb on - strand, within PP_0743 at 862.426 kb on + strand, within PP_0743 at 862.427 kb on - strand, within PP_0743 at 862.427 kb on - strand, within PP_0743 at 862.550 kb on + strand, within PP_0743 at 862.551 kb on - strand, within PP_0743 at 862.551 kb on - strand, within PP_0743 at 862.552 kb on + strand, within PP_0743 at 862.553 kb on - strand, within PP_0743 at 862.553 kb on - strand, within PP_0743 at 862.611 kb on + strand, within PP_0743 at 862.612 kb on - strand, within PP_0743 at 862.612 kb on - strand, within PP_0743 at 862.612 kb on - strand, within PP_0743 at 862.612 kb on - strand, within PP_0743 at 862.612 kb on - strand, within PP_0743 at 862.613 kb on + strand, within PP_0743 at 862.613 kb on + strand, within PP_0743 at 863.865 kb on + strand at 863.865 kb on + strand at 863.865 kb on + strand at 863.865 kb on + strand at 863.922 kb on + strand at 863.922 kb on + strand at 863.922 kb on + strand at 863.922 kb on + strand at 863.923 kb on - strand at 863.923 kb on - strand at 863.923 kb on - strand at 863.923 kb on - strand at 863.923 kb on - strand at 863.923 kb on - strand at 863.982 kb on - strand at 864.248 kb on + strand, within PP_0745 at 864.248 kb on + strand, within PP_0745 at 864.249 kb on - strand, within PP_0745 at 864.289 kb on + strand, within PP_0745 at 864.290 kb on - strand, within PP_0745 at 864.290 kb on - strand, within PP_0745 at 864.290 kb on - strand, within PP_0745 at 864.328 kb on + strand, within PP_0745 at 864.328 kb on + strand at 864.328 kb on + strand, within PP_0745 at 864.329 kb on - strand, within PP_0745 at 864.368 kb on + strand, within PP_0745 at 864.422 kb on - strand, within PP_0745 at 864.422 kb on - strand, within PP_0745 at 864.728 kb on - strand, within PP_0745
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 861,867 - -1.1 861,867 - +0.2 861,909 + -0.0 861,916 - +0.6 861,953 + -0.7 861,953 + -0.1 861,953 + -0.2 861,953 + +0.3 861,953 + +0.3 861,953 + -1.4 861,954 - -0.9 861,954 - -0.1 861,954 - +0.2 861,954 - +0.7 861,954 - +0.5 861,954 - +0.3 861,954 - +0.4 861,954 - -0.2 861,954 - -0.2 861,954 - -0.3 861,954 - +0.4 861,954 - +2.7 861,954 - -0.2 861,955 + +0.4 861,955 + +1.7 861,955 + +0.7 861,955 + +0.1 861,955 + -0.5 861,955 + +0.1 861,955 + -0.5 861,955 + +0.5 861,955 + +1.4 861,956 - -0.3 861,956 - -0.1 861,956 - +0.3 861,956 - +1.2 861,956 - -1.0 862,108 + PP_0743 0.18 -0.4 862,108 + PP_0743 0.18 +0.5 862,109 - PP_0743 0.18 -0.4 862,109 - +0.7 862,109 - PP_0743 0.18 -0.0 862,300 - PP_0743 0.39 -0.6 862,373 + PP_0743 0.47 -1.4 862,374 - PP_0743 0.47 -0.6 862,417 + PP_0743 0.52 +0.3 862,417 + PP_0743 0.52 +0.4 862,418 - PP_0743 0.52 +0.5 862,426 + PP_0743 0.53 -0.7 862,427 - PP_0743 0.53 -1.9 862,427 - PP_0743 0.53 -0.7 862,550 + PP_0743 0.66 -0.2 862,551 - PP_0743 0.67 -0.4 862,551 - PP_0743 0.67 -0.9 862,552 + PP_0743 0.67 -1.5 862,553 - PP_0743 0.67 -0.2 862,553 - PP_0743 0.67 -0.5 862,611 + PP_0743 0.73 +0.0 862,612 - PP_0743 0.73 +0.2 862,612 - PP_0743 0.73 -0.4 862,612 - PP_0743 0.73 -0.6 862,612 - PP_0743 0.73 +0.5 862,612 - PP_0743 0.73 -1.3 862,613 + PP_0743 0.73 +0.4 862,613 + PP_0743 0.73 +0.1 863,865 + +0.7 863,865 + -0.6 863,865 + -0.5 863,865 + -0.7 863,922 + -0.8 863,922 + -0.6 863,922 + +0.7 863,922 + +0.0 863,923 - -1.3 863,923 - -0.7 863,923 - +0.3 863,923 - +0.1 863,923 - +0.1 863,923 - -0.2 863,982 - +0.4 864,248 + PP_0745 0.26 +0.0 864,248 + PP_0745 0.26 -1.1 864,249 - PP_0745 0.26 -0.6 864,289 + PP_0745 0.29 +1.0 864,290 - PP_0745 0.29 -0.9 864,290 - PP_0745 0.29 +0.2 864,290 - PP_0745 0.29 -0.6 864,328 + PP_0745 0.32 -0.1 864,328 + +0.1 864,328 + PP_0745 0.32 -0.1 864,329 - PP_0745 0.32 +0.3 864,368 + PP_0745 0.35 -1.1 864,422 - PP_0745 0.40 -2.9 864,422 - PP_0745 0.40 -1.6 864,728 - PP_0745 0.64 +0.7
Or see this region's nucleotide sequence