Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0350 and PP_0351 are separated by 251 nucleotides PP_0351 and PP_0352 overlap by 4 nucleotides PP_0352 and PP_0353 are separated by 147 nucleotides
PP_0350: PP_0350 - Outer membrane ferrichrome-iron receptor, at 425,366 to 427,798
_0350
PP_0351: PP_0351 - putative Transmembrane sensor protein, at 428,050 to 429,006
_0351
PP_0352: PP_0352 - RNA polymerase sigma-70 factor, ECF subfamily, at 429,003 to 429,521
_0352
PP_0353: PP_0353 - Exonuclease, at 429,669 to 430,379
_0353
Position (kb) 428
429
430 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 427.134 kb on + strand, within PP_0350 at 427.134 kb on + strand, within PP_0350 at 427.134 kb on + strand, within PP_0350 at 427.134 kb on + strand, within PP_0350 at 427.134 kb on + strand, within PP_0350 at 427.134 kb on + strand, within PP_0350 at 427.135 kb on - strand, within PP_0350 at 427.213 kb on + strand, within PP_0350 at 427.249 kb on + strand, within PP_0350 at 427.251 kb on + strand, within PP_0350 at 427.251 kb on + strand, within PP_0350 at 427.251 kb on + strand, within PP_0350 at 427.251 kb on + strand, within PP_0350 at 427.252 kb on - strand, within PP_0350 at 427.252 kb on - strand, within PP_0350 at 427.383 kb on + strand, within PP_0350 at 427.383 kb on + strand, within PP_0350 at 427.384 kb on - strand, within PP_0350 at 427.482 kb on + strand, within PP_0350 at 427.482 kb on + strand, within PP_0350 at 427.482 kb on + strand, within PP_0350 at 427.483 kb on - strand, within PP_0350 at 427.558 kb on - strand at 427.558 kb on - strand at 427.680 kb on + strand at 427.680 kb on + strand at 427.681 kb on - strand at 427.777 kb on + strand at 427.777 kb on + strand at 427.777 kb on + strand at 427.778 kb on - strand at 427.778 kb on - strand at 427.973 kb on + strand at 427.973 kb on + strand at 427.996 kb on - strand at 428.051 kb on + strand at 428.051 kb on + strand at 428.082 kb on + strand at 428.082 kb on + strand at 428.083 kb on - strand at 428.083 kb on - strand at 428.248 kb on + strand, within PP_0351 at 428.248 kb on + strand, within PP_0351 at 428.248 kb on + strand, within PP_0351 at 428.249 kb on - strand, within PP_0351 at 428.249 kb on - strand, within PP_0351 at 428.249 kb on - strand, within PP_0351 at 428.425 kb on - strand, within PP_0351 at 428.492 kb on - strand, within PP_0351 at 428.577 kb on - strand, within PP_0351 at 428.645 kb on - strand, within PP_0351 at 428.645 kb on - strand, within PP_0351 at 428.645 kb on - strand, within PP_0351 at 428.682 kb on - strand, within PP_0351 at 428.682 kb on - strand, within PP_0351 at 429.031 kb on + strand at 429.031 kb on + strand at 429.031 kb on + strand at 429.032 kb on - strand at 429.032 kb on - strand at 429.032 kb on - strand at 429.058 kb on + strand, within PP_0352 at 429.059 kb on - strand, within PP_0352 at 429.268 kb on + strand, within PP_0352 at 429.268 kb on + strand, within PP_0352 at 429.268 kb on + strand, within PP_0352 at 429.476 kb on - strand at 429.641 kb on + strand at 429.642 kb on - strand at 429.664 kb on + strand at 429.665 kb on - strand at 429.670 kb on + strand at 429.670 kb on + strand at 429.671 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 427,134 + PP_0350 0.73 -0.3 427,134 + PP_0350 0.73 -0.1 427,134 + PP_0350 0.73 -1.5 427,134 + PP_0350 0.73 -0.6 427,134 + PP_0350 0.73 +0.3 427,134 + PP_0350 0.73 -0.3 427,135 - PP_0350 0.73 +0.1 427,213 + PP_0350 0.76 +0.7 427,249 + PP_0350 0.77 -1.2 427,251 + PP_0350 0.77 -0.4 427,251 + PP_0350 0.77 +0.2 427,251 + PP_0350 0.77 +0.7 427,251 + PP_0350 0.77 +0.2 427,252 - PP_0350 0.78 -0.3 427,252 - PP_0350 0.78 -2.9 427,383 + PP_0350 0.83 +0.0 427,383 + PP_0350 0.83 +0.0 427,384 - PP_0350 0.83 -0.1 427,482 + PP_0350 0.87 +0.4 427,482 + PP_0350 0.87 -0.2 427,482 + PP_0350 0.87 -0.3 427,483 - PP_0350 0.87 -0.8 427,558 - +0.5 427,558 - +0.7 427,680 + -0.1 427,680 + -0.7 427,681 - -2.0 427,777 + -0.1 427,777 + -0.1 427,777 + -0.3 427,778 - +1.1 427,778 - +0.4 427,973 + -0.6 427,973 + -0.3 427,996 - -1.3 428,051 + +0.3 428,051 + -0.4 428,082 + +0.7 428,082 + +0.2 428,083 - -0.6 428,083 - -0.5 428,248 + PP_0351 0.21 +0.6 428,248 + PP_0351 0.21 -1.2 428,248 + PP_0351 0.21 -2.3 428,249 - PP_0351 0.21 -0.2 428,249 - PP_0351 0.21 -0.9 428,249 - PP_0351 0.21 -3.2 428,425 - PP_0351 0.39 -0.5 428,492 - PP_0351 0.46 -3.3 428,577 - PP_0351 0.55 -1.7 428,645 - PP_0351 0.62 -0.5 428,645 - PP_0351 0.62 -0.9 428,645 - PP_0351 0.62 -0.9 428,682 - PP_0351 0.66 -0.9 428,682 - PP_0351 0.66 -0.1 429,031 + -2.5 429,031 + -0.8 429,031 + -0.1 429,032 - +0.7 429,032 - -2.1 429,032 - -1.5 429,058 + PP_0352 0.11 -0.3 429,059 - PP_0352 0.11 -0.4 429,268 + PP_0352 0.51 -0.9 429,268 + PP_0352 0.51 -1.2 429,268 + PP_0352 0.51 +0.0 429,476 - -3.6 429,641 + -0.6 429,642 - -1.7 429,664 + +0.4 429,665 - -1.1 429,670 + +0.5 429,670 + +0.8 429,671 - +1.6
Or see this region's nucleotide sequence