Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0239 and PP_0240 overlap by 4 nucleotides PP_0240 and PP_0241 are separated by 27 nucleotides PP_0241 and PP_0242 are separated by 141 nucleotides PP_0242 and PP_0243 overlap by 1 nucleotides
PP_0239: PP_0239 - aliphatic sulfonate ABC transporter - permease subunit / transport of isethionate, at 293,803 to 294,600
_0239
PP_0240: PP_0240 - Aliphatic sulfonates import ATP-binding protein SsuB, at 294,597 to 295,409
_0240
PP_0241: PP_0241 - Transporter, at 295,437 to 295,652
_0241
PP_0242: PP_0242 - Transcriptional regulator, TetR family, at 295,794 to 296,426
_0242
PP_0243: PP_0243 - Glutamate--cysteine ligase, at 296,426 to 298,003
_0243
Position (kb) 295
296 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 294.476 kb on + strand, within PP_0239 at 294.496 kb on + strand, within PP_0239 at 294.496 kb on + strand, within PP_0239 at 294.497 kb on - strand, within PP_0239 at 294.572 kb on - strand at 294.572 kb on - strand at 295.168 kb on + strand, within PP_0240 at 295.168 kb on + strand, within PP_0240 at 295.169 kb on - strand, within PP_0240 at 295.169 kb on - strand, within PP_0240 at 295.372 kb on + strand at 295.373 kb on - strand at 295.442 kb on + strand at 295.443 kb on - strand at 295.443 kb on - strand at 295.443 kb on - strand at 295.478 kb on + strand, within PP_0241 at 295.479 kb on - strand, within PP_0241 at 295.559 kb on + strand, within PP_0241 at 295.560 kb on - strand, within PP_0241 at 295.637 kb on + strand at 295.637 kb on + strand at 295.828 kb on + strand at 295.829 kb on - strand at 295.829 kb on - strand at 295.840 kb on + strand at 295.841 kb on - strand at 295.841 kb on - strand at 295.915 kb on + strand, within PP_0242 at 295.915 kb on + strand, within PP_0242 at 295.915 kb on + strand, within PP_0242 at 295.915 kb on + strand, within PP_0242 at 295.915 kb on + strand, within PP_0242 at 295.916 kb on - strand, within PP_0242 at 295.916 kb on - strand, within PP_0242 at 295.916 kb on - strand, within PP_0242 at 295.916 kb on - strand, within PP_0242 at 295.916 kb on - strand, within PP_0242 at 295.916 kb on - strand, within PP_0242 at 296.053 kb on - strand, within PP_0242 at 296.110 kb on + strand, within PP_0242 at 296.110 kb on + strand, within PP_0242 at 296.111 kb on - strand, within PP_0242 at 296.111 kb on - strand, within PP_0242 at 296.114 kb on + strand, within PP_0242 at 296.115 kb on - strand, within PP_0242 at 296.115 kb on - strand, within PP_0242 at 296.225 kb on + strand, within PP_0242 at 296.225 kb on + strand, within PP_0242 at 296.226 kb on - strand, within PP_0242 at 296.226 kb on - strand, within PP_0242 at 296.308 kb on + strand, within PP_0242 at 296.308 kb on + strand, within PP_0242 at 296.312 kb on + strand, within PP_0242 at 296.458 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 294,476 + PP_0239 0.84 -0.1 294,496 + PP_0239 0.87 -0.8 294,496 + PP_0239 0.87 +0.1 294,497 - PP_0239 0.87 +0.1 294,572 - +0.1 294,572 - +0.4 295,168 + PP_0240 0.70 +0.5 295,168 + PP_0240 0.70 -0.2 295,169 - PP_0240 0.70 -1.0 295,169 - PP_0240 0.70 +0.8 295,372 + +1.1 295,373 - +0.2 295,442 + -1.5 295,443 - -0.1 295,443 - +1.3 295,443 - +0.6 295,478 + PP_0241 0.19 +0.3 295,479 - PP_0241 0.19 +1.0 295,559 + PP_0241 0.56 +0.5 295,560 - PP_0241 0.57 +0.2 295,637 + +0.2 295,637 + -0.3 295,828 + +0.8 295,829 - -1.1 295,829 - +0.1 295,840 + -1.1 295,841 - +0.3 295,841 - -0.2 295,915 + PP_0242 0.19 -0.9 295,915 + PP_0242 0.19 -0.2 295,915 + PP_0242 0.19 -0.7 295,915 + PP_0242 0.19 -0.8 295,915 + PP_0242 0.19 +0.2 295,916 - PP_0242 0.19 -0.7 295,916 - PP_0242 0.19 +0.0 295,916 - PP_0242 0.19 +0.2 295,916 - PP_0242 0.19 +0.6 295,916 - PP_0242 0.19 +0.2 295,916 - PP_0242 0.19 -0.5 296,053 - PP_0242 0.41 -0.9 296,110 + PP_0242 0.50 +0.3 296,110 + PP_0242 0.50 -1.0 296,111 - PP_0242 0.50 +0.3 296,111 - PP_0242 0.50 -2.2 296,114 + PP_0242 0.51 +0.8 296,115 - PP_0242 0.51 -0.6 296,115 - PP_0242 0.51 -0.9 296,225 + PP_0242 0.68 +0.1 296,225 + PP_0242 0.68 +0.2 296,226 - PP_0242 0.68 +1.6 296,226 - PP_0242 0.68 +0.2 296,308 + PP_0242 0.81 +0.0 296,308 + PP_0242 0.81 -2.8 296,312 + PP_0242 0.82 -0.4 296,458 - +0.1
Or see this region's nucleotide sequence