Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5131 and PP_5132 are separated by 131 nucleotides PP_5132 and PP_5133 are separated by 54 nucleotides PP_5133 and PP_5134 overlap by 14 nucleotides
PP_5131: PP_5131 - sodium-cystine symporter, at 5,853,981 to 5,855,372
_5131
PP_5132: PP_5132 - dihydrofolate reductase, at 5,855,504 to 5,856,019
_5132
PP_5133: PP_5133 - conserved membrane protein of unknown function, at 5,856,074 to 5,857,465
_5133
PP_5134: PP_5134 - conserved protein of unknown function, at 5,857,452 to 5,858,822
_5134
Position (kb) 5856
5857
5858 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5855.457 kb on - strand at 5856.014 kb on - strand at 5856.014 kb on - strand at 5856.014 kb on - strand at 5856.014 kb on - strand at 5856.032 kb on - strand at 5856.199 kb on - strand at 5856.199 kb on - strand at 5856.199 kb on - strand at 5856.435 kb on + strand, within PP_5133 at 5856.435 kb on + strand, within PP_5133 at 5856.435 kb on + strand, within PP_5133 at 5856.436 kb on - strand, within PP_5133 at 5856.436 kb on - strand, within PP_5133 at 5856.508 kb on - strand, within PP_5133 at 5856.612 kb on + strand, within PP_5133 at 5856.657 kb on + strand, within PP_5133 at 5856.657 kb on + strand, within PP_5133 at 5856.657 kb on + strand, within PP_5133 at 5856.658 kb on - strand, within PP_5133 at 5856.658 kb on - strand, within PP_5133 at 5856.881 kb on + strand, within PP_5133 at 5856.882 kb on - strand, within PP_5133 at 5856.882 kb on - strand, within PP_5133 at 5856.882 kb on - strand, within PP_5133 at 5856.978 kb on - strand, within PP_5133 at 5857.327 kb on + strand at 5857.493 kb on + strand at 5857.493 kb on + strand at 5857.493 kb on + strand at 5857.494 kb on - strand at 5857.494 kb on - strand at 5857.517 kb on - strand at 5857.574 kb on + strand at 5857.574 kb on + strand at 5857.574 kb on + strand at 5857.575 kb on - strand at 5857.633 kb on - strand, within PP_5134 at 5857.633 kb on - strand, within PP_5134 at 5857.783 kb on + strand, within PP_5134 at 5857.783 kb on + strand, within PP_5134 at 5857.783 kb on + strand, within PP_5134 at 5857.784 kb on - strand, within PP_5134 at 5857.784 kb on - strand, within PP_5134 at 5857.818 kb on + strand, within PP_5134 at 5857.818 kb on + strand, within PP_5134 at 5857.819 kb on - strand, within PP_5134 at 5857.987 kb on + strand, within PP_5134 at 5857.987 kb on + strand, within PP_5134 at 5858.035 kb on - strand, within PP_5134 at 5858.239 kb on + strand, within PP_5134 at 5858.239 kb on + strand, within PP_5134 at 5858.239 kb on + strand, within PP_5134 at 5858.240 kb on - strand, within PP_5134 at 5858.240 kb on - strand, within PP_5134 at 5858.240 kb on - strand, within PP_5134 at 5858.283 kb on + strand, within PP_5134 at 5858.284 kb on - strand, within PP_5134 at 5858.457 kb on - strand, within PP_5134
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 5,855,457 - +0.6 5,856,014 - +0.7 5,856,014 - -1.0 5,856,014 - +1.3 5,856,014 - -0.5 5,856,032 - -1.1 5,856,199 - +0.4 5,856,199 - +0.1 5,856,199 - -1.2 5,856,435 + PP_5133 0.26 -0.2 5,856,435 + PP_5133 0.26 -1.1 5,856,435 + PP_5133 0.26 +1.7 5,856,436 - PP_5133 0.26 -1.0 5,856,436 - PP_5133 0.26 +1.4 5,856,508 - PP_5133 0.31 -0.2 5,856,612 + PP_5133 0.39 -1.2 5,856,657 + PP_5133 0.42 +2.5 5,856,657 + PP_5133 0.42 +1.7 5,856,657 + PP_5133 0.42 +1.6 5,856,658 - PP_5133 0.42 +1.9 5,856,658 - PP_5133 0.42 -2.0 5,856,881 + PP_5133 0.58 +0.4 5,856,882 - PP_5133 0.58 +2.0 5,856,882 - PP_5133 0.58 -3.5 5,856,882 - PP_5133 0.58 -0.1 5,856,978 - PP_5133 0.65 -0.7 5,857,327 + +0.4 5,857,493 + -0.4 5,857,493 + +0.9 5,857,493 + +2.0 5,857,494 - -3.9 5,857,494 - -0.8 5,857,517 - +1.2 5,857,574 + +0.6 5,857,574 + -0.2 5,857,574 + -1.1 5,857,575 - -1.4 5,857,633 - PP_5134 0.13 +0.5 5,857,633 - PP_5134 0.13 -0.2 5,857,783 + PP_5134 0.24 +0.0 5,857,783 + PP_5134 0.24 -0.8 5,857,783 + PP_5134 0.24 -0.2 5,857,784 - PP_5134 0.24 -0.3 5,857,784 - PP_5134 0.24 -0.4 5,857,818 + PP_5134 0.27 +0.4 5,857,818 + PP_5134 0.27 +0.0 5,857,819 - PP_5134 0.27 -0.5 5,857,987 + PP_5134 0.39 -0.5 5,857,987 + PP_5134 0.39 +0.8 5,858,035 - PP_5134 0.43 +1.2 5,858,239 + PP_5134 0.57 -0.7 5,858,239 + PP_5134 0.57 +0.5 5,858,239 + PP_5134 0.57 +1.2 5,858,240 - PP_5134 0.57 -1.4 5,858,240 - PP_5134 0.57 +0.9 5,858,240 - PP_5134 0.57 -0.4 5,858,283 + PP_5134 0.61 +1.4 5,858,284 - PP_5134 0.61 -1.0 5,858,457 - PP_5134 0.73 +0.2
Or see this region's nucleotide sequence