Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2139 and PP_2140 are separated by 12 nucleotides PP_2140 and PP_2141 are separated by 50 nucleotides PP_2141 and PP_2142 are separated by 50 nucleotides PP_2142 and PP_2143 are separated by 13 nucleotides
PP_2139: PP_2139 - DNA topoisomerase I, at 2,440,863 to 2,443,472
_2139
PP_2140: PP_2140 - conserved protein of unknown function, at 2,443,485 to 2,444,078
_2140
PP_2141: PP_2141 - conserved protein of unknown function, at 2,444,129 to 2,444,362
_2141
PP_2142: PP_2142 - Cell division inhibitor SulA, at 2,444,413 to 2,444,889
_2142
PP_2143: PP_2143 - transcriptional repressor, at 2,444,903 to 2,445,511
_2143
Position (kb)
2443
2444
2445 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2443.179 kb on + strand, within PP_2139 at 2443.179 kb on + strand, within PP_2139 at 2443.179 kb on + strand, within PP_2139 at 2443.180 kb on - strand at 2443.386 kb on + strand at 2443.386 kb on + strand at 2443.386 kb on + strand at 2443.387 kb on - strand at 2443.434 kb on + strand at 2443.464 kb on - strand at 2443.518 kb on + strand at 2443.529 kb on + strand at 2443.529 kb on + strand at 2443.540 kb on + strand at 2443.540 kb on + strand at 2443.540 kb on + strand at 2443.541 kb on - strand at 2443.578 kb on + strand, within PP_2140 at 2443.710 kb on + strand, within PP_2140 at 2443.710 kb on + strand, within PP_2140 at 2443.711 kb on - strand, within PP_2140 at 2443.711 kb on - strand, within PP_2140 at 2443.711 kb on - strand, within PP_2140 at 2443.752 kb on + strand, within PP_2140 at 2443.811 kb on + strand, within PP_2140 at 2443.891 kb on - strand, within PP_2140 at 2443.891 kb on - strand, within PP_2140 at 2444.034 kb on - strand at 2444.096 kb on - strand at 2444.101 kb on - strand at 2444.128 kb on + strand at 2444.128 kb on + strand at 2444.128 kb on + strand at 2444.128 kb on + strand at 2444.128 kb on + strand at 2444.129 kb on - strand at 2444.129 kb on - strand at 2444.129 kb on - strand at 2444.129 kb on - strand at 2444.129 kb on - strand at 2444.135 kb on - strand at 2444.377 kb on - strand at 2444.465 kb on - strand, within PP_2142 at 2444.595 kb on + strand, within PP_2142 at 2444.595 kb on + strand, within PP_2142 at 2444.596 kb on - strand, within PP_2142
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 2,443,179 + PP_2139 0.89 -2.6 2,443,179 + PP_2139 0.89 -3.0 2,443,179 + PP_2139 0.89 -2.8 2,443,180 - -1.0 2,443,386 + -0.8 2,443,386 + -2.5 2,443,386 + -2.4 2,443,387 - -1.2 2,443,434 + -1.3 2,443,464 - -2.9 2,443,518 + +0.9 2,443,529 + +0.1 2,443,529 + +0.9 2,443,540 + +0.8 2,443,540 + -0.9 2,443,540 + +0.6 2,443,541 - +0.1 2,443,578 + PP_2140 0.16 -4.1 2,443,710 + PP_2140 0.38 -0.3 2,443,710 + PP_2140 0.38 -0.7 2,443,711 - PP_2140 0.38 -2.5 2,443,711 - PP_2140 0.38 -1.1 2,443,711 - PP_2140 0.38 -3.0 2,443,752 + PP_2140 0.45 -1.6 2,443,811 + PP_2140 0.55 +0.7 2,443,891 - PP_2140 0.68 -3.3 2,443,891 - PP_2140 0.68 -0.3 2,444,034 - -0.4 2,444,096 - +0.5 2,444,101 - -0.2 2,444,128 + -1.2 2,444,128 + -0.4 2,444,128 + -0.2 2,444,128 + +0.8 2,444,128 + +1.2 2,444,129 - +0.4 2,444,129 - +0.2 2,444,129 - +0.4 2,444,129 - -3.6 2,444,129 - +0.8 2,444,135 - -0.3 2,444,377 - -1.4 2,444,465 - PP_2142 0.11 +1.2 2,444,595 + PP_2142 0.38 +0.2 2,444,595 + PP_2142 0.38 +0.2 2,444,596 - PP_2142 0.38 +0.2
Or see this region's nucleotide sequence