Experiment: R2A with Fusaric 480 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0774 and PP_0775 are separated by 31 nucleotides PP_0775 and PP_0776 overlap by 2 nucleotides PP_0776 and PP_0777 are separated by 145 nucleotides PP_0777 and PP_0778 are separated by 63 nucleotides
PP_0774: PP_0774 - Phosphate acetyltransferase, at 891,627 to 893,714
_0774
PP_0775: PP_0775 - conserved protein of unknown function, at 893,746 to 894,048
_0775
PP_0776: PP_0776 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 894,047 to 894,532
_0776
PP_0777: PP_0777 - Glutathione peroxidase, at 894,678 to 895,160
_0777
PP_0778: PP_0778 - Glycosyl transferase, group 1 family protein, at 895,224 to 896,414
_0778
Position (kb) 894
895
896 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 893.731 kb on - strand at 893.731 kb on - strand at 893.761 kb on - strand at 894.004 kb on + strand, within PP_0775 at 894.004 kb on + strand, within PP_0775 at 894.005 kb on - strand, within PP_0775 at 894.019 kb on + strand at 894.083 kb on + strand at 894.083 kb on + strand at 894.084 kb on - strand at 894.084 kb on - strand at 894.084 kb on - strand at 894.085 kb on + strand at 894.086 kb on - strand at 894.166 kb on + strand, within PP_0776 at 894.248 kb on + strand, within PP_0776 at 894.257 kb on + strand, within PP_0776 at 894.544 kb on - strand at 894.544 kb on - strand at 894.624 kb on + strand at 894.624 kb on + strand at 894.801 kb on + strand, within PP_0777 at 894.823 kb on - strand, within PP_0777 at 894.831 kb on + strand, within PP_0777 at 894.832 kb on - strand, within PP_0777 at 894.832 kb on - strand, within PP_0777 at 894.832 kb on - strand, within PP_0777 at 894.934 kb on - strand, within PP_0777 at 894.934 kb on - strand, within PP_0777 at 894.997 kb on - strand, within PP_0777 at 894.997 kb on - strand, within PP_0777 at 895.040 kb on + strand, within PP_0777 at 895.040 kb on + strand, within PP_0777 at 895.040 kb on + strand, within PP_0777 at 895.041 kb on - strand, within PP_0777 at 895.041 kb on - strand, within PP_0777 at 895.263 kb on - strand at 895.295 kb on - strand at 895.467 kb on - strand, within PP_0778 at 895.467 kb on - strand, within PP_0778 at 895.492 kb on + strand, within PP_0778 at 895.492 kb on + strand, within PP_0778 at 895.493 kb on - strand, within PP_0778 at 895.501 kb on - strand, within PP_0778 at 895.588 kb on + strand, within PP_0778 at 895.588 kb on + strand, within PP_0778 at 895.589 kb on - strand, within PP_0778 at 895.589 kb on - strand, within PP_0778 at 895.711 kb on + strand, within PP_0778 at 895.722 kb on + strand, within PP_0778 at 895.981 kb on + strand, within PP_0778 at 895.981 kb on + strand, within PP_0778 at 895.981 kb on + strand, within PP_0778 at 895.982 kb on - strand, within PP_0778 at 895.982 kb on - strand, within PP_0778 at 896.017 kb on + strand, within PP_0778 at 896.018 kb on - strand, within PP_0778 at 896.018 kb on - strand, within PP_0778 at 896.029 kb on + strand, within PP_0778 at 896.029 kb on + strand, within PP_0778 at 896.128 kb on + strand, within PP_0778 at 896.128 kb on + strand, within PP_0778 at 896.128 kb on + strand, within PP_0778 at 896.129 kb on - strand, within PP_0778 at 896.129 kb on - strand, within PP_0778 at 896.129 kb on - strand, within PP_0778 at 896.129 kb on - strand, within PP_0778 at 896.134 kb on - strand, within PP_0778 at 896.134 kb on - strand, within PP_0778 at 896.134 kb on - strand, within PP_0778 at 896.134 kb on - strand, within PP_0778
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 480 ug/mL remove 893,731 - +1.3 893,731 - +0.9 893,761 - -0.6 894,004 + PP_0775 0.85 -0.9 894,004 + PP_0775 0.85 +0.9 894,005 - PP_0775 0.85 -0.7 894,019 + +0.3 894,083 + +1.3 894,083 + -2.4 894,084 - -2.5 894,084 - -0.4 894,084 - +0.9 894,085 + -1.4 894,086 - +1.9 894,166 + PP_0776 0.24 -2.2 894,248 + PP_0776 0.41 +1.7 894,257 + PP_0776 0.43 -2.7 894,544 - +0.7 894,544 - +0.6 894,624 + +0.6 894,624 + +0.6 894,801 + PP_0777 0.25 -1.0 894,823 - PP_0777 0.30 +0.2 894,831 + PP_0777 0.32 -1.1 894,832 - PP_0777 0.32 -2.7 894,832 - PP_0777 0.32 -0.2 894,832 - PP_0777 0.32 -0.2 894,934 - PP_0777 0.53 -1.7 894,934 - PP_0777 0.53 -2.2 894,997 - PP_0777 0.66 -0.0 894,997 - PP_0777 0.66 +0.9 895,040 + PP_0777 0.75 -2.6 895,040 + PP_0777 0.75 -0.9 895,040 + PP_0777 0.75 -1.1 895,041 - PP_0777 0.75 -0.6 895,041 - PP_0777 0.75 -0.8 895,263 - -0.2 895,295 - -0.3 895,467 - PP_0778 0.20 +0.1 895,467 - PP_0778 0.20 +0.9 895,492 + PP_0778 0.23 +1.7 895,492 + PP_0778 0.23 -2.4 895,493 - PP_0778 0.23 +0.8 895,501 - PP_0778 0.23 +0.2 895,588 + PP_0778 0.31 -0.4 895,588 + PP_0778 0.31 -0.2 895,589 - PP_0778 0.31 +0.5 895,589 - PP_0778 0.31 -0.8 895,711 + PP_0778 0.41 +0.8 895,722 + PP_0778 0.42 +1.6 895,981 + PP_0778 0.64 +0.4 895,981 + PP_0778 0.64 -0.8 895,981 + PP_0778 0.64 +0.4 895,982 - PP_0778 0.64 -0.3 895,982 - PP_0778 0.64 -0.9 896,017 + PP_0778 0.67 +0.7 896,018 - PP_0778 0.67 -3.7 896,018 - PP_0778 0.67 +0.2 896,029 + PP_0778 0.68 -1.1 896,029 + PP_0778 0.68 +0.6 896,128 + PP_0778 0.76 +0.0 896,128 + PP_0778 0.76 +0.6 896,128 + PP_0778 0.76 -0.0 896,129 - PP_0778 0.76 +0.8 896,129 - PP_0778 0.76 +0.6 896,129 - PP_0778 0.76 -1.4 896,129 - PP_0778 0.76 -0.6 896,134 - PP_0778 0.76 -0.6 896,134 - PP_0778 0.76 -1.3 896,134 - PP_0778 0.76 +1.0 896,134 - PP_0778 0.76 -2.7
Or see this region's nucleotide sequence