Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5407 and PP_5408 are separated by 593 nucleotides PP_5408 and PP_5409 are separated by 171 nucleotides
PP_5407: PP_5407 - Transposition protein, TnsD-related protein, at 6,166,921 to 6,168,519
_5407
PP_5408: PP_5408 - conserved protein of unknown function, at 6,169,113 to 6,170,294
_5408
PP_5409: PP_5409 - L-glutamine/D-fructose-6-phosphate aminotransferase, at 6,170,466 to 6,172,301
_5409
Position (kb)
6169
6170
6171 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6168.165 kb on + strand, within PP_5407 at 6168.241 kb on + strand, within PP_5407 at 6168.572 kb on - strand at 6168.813 kb on - strand at 6168.813 kb on - strand at 6168.813 kb on - strand at 6168.839 kb on + strand at 6168.839 kb on + strand at 6168.840 kb on - strand at 6168.875 kb on - strand at 6168.875 kb on - strand at 6168.875 kb on - strand at 6168.882 kb on + strand at 6168.882 kb on + strand at 6168.883 kb on - strand at 6168.901 kb on + strand at 6168.902 kb on - strand at 6168.959 kb on + strand at 6168.959 kb on + strand at 6168.959 kb on + strand at 6168.959 kb on + strand at 6168.959 kb on + strand at 6169.065 kb on + strand at 6169.065 kb on + strand at 6169.066 kb on - strand at 6169.066 kb on - strand at 6169.071 kb on + strand at 6169.083 kb on + strand at 6169.109 kb on + strand at 6169.109 kb on + strand at 6169.110 kb on - strand at 6169.110 kb on - strand at 6169.114 kb on + strand at 6169.114 kb on + strand at 6169.145 kb on + strand at 6169.146 kb on - strand at 6169.146 kb on - strand at 6169.236 kb on + strand, within PP_5408 at 6169.294 kb on + strand, within PP_5408 at 6169.295 kb on - strand, within PP_5408 at 6169.360 kb on - strand, within PP_5408 at 6169.465 kb on - strand, within PP_5408 at 6169.483 kb on - strand, within PP_5408 at 6169.508 kb on + strand, within PP_5408 at 6169.611 kb on - strand, within PP_5408 at 6169.727 kb on - strand, within PP_5408 at 6169.786 kb on - strand, within PP_5408 at 6169.816 kb on - strand, within PP_5408 at 6169.816 kb on - strand, within PP_5408 at 6169.868 kb on - strand, within PP_5408 at 6169.905 kb on + strand, within PP_5408 at 6169.906 kb on - strand, within PP_5408 at 6169.906 kb on - strand, within PP_5408 at 6169.906 kb on - strand, within PP_5408 at 6169.906 kb on - strand, within PP_5408 at 6169.906 kb on - strand, within PP_5408 at 6169.953 kb on - strand, within PP_5408 at 6169.978 kb on + strand, within PP_5408 at 6170.064 kb on - strand, within PP_5408 at 6170.211 kb on + strand at 6170.212 kb on - strand at 6170.254 kb on + strand at 6170.254 kb on + strand at 6170.407 kb on + strand at 6170.407 kb on + strand at 6170.408 kb on - strand at 6170.408 kb on - strand at 6170.428 kb on - strand at 6170.461 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 6,168,165 + PP_5407 0.78 +0.3 6,168,241 + PP_5407 0.83 +0.8 6,168,572 - -0.2 6,168,813 - +0.6 6,168,813 - +0.1 6,168,813 - +0.4 6,168,839 + -2.3 6,168,839 + -2.0 6,168,840 - +0.3 6,168,875 - +0.7 6,168,875 - -0.3 6,168,875 - +0.5 6,168,882 + +2.0 6,168,882 + +0.5 6,168,883 - +0.4 6,168,901 + +1.0 6,168,902 - -0.0 6,168,959 + +1.0 6,168,959 + -1.7 6,168,959 + -3.1 6,168,959 + +0.8 6,168,959 + +0.7 6,169,065 + +1.4 6,169,065 + +0.6 6,169,066 - -0.0 6,169,066 - +0.0 6,169,071 + -0.3 6,169,083 + +0.7 6,169,109 + +1.3 6,169,109 + +0.7 6,169,110 - -0.1 6,169,110 - +0.3 6,169,114 + -0.2 6,169,114 + -0.1 6,169,145 + +1.1 6,169,146 - +1.1 6,169,146 - +1.3 6,169,236 + PP_5408 0.10 +0.7 6,169,294 + PP_5408 0.15 +1.5 6,169,295 - PP_5408 0.15 -1.6 6,169,360 - PP_5408 0.21 -0.8 6,169,465 - PP_5408 0.30 +0.3 6,169,483 - PP_5408 0.31 -1.6 6,169,508 + PP_5408 0.33 +0.2 6,169,611 - PP_5408 0.42 -0.4 6,169,727 - PP_5408 0.52 +0.1 6,169,786 - PP_5408 0.57 -0.7 6,169,816 - PP_5408 0.59 -1.3 6,169,816 - PP_5408 0.59 -1.6 6,169,868 - PP_5408 0.64 +0.6 6,169,905 + PP_5408 0.67 -0.4 6,169,906 - PP_5408 0.67 -0.8 6,169,906 - PP_5408 0.67 -0.7 6,169,906 - PP_5408 0.67 -1.1 6,169,906 - PP_5408 0.67 -0.6 6,169,906 - PP_5408 0.67 -1.4 6,169,953 - PP_5408 0.71 -0.8 6,169,978 + PP_5408 0.73 -2.1 6,170,064 - PP_5408 0.80 -0.4 6,170,211 + +1.0 6,170,212 - +0.8 6,170,254 + +1.6 6,170,254 + -0.4 6,170,407 + -0.1 6,170,407 + -0.4 6,170,408 - -0.8 6,170,408 - +0.1 6,170,428 - +0.4 6,170,461 - -2.9
Or see this region's nucleotide sequence