Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2842 and PP_2843 are separated by 9 nucleotides PP_2843 and PP_2844 are separated by 19 nucleotides PP_2844 and PP_2845 overlap by 4 nucleotides PP_2845 and PP_2846 are separated by 9 nucleotides PP_2846 and PP_2847 are separated by 18 nucleotides
PP_2842: PP_2842 - urease accessory protein, at 3,246,902 to 3,247,735
_2842
PP_2843: PP_2843 - urease subunit gamma, at 3,247,745 to 3,248,047
_2843
PP_2844: PP_2844 - Urease subunit beta, at 3,248,067 to 3,248,384
_2844
PP_2845: PP_2845 - urease subunit alpha, at 3,248,381 to 3,250,084
_2845
PP_2846: PP_2846 - urease accessory protein, at 3,250,094 to 3,250,597
_2846
PP_2847: PP_2847 - Urease accessory protein UreJ, at 3,250,616 to 3,251,161
_2847
Position (kb)
3248
3249
3250
3251 Strain fitness (log2 ratio)
-2
-1
0
1 at 3247.500 kb on + strand, within PP_2842 at 3247.561 kb on - strand, within PP_2842 at 3247.729 kb on + strand at 3247.838 kb on + strand, within PP_2843 at 3248.193 kb on + strand, within PP_2844 at 3248.194 kb on - strand, within PP_2844 at 3248.194 kb on - strand, within PP_2844 at 3248.316 kb on + strand, within PP_2844 at 3248.337 kb on + strand, within PP_2844 at 3248.338 kb on - strand at 3248.338 kb on - strand, within PP_2844 at 3248.389 kb on + strand at 3248.495 kb on + strand at 3248.496 kb on - strand at 3249.052 kb on + strand, within PP_2845 at 3249.053 kb on - strand, within PP_2845 at 3249.196 kb on + strand, within PP_2845 at 3249.291 kb on - strand, within PP_2845 at 3249.291 kb on - strand, within PP_2845 at 3249.560 kb on + strand, within PP_2845 at 3249.588 kb on - strand, within PP_2845 at 3249.599 kb on + strand, within PP_2845 at 3249.600 kb on - strand, within PP_2845 at 3249.798 kb on - strand, within PP_2845 at 3249.940 kb on - strand at 3250.001 kb on + strand at 3250.002 kb on - strand at 3250.114 kb on + strand at 3250.114 kb on + strand at 3250.114 kb on + strand at 3250.115 kb on - strand at 3250.117 kb on - strand at 3250.473 kb on + strand, within PP_2846 at 3250.473 kb on + strand, within PP_2846 at 3250.473 kb on + strand, within PP_2846 at 3250.515 kb on - strand, within PP_2846 at 3250.575 kb on + strand at 3250.990 kb on - strand, within PP_2847 at 3250.990 kb on - strand, within PP_2847
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 3,247,500 + PP_2842 0.72 +0.4 3,247,561 - PP_2842 0.79 +0.5 3,247,729 + +0.5 3,247,838 + PP_2843 0.31 +0.7 3,248,193 + PP_2844 0.40 -0.5 3,248,194 - PP_2844 0.40 +0.6 3,248,194 - PP_2844 0.40 -0.2 3,248,316 + PP_2844 0.78 -0.6 3,248,337 + PP_2844 0.85 -1.1 3,248,338 - +1.0 3,248,338 - PP_2844 0.85 +0.4 3,248,389 + +0.4 3,248,495 + +0.2 3,248,496 - +0.0 3,249,052 + PP_2845 0.39 -2.0 3,249,053 - PP_2845 0.39 -0.3 3,249,196 + PP_2845 0.48 -0.5 3,249,291 - PP_2845 0.53 -1.4 3,249,291 - PP_2845 0.53 -1.0 3,249,560 + PP_2845 0.69 -2.3 3,249,588 - PP_2845 0.71 -1.2 3,249,599 + PP_2845 0.71 +0.2 3,249,600 - PP_2845 0.72 -0.0 3,249,798 - PP_2845 0.83 -1.8 3,249,940 - -0.2 3,250,001 + -1.5 3,250,002 - -0.5 3,250,114 + +0.0 3,250,114 + +1.1 3,250,114 + -0.1 3,250,115 - +0.1 3,250,117 - -0.4 3,250,473 + PP_2846 0.75 +0.5 3,250,473 + PP_2846 0.75 +0.1 3,250,473 + PP_2846 0.75 -0.4 3,250,515 - PP_2846 0.84 -0.3 3,250,575 + -0.1 3,250,990 - PP_2847 0.68 +0.9 3,250,990 - PP_2847 0.68 +1.4
Or see this region's nucleotide sequence