Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3109 and PP_5579 are separated by 58 nucleotides PP_5579 and PP_5580 are separated by 300 nucleotides PP_5580 and PP_5582 are separated by 879 nucleotides
PP_3109: PP_3109 - conserved protein of unknown function, at 3,517,884 to 3,518,315
_3109
PP_5579: PP_5579 - conserved protein of unknown function, at 3,518,374 to 3,518,853
_5579
PP_5580: PP_5580 - protein of unknown function, at 3,519,154 to 3,519,381
_5580
PP_5582: PP_5582 - conserved protein of unknown function, at 3,520,261 to 3,520,728
_5582
Position (kb)
3519
3520 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3518.280 kb on - strand at 3518.463 kb on + strand, within PP_5579 at 3518.469 kb on + strand, within PP_5579 at 3518.616 kb on + strand, within PP_5579 at 3518.671 kb on - strand, within PP_5579 at 3518.740 kb on + strand, within PP_5579 at 3518.741 kb on - strand, within PP_5579 at 3518.758 kb on + strand, within PP_5579 at 3518.795 kb on + strand, within PP_5579 at 3518.797 kb on + strand, within PP_5579 at 3518.798 kb on - strand, within PP_5579 at 3518.851 kb on + strand at 3518.990 kb on - strand at 3519.009 kb on - strand at 3519.107 kb on + strand at 3519.119 kb on - strand at 3519.119 kb on - strand at 3519.146 kb on - strand at 3519.206 kb on + strand, within PP_5580 at 3519.323 kb on + strand, within PP_5580 at 3519.380 kb on - strand at 3519.457 kb on - strand at 3519.647 kb on - strand at 3519.690 kb on + strand at 3519.733 kb on + strand at 3519.733 kb on + strand at 3519.885 kb on + strand at 3519.924 kb on - strand at 3519.924 kb on - strand at 3519.956 kb on - strand at 3519.956 kb on - strand at 3519.958 kb on + strand at 3519.959 kb on - strand at 3519.976 kb on - strand at 3519.976 kb on - strand at 3519.999 kb on + strand at 3520.028 kb on + strand at 3520.037 kb on + strand at 3520.045 kb on + strand at 3520.083 kb on - strand at 3520.094 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 3,518,280 - -0.6 3,518,463 + PP_5579 0.19 -0.0 3,518,469 + PP_5579 0.20 +0.1 3,518,616 + PP_5579 0.50 +1.2 3,518,671 - PP_5579 0.62 +1.0 3,518,740 + PP_5579 0.76 +0.6 3,518,741 - PP_5579 0.76 +1.4 3,518,758 + PP_5579 0.80 +0.7 3,518,795 + PP_5579 0.88 +0.4 3,518,797 + PP_5579 0.88 +1.1 3,518,798 - PP_5579 0.88 -2.7 3,518,851 + -0.0 3,518,990 - -2.9 3,519,009 - +0.9 3,519,107 + -0.8 3,519,119 - +0.0 3,519,119 - -0.0 3,519,146 - +0.7 3,519,206 + PP_5580 0.23 +0.3 3,519,323 + PP_5580 0.74 +1.0 3,519,380 - -2.6 3,519,457 - -1.4 3,519,647 - +1.1 3,519,690 + +0.2 3,519,733 + -0.3 3,519,733 + +0.9 3,519,885 + -0.5 3,519,924 - +0.5 3,519,924 - +1.5 3,519,956 - +0.8 3,519,956 - -2.9 3,519,958 + -0.6 3,519,959 - -2.2 3,519,976 - +1.1 3,519,976 - +2.5 3,519,999 + -3.0 3,520,028 + +1.4 3,520,037 + -3.7 3,520,045 + -1.7 3,520,083 - +0.7 3,520,094 - -0.4
Or see this region's nucleotide sequence