Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4560 and PP_4561 are separated by 153 nucleotides PP_4561 and PP_4562 are separated by 235 nucleotides PP_4562 and PP_4563 are separated by 5 nucleotides PP_4563 and PP_4564 are separated by 130 nucleotides
PP_4560: PP_4560 - putative Ribonuclease BN, at 5,180,986 to 5,181,942
_4560
PP_4561: PP_4561 - stress response protein, at 5,182,096 to 5,182,275
_4561
PP_4562: PP_4562 - conserved protein of unknown function, at 5,182,511 to 5,182,891
_4562
PP_4563: PP_4563 - conserved protein of unknown function, at 5,182,897 to 5,183,733
_4563
PP_4564: PP_4564 - putative Lipoprotein, at 5,183,864 to 5,184,724
_4564
Position (kb) 5182
5183
5184 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 5181.942 kb on + strand at 5181.969 kb on + strand at 5181.969 kb on + strand at 5181.983 kb on + strand at 5181.983 kb on + strand at 5181.984 kb on - strand at 5181.984 kb on - strand at 5181.984 kb on - strand at 5181.986 kb on + strand at 5181.986 kb on + strand at 5181.987 kb on - strand at 5182.042 kb on + strand at 5182.042 kb on + strand at 5182.042 kb on + strand at 5182.043 kb on - strand at 5182.043 kb on - strand at 5182.187 kb on + strand, within PP_4561 at 5182.263 kb on + strand at 5182.264 kb on - strand at 5182.264 kb on - strand at 5182.381 kb on - strand at 5182.487 kb on + strand at 5182.487 kb on + strand at 5182.488 kb on - strand at 5182.881 kb on + strand at 5182.882 kb on - strand at 5182.890 kb on - strand at 5182.890 kb on - strand at 5183.301 kb on - strand, within PP_4563 at 5183.301 kb on - strand, within PP_4563 at 5183.301 kb on - strand, within PP_4563 at 5183.324 kb on + strand, within PP_4563 at 5183.375 kb on + strand, within PP_4563 at 5183.376 kb on - strand, within PP_4563 at 5183.393 kb on + strand, within PP_4563 at 5183.394 kb on - strand, within PP_4563 at 5183.394 kb on - strand, within PP_4563 at 5183.472 kb on + strand, within PP_4563 at 5183.473 kb on - strand, within PP_4563 at 5183.573 kb on + strand, within PP_4563 at 5183.573 kb on + strand, within PP_4563 at 5183.573 kb on + strand, within PP_4563 at 5183.574 kb on - strand, within PP_4563 at 5183.627 kb on + strand, within PP_4563 at 5183.627 kb on + strand, within PP_4563 at 5183.627 kb on + strand, within PP_4563 at 5183.628 kb on - strand, within PP_4563 at 5183.628 kb on - strand, within PP_4563 at 5183.628 kb on - strand at 5183.628 kb on - strand, within PP_4563 at 5183.628 kb on - strand, within PP_4563 at 5183.714 kb on + strand at 5183.766 kb on + strand at 5183.767 kb on - strand at 5183.767 kb on - strand at 5183.831 kb on + strand at 5183.831 kb on + strand at 5183.832 kb on - strand at 5183.832 kb on - strand at 5183.832 kb on - strand at 5183.871 kb on + strand at 5183.872 kb on - strand at 5183.893 kb on + strand at 5184.103 kb on - strand, within PP_4564 at 5184.246 kb on + strand, within PP_4564 at 5184.247 kb on - strand, within PP_4564 at 5184.299 kb on + strand, within PP_4564 at 5184.299 kb on + strand, within PP_4564 at 5184.299 kb on + strand, within PP_4564 at 5184.300 kb on - strand, within PP_4564 at 5184.300 kb on - strand, within PP_4564
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 5,181,942 + +3.8 5,181,969 + -1.9 5,181,969 + +0.3 5,181,983 + -0.1 5,181,983 + -2.0 5,181,984 - +0.5 5,181,984 - +1.0 5,181,984 - +0.6 5,181,986 + -1.1 5,181,986 + -0.0 5,181,987 - -2.0 5,182,042 + +0.8 5,182,042 + +0.4 5,182,042 + -0.8 5,182,043 - +0.0 5,182,043 - -0.5 5,182,187 + PP_4561 0.51 -1.0 5,182,263 + -0.0 5,182,264 - +0.7 5,182,264 - -0.7 5,182,381 - -0.2 5,182,487 + -2.0 5,182,487 + -0.5 5,182,488 - +0.6 5,182,881 + -1.3 5,182,882 - -0.5 5,182,890 - +1.3 5,182,890 - -2.2 5,183,301 - PP_4563 0.48 -3.4 5,183,301 - PP_4563 0.48 +0.2 5,183,301 - PP_4563 0.48 -1.8 5,183,324 + PP_4563 0.51 +0.3 5,183,375 + PP_4563 0.57 -0.5 5,183,376 - PP_4563 0.57 -3.2 5,183,393 + PP_4563 0.59 -1.8 5,183,394 - PP_4563 0.59 -3.1 5,183,394 - PP_4563 0.59 -4.2 5,183,472 + PP_4563 0.69 +0.6 5,183,473 - PP_4563 0.69 -1.9 5,183,573 + PP_4563 0.81 -0.4 5,183,573 + PP_4563 0.81 +0.6 5,183,573 + PP_4563 0.81 -1.1 5,183,574 - PP_4563 0.81 -0.5 5,183,627 + PP_4563 0.87 +0.9 5,183,627 + PP_4563 0.87 -0.2 5,183,627 + PP_4563 0.87 +0.1 5,183,628 - PP_4563 0.87 -0.5 5,183,628 - PP_4563 0.87 +0.4 5,183,628 - -1.4 5,183,628 - PP_4563 0.87 -0.3 5,183,628 - PP_4563 0.87 -0.3 5,183,714 + -0.3 5,183,766 + -1.5 5,183,767 - -1.2 5,183,767 - +1.1 5,183,831 + -0.4 5,183,831 + -0.7 5,183,832 - +0.3 5,183,832 - -0.0 5,183,832 - -3.7 5,183,871 + -3.7 5,183,872 - -1.1 5,183,893 + -0.1 5,184,103 - PP_4564 0.28 +0.6 5,184,246 + PP_4564 0.44 -2.3 5,184,247 - PP_4564 0.44 +1.2 5,184,299 + PP_4564 0.51 -3.1 5,184,299 + PP_4564 0.51 -1.6 5,184,299 + PP_4564 0.51 +0.6 5,184,300 - PP_4564 0.51 +0.2 5,184,300 - PP_4564 0.51 +0.2
Or see this region's nucleotide sequence