Experiment: R2A with Fusaric 240 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5595 and PP_3263 are separated by 28 nucleotides PP_3263 and PP_3264 overlap by 1 nucleotides PP_3264 and PP_3265 overlap by 4 nucleotides PP_3265 and PP_3266 overlap by 4 nucleotides
PP_5595: PP_5595 - conserved exported protein of unknown function, at 3,697,072 to 3,697,488
_5595
PP_3263: PP_3263 - phospholipid modification enzyme, at 3,697,517 to 3,698,470
_3263
PP_3264: PP_3264 - cardiolipin synthase 2, at 3,698,470 to 3,699,672
_3264
PP_3265: PP_3265 - putative phosphohydrolase of unknown function, at 3,699,669 to 3,700,442
_3265
PP_3266: PP_3266 - conserved protein of unknown function, at 3,700,439 to 3,701,491
_3266
Position (kb)
3698
3699
3700 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3697.579 kb on - strand at 3697.579 kb on - strand at 3697.611 kb on + strand at 3697.611 kb on + strand at 3697.614 kb on + strand, within PP_3263 at 3697.614 kb on + strand, within PP_3263 at 3697.615 kb on - strand, within PP_3263 at 3697.615 kb on - strand, within PP_3263 at 3697.777 kb on - strand, within PP_3263 at 3697.777 kb on - strand, within PP_3263 at 3697.941 kb on + strand, within PP_3263 at 3697.991 kb on - strand, within PP_3263 at 3698.039 kb on + strand, within PP_3263 at 3698.039 kb on + strand, within PP_3263 at 3698.039 kb on + strand at 3698.059 kb on + strand, within PP_3263 at 3698.059 kb on + strand, within PP_3263 at 3698.059 kb on + strand, within PP_3263 at 3698.060 kb on - strand, within PP_3263 at 3698.060 kb on - strand, within PP_3263 at 3698.060 kb on - strand, within PP_3263 at 3698.060 kb on - strand, within PP_3263 at 3698.061 kb on + strand, within PP_3263 at 3698.062 kb on - strand, within PP_3263 at 3698.062 kb on - strand, within PP_3263 at 3698.196 kb on + strand, within PP_3263 at 3698.219 kb on + strand, within PP_3263 at 3698.234 kb on + strand, within PP_3263 at 3698.240 kb on - strand, within PP_3263 at 3698.240 kb on - strand, within PP_3263 at 3698.271 kb on + strand, within PP_3263 at 3698.271 kb on + strand, within PP_3263 at 3698.289 kb on + strand, within PP_3263 at 3698.289 kb on + strand, within PP_3263 at 3698.290 kb on - strand, within PP_3263 at 3698.290 kb on - strand, within PP_3263 at 3698.337 kb on + strand, within PP_3263 at 3698.338 kb on - strand, within PP_3263 at 3698.392 kb on - strand at 3698.837 kb on + strand, within PP_3264 at 3698.838 kb on - strand, within PP_3264 at 3698.870 kb on + strand, within PP_3264 at 3698.871 kb on - strand at 3698.871 kb on - strand, within PP_3264 at 3698.876 kb on + strand, within PP_3264 at 3698.877 kb on - strand, within PP_3264 at 3699.053 kb on + strand, within PP_3264 at 3699.053 kb on + strand, within PP_3264 at 3699.054 kb on - strand, within PP_3264 at 3699.054 kb on - strand, within PP_3264 at 3699.234 kb on - strand, within PP_3264 at 3699.266 kb on - strand, within PP_3264 at 3699.282 kb on - strand, within PP_3264 at 3699.569 kb on - strand at 3699.569 kb on - strand at 3699.614 kb on + strand at 3699.615 kb on - strand at 3699.615 kb on - strand at 3699.615 kb on - strand at 3699.615 kb on - strand at 3699.696 kb on + strand at 3699.697 kb on - strand at 3699.725 kb on - strand at 3699.769 kb on + strand, within PP_3265 at 3699.770 kb on - strand, within PP_3265 at 3699.997 kb on - strand, within PP_3265 at 3700.163 kb on - strand, within PP_3265 at 3700.201 kb on + strand, within PP_3265 at 3700.525 kb on - strand at 3700.564 kb on - strand, within PP_3266 at 3700.564 kb on - strand, within PP_3266 at 3700.651 kb on + strand, within PP_3266
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 240 ug/mL remove 3,697,579 - +0.8 3,697,579 - -0.2 3,697,611 + -3.1 3,697,611 + -1.8 3,697,614 + PP_3263 0.10 +2.4 3,697,614 + PP_3263 0.10 -4.2 3,697,615 - PP_3263 0.10 -2.6 3,697,615 - PP_3263 0.10 +0.2 3,697,777 - PP_3263 0.27 +3.3 3,697,777 - PP_3263 0.27 +1.0 3,697,941 + PP_3263 0.44 +0.1 3,697,991 - PP_3263 0.50 +0.8 3,698,039 + PP_3263 0.55 -1.7 3,698,039 + PP_3263 0.55 -1.9 3,698,039 + -1.0 3,698,059 + PP_3263 0.57 +1.5 3,698,059 + PP_3263 0.57 -0.7 3,698,059 + PP_3263 0.57 -1.1 3,698,060 - PP_3263 0.57 +0.2 3,698,060 - PP_3263 0.57 -0.3 3,698,060 - PP_3263 0.57 -2.2 3,698,060 - PP_3263 0.57 -0.9 3,698,061 + PP_3263 0.57 -0.4 3,698,062 - PP_3263 0.57 +0.4 3,698,062 - PP_3263 0.57 -1.1 3,698,196 + PP_3263 0.71 -3.9 3,698,219 + PP_3263 0.74 +1.9 3,698,234 + PP_3263 0.75 -0.4 3,698,240 - PP_3263 0.76 -0.7 3,698,240 - PP_3263 0.76 +0.2 3,698,271 + PP_3263 0.79 -1.2 3,698,271 + PP_3263 0.79 -0.8 3,698,289 + PP_3263 0.81 -1.5 3,698,289 + PP_3263 0.81 -0.3 3,698,290 - PP_3263 0.81 -3.7 3,698,290 - PP_3263 0.81 -0.3 3,698,337 + PP_3263 0.86 +0.3 3,698,338 - PP_3263 0.86 +0.4 3,698,392 - -2.9 3,698,837 + PP_3264 0.31 +1.1 3,698,838 - PP_3264 0.31 -1.4 3,698,870 + PP_3264 0.33 +1.4 3,698,871 - +0.6 3,698,871 - PP_3264 0.33 +1.4 3,698,876 + PP_3264 0.34 +0.8 3,698,877 - PP_3264 0.34 +1.2 3,699,053 + PP_3264 0.48 -0.2 3,699,053 + PP_3264 0.48 -1.2 3,699,054 - PP_3264 0.49 -1.0 3,699,054 - PP_3264 0.49 -1.1 3,699,234 - PP_3264 0.64 +0.2 3,699,266 - PP_3264 0.66 +1.3 3,699,282 - PP_3264 0.67 +1.6 3,699,569 - -0.3 3,699,569 - +0.3 3,699,614 + -1.1 3,699,615 - +0.5 3,699,615 - -0.7 3,699,615 - -1.5 3,699,615 - +0.3 3,699,696 + -1.5 3,699,697 - -0.3 3,699,725 - +0.7 3,699,769 + PP_3265 0.13 -3.5 3,699,770 - PP_3265 0.13 -0.5 3,699,997 - PP_3265 0.42 -0.0 3,700,163 - PP_3265 0.64 -2.5 3,700,201 + PP_3265 0.69 +0.3 3,700,525 - -0.6 3,700,564 - PP_3266 0.12 -1.6 3,700,564 - PP_3266 0.12 -0.0 3,700,651 + PP_3266 0.20 -0.8
Or see this region's nucleotide sequence